Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2910 and EX28DRAFT_2911 are separated by 95 nucleotides EX28DRAFT_2911 and EX28DRAFT_2912 are separated by 118 nucleotides EX28DRAFT_2912 and EX28DRAFT_2913 are separated by 244 nucleotides
EX28DRAFT_2910: EX28DRAFT_2910 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II, at 220,927 to 223,086
_2910
EX28DRAFT_2911: EX28DRAFT_2911 - RNA, at 223,182 to 223,267
_2911
EX28DRAFT_2912: EX28DRAFT_2912 - RNA, at 223,386 to 223,466
_2912
EX28DRAFT_2913: EX28DRAFT_2913 - Transcriptional regulators, at 223,711 to 224,727
_2913
Position (kb) 223
224 Strain fitness (log2 ratio) -2
-1
0
1
2 at 222.511 kb on - strand, within EX28DRAFT_2910 at 222.511 kb on - strand, within EX28DRAFT_2910 at 222.541 kb on - strand, within EX28DRAFT_2910 at 222.597 kb on + strand, within EX28DRAFT_2910 at 222.597 kb on + strand, within EX28DRAFT_2910 at 222.597 kb on + strand, within EX28DRAFT_2910 at 222.598 kb on - strand, within EX28DRAFT_2910 at 222.656 kb on + strand, within EX28DRAFT_2910 at 222.657 kb on - strand, within EX28DRAFT_2910 at 222.735 kb on - strand, within EX28DRAFT_2910 at 222.765 kb on + strand, within EX28DRAFT_2910 at 222.766 kb on - strand, within EX28DRAFT_2910 at 222.814 kb on + strand, within EX28DRAFT_2910 at 222.891 kb on - strand at 222.912 kb on + strand at 222.912 kb on + strand at 222.912 kb on + strand at 222.913 kb on - strand at 222.913 kb on - strand at 222.913 kb on - strand at 222.913 kb on - strand at 222.914 kb on + strand at 222.914 kb on + strand at 222.914 kb on + strand at 222.914 kb on + strand at 222.915 kb on - strand at 222.946 kb on + strand at 222.968 kb on - strand at 223.054 kb on - strand at 223.065 kb on + strand at 223.082 kb on - strand at 223.082 kb on - strand at 223.123 kb on - strand at 223.126 kb on - strand at 223.126 kb on - strand at 223.126 kb on - strand at 223.141 kb on - strand at 223.226 kb on - strand, within EX28DRAFT_2911 at 223.235 kb on + strand, within EX28DRAFT_2911 at 223.236 kb on - strand, within EX28DRAFT_2911 at 223.349 kb on - strand at 223.419 kb on - strand, within EX28DRAFT_2912 at 223.419 kb on - strand, within EX28DRAFT_2912 at 223.451 kb on + strand, within EX28DRAFT_2912 at 223.452 kb on - strand, within EX28DRAFT_2912 at 223.457 kb on + strand, within EX28DRAFT_2912 at 223.458 kb on - strand, within EX28DRAFT_2912 at 223.458 kb on - strand, within EX28DRAFT_2912 at 223.458 kb on - strand, within EX28DRAFT_2912 at 223.478 kb on - strand at 223.490 kb on - strand at 223.492 kb on + strand at 223.493 kb on - strand at 223.493 kb on - strand at 223.493 kb on - strand at 223.534 kb on + strand at 223.844 kb on - strand, within EX28DRAFT_2913 at 223.844 kb on - strand, within EX28DRAFT_2913 at 223.844 kb on - strand, within EX28DRAFT_2913 at 224.007 kb on + strand, within EX28DRAFT_2913 at 224.008 kb on - strand, within EX28DRAFT_2913 at 224.306 kb on - strand, within EX28DRAFT_2913 at 224.440 kb on + strand, within EX28DRAFT_2913
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 222,511 - EX28DRAFT_2910 0.73 +0.8 222,511 - EX28DRAFT_2910 0.73 +1.8 222,541 - EX28DRAFT_2910 0.75 +0.0 222,597 + EX28DRAFT_2910 0.77 +0.8 222,597 + EX28DRAFT_2910 0.77 -0.1 222,597 + EX28DRAFT_2910 0.77 -0.4 222,598 - EX28DRAFT_2910 0.77 +0.7 222,656 + EX28DRAFT_2910 0.80 +0.2 222,657 - EX28DRAFT_2910 0.80 -0.2 222,735 - EX28DRAFT_2910 0.84 -0.5 222,765 + EX28DRAFT_2910 0.85 +0.1 222,766 - EX28DRAFT_2910 0.85 +0.1 222,814 + EX28DRAFT_2910 0.87 -0.1 222,891 - +1.3 222,912 + +0.0 222,912 + -2.5 222,912 + -0.2 222,913 - +0.3 222,913 - -0.3 222,913 - +0.3 222,913 - -0.0 222,914 + -0.5 222,914 + -0.2 222,914 + -0.7 222,914 + -0.1 222,915 - +0.8 222,946 + +0.6 222,968 - -0.1 223,054 - +0.3 223,065 + -0.6 223,082 - -0.5 223,082 - -0.6 223,123 - +0.3 223,126 - +0.8 223,126 - -0.5 223,126 - +0.2 223,141 - -0.9 223,226 - EX28DRAFT_2911 0.51 -0.2 223,235 + EX28DRAFT_2911 0.62 +0.2 223,236 - EX28DRAFT_2911 0.63 -1.5 223,349 - -0.3 223,419 - EX28DRAFT_2912 0.41 -0.5 223,419 - EX28DRAFT_2912 0.41 +0.2 223,451 + EX28DRAFT_2912 0.80 -1.1 223,452 - EX28DRAFT_2912 0.81 -0.1 223,457 + EX28DRAFT_2912 0.88 -0.1 223,458 - EX28DRAFT_2912 0.89 +0.1 223,458 - EX28DRAFT_2912 0.89 +0.2 223,458 - EX28DRAFT_2912 0.89 +0.2 223,478 - +0.8 223,490 - +0.4 223,492 + -1.0 223,493 - +0.0 223,493 - -1.2 223,493 - -0.1 223,534 + +0.7 223,844 - EX28DRAFT_2913 0.13 -0.4 223,844 - EX28DRAFT_2913 0.13 -1.8 223,844 - EX28DRAFT_2913 0.13 +0.6 224,007 + EX28DRAFT_2913 0.29 +0.4 224,008 - EX28DRAFT_2913 0.29 +0.8 224,306 - EX28DRAFT_2913 0.59 +0.3 224,440 + EX28DRAFT_2913 0.72 -0.2
Or see this region's nucleotide sequence