Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2889 and EX28DRAFT_2890 are separated by 208 nucleotides EX28DRAFT_2890 and EX28DRAFT_2891 are separated by 44 nucleotides EX28DRAFT_2891 and EX28DRAFT_2892 are separated by 88 nucleotides
EX28DRAFT_2889: EX28DRAFT_2889 - Membrane-bound lytic murein transglycosylase, at 193,997 to 195,094
_2889
EX28DRAFT_2890: EX28DRAFT_2890 - tRNA, at 195,303 to 195,379
_2890
EX28DRAFT_2891: EX28DRAFT_2891 - tRNA, at 195,424 to 195,500
_2891
EX28DRAFT_2892: EX28DRAFT_2892 - N-acetylmuramoyl-L-alanine amidase, at 195,589 to 196,857
_2892
Position (kb) 195
196 Strain fitness (log2 ratio) -1
0
1
2 at 194.496 kb on + strand, within EX28DRAFT_2889 at 194.496 kb on + strand, within EX28DRAFT_2889 at 194.497 kb on - strand, within EX28DRAFT_2889 at 194.538 kb on + strand, within EX28DRAFT_2889 at 194.589 kb on + strand, within EX28DRAFT_2889 at 194.602 kb on - strand, within EX28DRAFT_2889 at 194.668 kb on + strand, within EX28DRAFT_2889 at 194.673 kb on + strand, within EX28DRAFT_2889 at 194.674 kb on - strand, within EX28DRAFT_2889 at 194.677 kb on + strand, within EX28DRAFT_2889 at 194.678 kb on - strand, within EX28DRAFT_2889 at 194.730 kb on + strand, within EX28DRAFT_2889 at 194.730 kb on + strand, within EX28DRAFT_2889 at 194.806 kb on + strand, within EX28DRAFT_2889 at 194.806 kb on + strand, within EX28DRAFT_2889 at 194.853 kb on + strand, within EX28DRAFT_2889 at 194.853 kb on + strand, within EX28DRAFT_2889 at 194.854 kb on - strand, within EX28DRAFT_2889 at 194.854 kb on - strand, within EX28DRAFT_2889 at 194.860 kb on + strand, within EX28DRAFT_2889 at 194.874 kb on + strand, within EX28DRAFT_2889 at 194.967 kb on - strand, within EX28DRAFT_2889 at 195.000 kb on + strand at 195.001 kb on - strand at 195.001 kb on - strand at 195.001 kb on - strand at 195.073 kb on - strand at 195.095 kb on + strand at 195.095 kb on + strand at 195.096 kb on - strand at 195.096 kb on - strand at 195.096 kb on - strand at 195.098 kb on - strand at 195.098 kb on - strand at 195.098 kb on - strand at 195.098 kb on - strand at 195.098 kb on - strand at 195.098 kb on - strand at 195.248 kb on - strand at 195.339 kb on + strand, within EX28DRAFT_2890 at 195.397 kb on - strand at 195.502 kb on + strand at 195.503 kb on - strand at 195.517 kb on + strand at 195.690 kb on + strand at 195.691 kb on - strand at 195.792 kb on + strand, within EX28DRAFT_2892 at 195.885 kb on + strand, within EX28DRAFT_2892 at 195.885 kb on + strand, within EX28DRAFT_2892 at 195.885 kb on + strand, within EX28DRAFT_2892 at 195.885 kb on + strand, within EX28DRAFT_2892 at 195.886 kb on - strand, within EX28DRAFT_2892 at 195.886 kb on - strand, within EX28DRAFT_2892 at 195.886 kb on - strand, within EX28DRAFT_2892 at 196.119 kb on + strand, within EX28DRAFT_2892 at 196.137 kb on + strand, within EX28DRAFT_2892 at 196.137 kb on + strand, within EX28DRAFT_2892 at 196.140 kb on - strand, within EX28DRAFT_2892 at 196.140 kb on - strand, within EX28DRAFT_2892 at 196.149 kb on + strand, within EX28DRAFT_2892 at 196.361 kb on + strand, within EX28DRAFT_2892
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 194,496 + EX28DRAFT_2889 0.45 +1.0 194,496 + EX28DRAFT_2889 0.45 -0.6 194,497 - EX28DRAFT_2889 0.46 -0.7 194,538 + EX28DRAFT_2889 0.49 +0.4 194,589 + EX28DRAFT_2889 0.54 -0.1 194,602 - EX28DRAFT_2889 0.55 -0.2 194,668 + EX28DRAFT_2889 0.61 -0.1 194,673 + EX28DRAFT_2889 0.62 +0.8 194,674 - EX28DRAFT_2889 0.62 +0.1 194,677 + EX28DRAFT_2889 0.62 +1.4 194,678 - EX28DRAFT_2889 0.62 -0.5 194,730 + EX28DRAFT_2889 0.67 +0.3 194,730 + EX28DRAFT_2889 0.67 +0.6 194,806 + EX28DRAFT_2889 0.74 -0.5 194,806 + EX28DRAFT_2889 0.74 +1.3 194,853 + EX28DRAFT_2889 0.78 -0.3 194,853 + EX28DRAFT_2889 0.78 +0.5 194,854 - EX28DRAFT_2889 0.78 +0.3 194,854 - EX28DRAFT_2889 0.78 +0.8 194,860 + EX28DRAFT_2889 0.79 -0.1 194,874 + EX28DRAFT_2889 0.80 +0.0 194,967 - EX28DRAFT_2889 0.88 +2.2 195,000 + +0.5 195,001 - +0.0 195,001 - +0.4 195,001 - +0.2 195,073 - +1.5 195,095 + -0.1 195,095 + +0.5 195,096 - -0.2 195,096 - +0.4 195,096 - -0.2 195,098 - -0.1 195,098 - +0.7 195,098 - +0.2 195,098 - -0.8 195,098 - +0.4 195,098 - +0.1 195,248 - -0.4 195,339 + EX28DRAFT_2890 0.47 +0.4 195,397 - +0.5 195,502 + -0.0 195,503 - -0.4 195,517 + +0.2 195,690 + +0.6 195,691 - +1.4 195,792 + EX28DRAFT_2892 0.16 +0.3 195,885 + EX28DRAFT_2892 0.23 +0.1 195,885 + EX28DRAFT_2892 0.23 -0.0 195,885 + EX28DRAFT_2892 0.23 +0.0 195,885 + EX28DRAFT_2892 0.23 -0.9 195,886 - EX28DRAFT_2892 0.23 +0.4 195,886 - EX28DRAFT_2892 0.23 +0.9 195,886 - EX28DRAFT_2892 0.23 -0.4 196,119 + EX28DRAFT_2892 0.42 +0.0 196,137 + EX28DRAFT_2892 0.43 +0.8 196,137 + EX28DRAFT_2892 0.43 +0.1 196,140 - EX28DRAFT_2892 0.43 -0.2 196,140 - EX28DRAFT_2892 0.43 +0.3 196,149 + EX28DRAFT_2892 0.44 -0.4 196,361 + EX28DRAFT_2892 0.61 +0.8
Or see this region's nucleotide sequence