Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1876 and EX28DRAFT_1877 are separated by 221 nucleotides EX28DRAFT_1877 and EX28DRAFT_1878 are separated by 140 nucleotides
EX28DRAFT_1876: EX28DRAFT_1876 - Predicted sulfurtransferase, at 1,887,713 to 1,888,762
_1876
EX28DRAFT_1877: EX28DRAFT_1877 - lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase, at 1,888,984 to 1,889,910
_1877
EX28DRAFT_1878: EX28DRAFT_1878 - Arabinose efflux permease, at 1,890,051 to 1,891,268
_1878
Position (kb)
1888
1889
1890 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1887.989 kb on - strand, within EX28DRAFT_1876 at 1888.108 kb on + strand, within EX28DRAFT_1876 at 1888.108 kb on + strand, within EX28DRAFT_1876 at 1888.109 kb on - strand, within EX28DRAFT_1876 at 1888.167 kb on + strand, within EX28DRAFT_1876 at 1888.168 kb on - strand, within EX28DRAFT_1876 at 1888.168 kb on - strand, within EX28DRAFT_1876 at 1888.168 kb on - strand, within EX28DRAFT_1876 at 1888.171 kb on + strand, within EX28DRAFT_1876 at 1888.172 kb on - strand, within EX28DRAFT_1876 at 1888.172 kb on - strand, within EX28DRAFT_1876 at 1888.183 kb on + strand, within EX28DRAFT_1876 at 1888.190 kb on - strand, within EX28DRAFT_1876 at 1888.282 kb on - strand, within EX28DRAFT_1876 at 1888.282 kb on - strand, within EX28DRAFT_1876 at 1888.301 kb on - strand, within EX28DRAFT_1876 at 1888.359 kb on + strand, within EX28DRAFT_1876 at 1888.359 kb on + strand, within EX28DRAFT_1876 at 1888.475 kb on - strand, within EX28DRAFT_1876 at 1888.555 kb on + strand, within EX28DRAFT_1876 at 1888.587 kb on + strand, within EX28DRAFT_1876 at 1888.668 kb on + strand at 1888.668 kb on + strand at 1888.668 kb on + strand at 1888.684 kb on + strand at 1888.685 kb on - strand at 1888.751 kb on + strand at 1888.767 kb on + strand at 1888.767 kb on + strand at 1888.768 kb on - strand at 1888.795 kb on - strand at 1888.816 kb on - strand at 1888.816 kb on - strand at 1888.834 kb on - strand at 1888.930 kb on - strand at 1889.092 kb on - strand, within EX28DRAFT_1877 at 1889.216 kb on - strand, within EX28DRAFT_1877 at 1889.578 kb on + strand, within EX28DRAFT_1877 at 1889.616 kb on - strand, within EX28DRAFT_1877 at 1889.851 kb on + strand at 1889.853 kb on + strand at 1889.853 kb on + strand at 1889.854 kb on - strand at 1889.854 kb on - strand at 1889.854 kb on - strand at 1889.854 kb on - strand at 1889.908 kb on + strand at 1889.909 kb on - strand at 1889.951 kb on - strand at 1889.978 kb on + strand at 1889.996 kb on - strand at 1890.050 kb on + strand at 1890.050 kb on + strand at 1890.050 kb on + strand at 1890.050 kb on + strand at 1890.050 kb on + strand at 1890.050 kb on + strand at 1890.051 kb on - strand at 1890.051 kb on - strand at 1890.051 kb on - strand at 1890.051 kb on - strand at 1890.051 kb on - strand at 1890.051 kb on - strand at 1890.051 kb on - strand at 1890.051 kb on - strand at 1890.051 kb on - strand at 1890.203 kb on + strand, within EX28DRAFT_1878 at 1890.204 kb on - strand, within EX28DRAFT_1878 at 1890.220 kb on + strand, within EX28DRAFT_1878 at 1890.293 kb on + strand, within EX28DRAFT_1878 at 1890.294 kb on - strand, within EX28DRAFT_1878 at 1890.333 kb on - strand, within EX28DRAFT_1878 at 1890.413 kb on + strand, within EX28DRAFT_1878 at 1890.414 kb on - strand, within EX28DRAFT_1878 at 1890.742 kb on + strand, within EX28DRAFT_1878 at 1890.743 kb on - strand, within EX28DRAFT_1878 at 1890.765 kb on - strand, within EX28DRAFT_1878 at 1890.777 kb on + strand, within EX28DRAFT_1878 at 1890.778 kb on - strand, within EX28DRAFT_1878 at 1890.797 kb on + strand, within EX28DRAFT_1878 at 1890.810 kb on - strand, within EX28DRAFT_1878 at 1890.810 kb on - strand, within EX28DRAFT_1878
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,887,989 - EX28DRAFT_1876 0.26 +0.8 1,888,108 + EX28DRAFT_1876 0.38 -0.5 1,888,108 + EX28DRAFT_1876 0.38 +0.5 1,888,109 - EX28DRAFT_1876 0.38 -0.3 1,888,167 + EX28DRAFT_1876 0.43 -0.6 1,888,168 - EX28DRAFT_1876 0.43 -1.0 1,888,168 - EX28DRAFT_1876 0.43 +1.4 1,888,168 - EX28DRAFT_1876 0.43 -0.1 1,888,171 + EX28DRAFT_1876 0.44 +0.6 1,888,172 - EX28DRAFT_1876 0.44 +0.1 1,888,172 - EX28DRAFT_1876 0.44 +0.7 1,888,183 + EX28DRAFT_1876 0.45 +0.2 1,888,190 - EX28DRAFT_1876 0.45 +0.8 1,888,282 - EX28DRAFT_1876 0.54 +1.4 1,888,282 - EX28DRAFT_1876 0.54 +0.7 1,888,301 - EX28DRAFT_1876 0.56 -1.1 1,888,359 + EX28DRAFT_1876 0.62 -1.1 1,888,359 + EX28DRAFT_1876 0.62 +0.5 1,888,475 - EX28DRAFT_1876 0.73 +1.5 1,888,555 + EX28DRAFT_1876 0.80 +0.4 1,888,587 + EX28DRAFT_1876 0.83 +0.4 1,888,668 + +0.3 1,888,668 + -1.3 1,888,668 + -1.4 1,888,684 + -0.3 1,888,685 - +0.6 1,888,751 + -0.0 1,888,767 + -0.4 1,888,767 + -0.5 1,888,768 - +2.0 1,888,795 - +0.1 1,888,816 - +0.4 1,888,816 - -2.3 1,888,834 - +0.2 1,888,930 - +1.7 1,889,092 - EX28DRAFT_1877 0.12 +0.7 1,889,216 - EX28DRAFT_1877 0.25 +0.4 1,889,578 + EX28DRAFT_1877 0.64 +0.7 1,889,616 - EX28DRAFT_1877 0.68 +2.2 1,889,851 + -0.3 1,889,853 + -0.2 1,889,853 + +0.7 1,889,854 - -0.9 1,889,854 - +0.8 1,889,854 - +0.5 1,889,854 - +0.1 1,889,908 + +0.0 1,889,909 - -0.3 1,889,951 - +0.1 1,889,978 + -0.2 1,889,996 - -0.1 1,890,050 + +0.1 1,890,050 + +0.7 1,890,050 + -0.5 1,890,050 + +0.3 1,890,050 + -1.3 1,890,050 + +0.3 1,890,051 - -1.9 1,890,051 - -0.5 1,890,051 - -1.2 1,890,051 - +0.5 1,890,051 - -0.0 1,890,051 - -1.0 1,890,051 - -0.1 1,890,051 - -0.2 1,890,051 - -0.1 1,890,203 + EX28DRAFT_1878 0.12 +0.5 1,890,204 - EX28DRAFT_1878 0.13 +0.3 1,890,220 + EX28DRAFT_1878 0.14 +0.4 1,890,293 + EX28DRAFT_1878 0.20 +0.1 1,890,294 - EX28DRAFT_1878 0.20 +0.3 1,890,333 - EX28DRAFT_1878 0.23 +0.5 1,890,413 + EX28DRAFT_1878 0.30 +0.0 1,890,414 - EX28DRAFT_1878 0.30 +0.8 1,890,742 + EX28DRAFT_1878 0.57 -0.2 1,890,743 - EX28DRAFT_1878 0.57 -0.8 1,890,765 - EX28DRAFT_1878 0.59 -0.3 1,890,777 + EX28DRAFT_1878 0.60 +0.5 1,890,778 - EX28DRAFT_1878 0.60 +0.2 1,890,797 + EX28DRAFT_1878 0.61 -0.3 1,890,810 - EX28DRAFT_1878 0.62 -0.3 1,890,810 - EX28DRAFT_1878 0.62 +0.9
Or see this region's nucleotide sequence