Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1642 and EX28DRAFT_1643 overlap by 22 nucleotides EX28DRAFT_1643 and EX28DRAFT_1644 are separated by 80 nucleotides EX28DRAFT_1644 and EX28DRAFT_1645 are separated by 48 nucleotides EX28DRAFT_1645 and EX28DRAFT_1646 are separated by 149 nucleotides
EX28DRAFT_1642: EX28DRAFT_1642 - Predicted membrane protein, at 1,658,544 to 1,660,577
_1642
EX28DRAFT_1643: EX28DRAFT_1643 - Cu/Zn superoxide dismutase, at 1,660,556 to 1,661,074
_1643
EX28DRAFT_1644: EX28DRAFT_1644 - Predicted oxidoreductase, at 1,661,155 to 1,662,051
_1644
EX28DRAFT_1645: EX28DRAFT_1645 - Predicted Fe-S protein, at 1,662,100 to 1,662,339
_1645
EX28DRAFT_1646: EX28DRAFT_1646 - Predicted membrane-associated, metal-dependent hydrolase, at 1,662,489 to 1,664,114
_1646
Position (kb)
1661
1662
1663 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1660.194 kb on + strand, within EX28DRAFT_1642 at 1660.195 kb on - strand, within EX28DRAFT_1642 at 1660.372 kb on + strand, within EX28DRAFT_1642 at 1660.480 kb on + strand at 1660.481 kb on - strand at 1660.487 kb on + strand at 1660.487 kb on + strand at 1660.488 kb on - strand at 1660.490 kb on - strand at 1660.490 kb on - strand at 1660.490 kb on - strand at 1660.618 kb on + strand, within EX28DRAFT_1643 at 1660.619 kb on - strand, within EX28DRAFT_1643 at 1661.074 kb on + strand at 1661.075 kb on - strand at 1661.104 kb on + strand at 1661.110 kb on + strand at 1661.312 kb on + strand, within EX28DRAFT_1644 at 1661.312 kb on + strand, within EX28DRAFT_1644 at 1661.756 kb on + strand, within EX28DRAFT_1644 at 1661.889 kb on - strand, within EX28DRAFT_1644 at 1661.893 kb on - strand, within EX28DRAFT_1644 at 1661.967 kb on + strand at 1661.967 kb on + strand at 1661.968 kb on - strand at 1661.968 kb on - strand at 1661.968 kb on - strand at 1661.990 kb on + strand at 1661.991 kb on - strand at 1661.999 kb on - strand at 1662.036 kb on + strand at 1662.071 kb on + strand at 1662.071 kb on + strand at 1662.093 kb on + strand at 1662.403 kb on + strand at 1662.403 kb on + strand at 1662.403 kb on + strand at 1662.403 kb on + strand at 1662.403 kb on + strand at 1662.404 kb on - strand at 1662.404 kb on - strand at 1662.449 kb on + strand at 1662.652 kb on + strand, within EX28DRAFT_1646 at 1662.653 kb on - strand, within EX28DRAFT_1646 at 1662.686 kb on + strand, within EX28DRAFT_1646 at 1662.697 kb on - strand, within EX28DRAFT_1646 at 1662.722 kb on - strand, within EX28DRAFT_1646 at 1662.750 kb on + strand, within EX28DRAFT_1646 at 1662.750 kb on + strand, within EX28DRAFT_1646 at 1662.750 kb on + strand, within EX28DRAFT_1646 at 1662.751 kb on - strand, within EX28DRAFT_1646 at 1662.755 kb on + strand, within EX28DRAFT_1646 at 1662.755 kb on + strand, within EX28DRAFT_1646 at 1662.756 kb on - strand, within EX28DRAFT_1646 at 1662.756 kb on - strand, within EX28DRAFT_1646 at 1662.756 kb on - strand, within EX28DRAFT_1646 at 1662.756 kb on - strand, within EX28DRAFT_1646 at 1662.759 kb on + strand, within EX28DRAFT_1646 at 1662.759 kb on + strand, within EX28DRAFT_1646 at 1662.759 kb on + strand, within EX28DRAFT_1646 at 1662.760 kb on - strand, within EX28DRAFT_1646 at 1662.760 kb on - strand, within EX28DRAFT_1646 at 1662.760 kb on - strand, within EX28DRAFT_1646 at 1662.760 kb on - strand, within EX28DRAFT_1646 at 1662.766 kb on - strand, within EX28DRAFT_1646 at 1662.766 kb on - strand, within EX28DRAFT_1646 at 1663.005 kb on - strand, within EX28DRAFT_1646 at 1663.012 kb on - strand, within EX28DRAFT_1646
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,660,194 + EX28DRAFT_1642 0.81 +0.0 1,660,195 - EX28DRAFT_1642 0.81 +0.2 1,660,372 + EX28DRAFT_1642 0.90 -0.5 1,660,480 + +0.1 1,660,481 - -1.2 1,660,487 + -0.0 1,660,487 + -0.1 1,660,488 - +0.6 1,660,490 - -0.4 1,660,490 - +0.2 1,660,490 - +0.7 1,660,618 + EX28DRAFT_1643 0.12 -1.3 1,660,619 - EX28DRAFT_1643 0.12 -0.1 1,661,074 + +0.7 1,661,075 - +1.4 1,661,104 + +0.2 1,661,110 + -0.3 1,661,312 + EX28DRAFT_1644 0.18 +1.5 1,661,312 + EX28DRAFT_1644 0.18 +0.4 1,661,756 + EX28DRAFT_1644 0.67 -0.1 1,661,889 - EX28DRAFT_1644 0.82 -0.3 1,661,893 - EX28DRAFT_1644 0.82 -3.4 1,661,967 + +0.1 1,661,967 + +0.3 1,661,968 - +1.3 1,661,968 - +1.3 1,661,968 - -0.0 1,661,990 + -0.1 1,661,991 - -0.1 1,661,999 - +0.7 1,662,036 + -0.2 1,662,071 + +2.1 1,662,071 + +0.2 1,662,093 + -1.8 1,662,403 + -0.5 1,662,403 + +0.7 1,662,403 + -0.3 1,662,403 + +0.8 1,662,403 + +0.6 1,662,404 - +1.1 1,662,404 - +0.0 1,662,449 + -0.0 1,662,652 + EX28DRAFT_1646 0.10 +0.6 1,662,653 - EX28DRAFT_1646 0.10 -0.1 1,662,686 + EX28DRAFT_1646 0.12 -1.5 1,662,697 - EX28DRAFT_1646 0.13 +1.0 1,662,722 - EX28DRAFT_1646 0.14 -0.3 1,662,750 + EX28DRAFT_1646 0.16 +0.1 1,662,750 + EX28DRAFT_1646 0.16 -0.8 1,662,750 + EX28DRAFT_1646 0.16 -0.2 1,662,751 - EX28DRAFT_1646 0.16 -0.6 1,662,755 + EX28DRAFT_1646 0.16 +0.4 1,662,755 + EX28DRAFT_1646 0.16 -0.5 1,662,756 - EX28DRAFT_1646 0.16 +0.5 1,662,756 - EX28DRAFT_1646 0.16 -1.2 1,662,756 - EX28DRAFT_1646 0.16 -0.3 1,662,756 - EX28DRAFT_1646 0.16 +0.2 1,662,759 + EX28DRAFT_1646 0.17 +0.3 1,662,759 + EX28DRAFT_1646 0.17 +0.5 1,662,759 + EX28DRAFT_1646 0.17 +0.8 1,662,760 - EX28DRAFT_1646 0.17 -3.5 1,662,760 - EX28DRAFT_1646 0.17 +0.1 1,662,760 - EX28DRAFT_1646 0.17 +0.1 1,662,760 - EX28DRAFT_1646 0.17 +0.0 1,662,766 - EX28DRAFT_1646 0.17 +0.5 1,662,766 - EX28DRAFT_1646 0.17 +1.0 1,663,005 - EX28DRAFT_1646 0.32 +0.1 1,663,012 - EX28DRAFT_1646 0.32 -0.2
Or see this region's nucleotide sequence