Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0066 and EX28DRAFT_0067 are separated by 40 nucleotides EX28DRAFT_0067 and EX28DRAFT_0068 are separated by 313 nucleotides EX28DRAFT_0068 and EX28DRAFT_0069 are separated by 50 nucleotides
EX28DRAFT_0066: EX28DRAFT_0066 - rRNA methylases, at 7,739 to 8,869
_0066
EX28DRAFT_0067: EX28DRAFT_0067 - Uracil-DNA glycosylase (EC 3.2.2.-), at 8,910 to 9,599
_0067
EX28DRAFT_0068: EX28DRAFT_0068 - autonomous glycyl radical cofactor GrcA, at 9,913 to 10,296
_0068
EX28DRAFT_0069: EX28DRAFT_0069 - Superfamily II DNA and RNA helicases, at 10,347 to 11,675
_0069
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 8.210 kb on - strand, within EX28DRAFT_0066 at 8.321 kb on - strand, within EX28DRAFT_0066 at 8.555 kb on - strand, within EX28DRAFT_0066 at 8.580 kb on + strand, within EX28DRAFT_0066 at 8.629 kb on + strand, within EX28DRAFT_0066 at 8.715 kb on + strand, within EX28DRAFT_0066 at 8.716 kb on - strand, within EX28DRAFT_0066 at 8.867 kb on + strand at 8.867 kb on + strand at 8.868 kb on - strand at 8.876 kb on + strand at 8.912 kb on - strand at 8.928 kb on + strand at 8.931 kb on + strand at 8.931 kb on + strand at 9.120 kb on + strand, within EX28DRAFT_0067 at 9.146 kb on + strand, within EX28DRAFT_0067 at 9.230 kb on + strand, within EX28DRAFT_0067 at 9.321 kb on - strand, within EX28DRAFT_0067 at 9.322 kb on + strand, within EX28DRAFT_0067 at 9.322 kb on + strand, within EX28DRAFT_0067 at 9.322 kb on + strand, within EX28DRAFT_0067 at 9.322 kb on + strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.539 kb on + strand at 9.540 kb on - strand at 9.544 kb on + strand at 9.545 kb on - strand at 9.631 kb on - strand at 9.635 kb on + strand at 9.830 kb on + strand at 9.830 kb on + strand at 9.831 kb on - strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.833 kb on - strand at 9.833 kb on - strand at 9.833 kb on - strand at 9.834 kb on + strand at 9.834 kb on + strand at 9.834 kb on + strand at 9.835 kb on - strand at 9.836 kb on + strand at 9.889 kb on + strand at 9.889 kb on + strand at 9.890 kb on - strand at 9.890 kb on - strand at 9.895 kb on - strand at 9.918 kb on + strand at 9.919 kb on - strand at 10.072 kb on + strand, within EX28DRAFT_0068 at 10.137 kb on - strand, within EX28DRAFT_0068 at 10.275 kb on + strand at 10.403 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 8,210 - EX28DRAFT_0066 0.42 +0.5 8,321 - EX28DRAFT_0066 0.51 +1.7 8,555 - EX28DRAFT_0066 0.72 -0.1 8,580 + EX28DRAFT_0066 0.74 +0.4 8,629 + EX28DRAFT_0066 0.79 -0.2 8,715 + EX28DRAFT_0066 0.86 +0.0 8,716 - EX28DRAFT_0066 0.86 -1.3 8,867 + -0.3 8,867 + +0.2 8,868 - -0.6 8,876 + +0.3 8,912 - -1.7 8,928 + +0.5 8,931 + -0.4 8,931 + +0.2 9,120 + EX28DRAFT_0067 0.30 -1.7 9,146 + EX28DRAFT_0067 0.34 +0.6 9,230 + EX28DRAFT_0067 0.46 +0.6 9,321 - EX28DRAFT_0067 0.60 +0.1 9,322 + EX28DRAFT_0067 0.60 -1.1 9,322 + EX28DRAFT_0067 0.60 +1.1 9,322 + EX28DRAFT_0067 0.60 -0.1 9,322 + EX28DRAFT_0067 0.60 +0.4 9,323 - EX28DRAFT_0067 0.60 +0.4 9,323 - EX28DRAFT_0067 0.60 +0.3 9,323 - EX28DRAFT_0067 0.60 -0.1 9,323 - EX28DRAFT_0067 0.60 +0.0 9,323 - EX28DRAFT_0067 0.60 -1.0 9,539 + +0.5 9,540 - -3.4 9,544 + -0.3 9,545 - -0.9 9,631 - +1.5 9,635 + -0.8 9,830 + -0.6 9,830 + +0.3 9,831 - -1.0 9,832 + +0.6 9,832 + +0.1 9,832 + +0.3 9,832 + +0.0 9,832 + +0.1 9,832 + +3.4 9,832 + -0.6 9,832 + -0.3 9,833 - +0.4 9,833 - +0.2 9,833 - -0.5 9,834 + -0.2 9,834 + -0.4 9,834 + +0.0 9,835 - +0.8 9,836 + -3.2 9,889 + -0.8 9,889 + -0.7 9,890 - -1.8 9,890 - +0.3 9,895 - -0.3 9,918 + +1.0 9,919 - -1.6 10,072 + EX28DRAFT_0068 0.41 -1.9 10,137 - EX28DRAFT_0068 0.58 -0.7 10,275 + +1.7 10,403 + -1.1
Or see this region's nucleotide sequence