Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4408 and EX28DRAFT_4409 are separated by 200 nucleotides EX28DRAFT_4409 and EX28DRAFT_4410 are separated by 48 nucleotides EX28DRAFT_4410 and EX28DRAFT_4411 are separated by 101 nucleotides EX28DRAFT_4411 and EX28DRAFT_4412 are separated by 2 nucleotides
EX28DRAFT_4408: EX28DRAFT_4408 - replication restart DNA helicase PriA, at 70,409 to 72,604
_4408
EX28DRAFT_4409: EX28DRAFT_4409 - ribosomal protein L31, at 72,805 to 73,017
_4409
EX28DRAFT_4410: EX28DRAFT_4410 - methionine repressor, MetJ, at 73,066 to 73,383
_4410
EX28DRAFT_4411: EX28DRAFT_4411 - cystathionine gamma-synthase (EC 2.5.1.48), at 73,485 to 74,820
_4411
EX28DRAFT_4412: EX28DRAFT_4412 - aspartate kinase (EC 2.7.2.4), at 74,823 to 77,255
_4412
Position (kb) 73
74
75 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 72.635 kb on + strand at 72.636 kb on - strand at 72.638 kb on + strand at 72.638 kb on + strand at 72.639 kb on - strand at 72.640 kb on + strand at 72.641 kb on - strand at 72.641 kb on - strand at 72.641 kb on - strand at 72.647 kb on + strand at 72.648 kb on - strand at 72.650 kb on + strand at 73.141 kb on - strand, within EX28DRAFT_4410 at 73.360 kb on - strand at 73.558 kb on + strand at 73.588 kb on + strand at 73.613 kb on + strand at 73.668 kb on + strand, within EX28DRAFT_4411 at 73.681 kb on - strand, within EX28DRAFT_4411 at 73.756 kb on + strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.815 kb on + strand, within EX28DRAFT_4411 at 73.871 kb on + strand, within EX28DRAFT_4411 at 73.872 kb on - strand, within EX28DRAFT_4411 at 73.872 kb on - strand, within EX28DRAFT_4411 at 73.960 kb on + strand, within EX28DRAFT_4411 at 73.972 kb on + strand, within EX28DRAFT_4411 at 74.166 kb on - strand, within EX28DRAFT_4411 at 74.166 kb on - strand, within EX28DRAFT_4411 at 74.172 kb on - strand, within EX28DRAFT_4411 at 74.181 kb on + strand, within EX28DRAFT_4411 at 74.181 kb on + strand, within EX28DRAFT_4411 at 74.181 kb on + strand, within EX28DRAFT_4411 at 74.182 kb on - strand, within EX28DRAFT_4411 at 74.182 kb on - strand, within EX28DRAFT_4411 at 74.249 kb on + strand, within EX28DRAFT_4411 at 74.250 kb on + strand, within EX28DRAFT_4411 at 74.250 kb on + strand, within EX28DRAFT_4411 at 74.250 kb on + strand, within EX28DRAFT_4411 at 74.251 kb on - strand, within EX28DRAFT_4411 at 74.254 kb on + strand, within EX28DRAFT_4411 at 74.341 kb on - strand, within EX28DRAFT_4411 at 74.374 kb on + strand, within EX28DRAFT_4411 at 74.374 kb on + strand, within EX28DRAFT_4411 at 74.375 kb on - strand, within EX28DRAFT_4411 at 74.375 kb on - strand, within EX28DRAFT_4411 at 74.486 kb on - strand, within EX28DRAFT_4411 at 74.558 kb on + strand, within EX28DRAFT_4411 at 74.558 kb on + strand, within EX28DRAFT_4411 at 74.623 kb on - strand, within EX28DRAFT_4411 at 74.640 kb on - strand, within EX28DRAFT_4411 at 74.692 kb on - strand at 74.743 kb on - strand at 74.775 kb on - strand at 74.882 kb on + strand at 75.187 kb on - strand, within EX28DRAFT_4412 at 75.187 kb on - strand, within EX28DRAFT_4412 at 75.383 kb on + strand, within EX28DRAFT_4412 at 75.383 kb on + strand, within EX28DRAFT_4412 at 75.401 kb on + strand, within EX28DRAFT_4412
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 72,635 + +1.4 72,636 - -0.5 72,638 + +0.4 72,638 + -0.1 72,639 - +0.0 72,640 + -0.1 72,641 - -1.3 72,641 - +0.2 72,641 - -0.4 72,647 + +0.4 72,648 - +0.4 72,650 + -1.0 73,141 - EX28DRAFT_4410 0.24 +0.5 73,360 - +0.9 73,558 + -0.4 73,588 + -0.4 73,613 + -2.3 73,668 + EX28DRAFT_4411 0.14 -4.8 73,681 - EX28DRAFT_4411 0.15 -1.0 73,756 + EX28DRAFT_4411 0.20 -2.8 73,757 - EX28DRAFT_4411 0.20 -2.2 73,757 - EX28DRAFT_4411 0.20 -2.0 73,757 - EX28DRAFT_4411 0.20 -3.4 73,757 - EX28DRAFT_4411 0.20 -0.4 73,757 - EX28DRAFT_4411 0.20 -2.0 73,815 + EX28DRAFT_4411 0.25 -3.6 73,871 + EX28DRAFT_4411 0.29 -3.7 73,872 - EX28DRAFT_4411 0.29 -3.9 73,872 - EX28DRAFT_4411 0.29 -6.3 73,960 + EX28DRAFT_4411 0.36 -2.6 73,972 + EX28DRAFT_4411 0.36 -2.5 74,166 - EX28DRAFT_4411 0.51 -3.7 74,166 - EX28DRAFT_4411 0.51 -1.4 74,172 - EX28DRAFT_4411 0.51 -2.1 74,181 + EX28DRAFT_4411 0.52 -1.7 74,181 + EX28DRAFT_4411 0.52 -3.1 74,181 + EX28DRAFT_4411 0.52 -3.6 74,182 - EX28DRAFT_4411 0.52 -3.2 74,182 - EX28DRAFT_4411 0.52 -3.2 74,249 + EX28DRAFT_4411 0.57 -4.8 74,250 + EX28DRAFT_4411 0.57 -4.0 74,250 + EX28DRAFT_4411 0.57 -5.0 74,250 + EX28DRAFT_4411 0.57 -2.7 74,251 - EX28DRAFT_4411 0.57 -5.0 74,254 + EX28DRAFT_4411 0.58 -3.6 74,341 - EX28DRAFT_4411 0.64 -3.0 74,374 + EX28DRAFT_4411 0.67 -4.1 74,374 + EX28DRAFT_4411 0.67 -2.8 74,375 - EX28DRAFT_4411 0.67 -2.7 74,375 - EX28DRAFT_4411 0.67 -3.9 74,486 - EX28DRAFT_4411 0.75 -1.4 74,558 + EX28DRAFT_4411 0.80 -3.4 74,558 + EX28DRAFT_4411 0.80 -3.1 74,623 - EX28DRAFT_4411 0.85 -2.7 74,640 - EX28DRAFT_4411 0.86 -1.1 74,692 - -2.7 74,743 - -3.5 74,775 - -4.2 74,882 + -1.3 75,187 - EX28DRAFT_4412 0.15 +0.5 75,187 - EX28DRAFT_4412 0.15 +1.4 75,383 + EX28DRAFT_4412 0.23 -0.4 75,383 + EX28DRAFT_4412 0.23 -0.3 75,401 + EX28DRAFT_4412 0.24 +0.9
Or see this region's nucleotide sequence