Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4158 and EX28DRAFT_4159 are separated by 144 nucleotides EX28DRAFT_4159 and EX28DRAFT_4160 are separated by 142 nucleotides EX28DRAFT_4160 and EX28DRAFT_4161 are separated by 135 nucleotides EX28DRAFT_4161 and EX28DRAFT_4162 overlap by 4 nucleotides
EX28DRAFT_4158: EX28DRAFT_4158 - tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 138,753 to 139,757
_4158
EX28DRAFT_4159: EX28DRAFT_4159 - Protein of unknown function (DUF4223), at 139,902 to 140,072
_4159
EX28DRAFT_4160: EX28DRAFT_4160 - uroporphyrin-III C-methyltransferase, at 140,215 to 141,588
_4160
EX28DRAFT_4161: EX28DRAFT_4161 - nitrite reductase [NAD(P)H], small subunit, at 141,724 to 142,050
_4161
EX28DRAFT_4162: EX28DRAFT_4162 - nitrite reductase [NAD(P)H], large subunit, at 142,047 to 144,590
_4162
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-2
-1
0
1 at 139.777 kb on + strand at 139.777 kb on + strand at 139.778 kb on - strand at 139.778 kb on - strand at 139.778 kb on - strand at 139.903 kb on + strand at 139.969 kb on + strand, within EX28DRAFT_4159 at 139.970 kb on - strand, within EX28DRAFT_4159 at 139.975 kb on + strand, within EX28DRAFT_4159 at 139.976 kb on - strand, within EX28DRAFT_4159 at 139.976 kb on - strand, within EX28DRAFT_4159 at 139.976 kb on - strand, within EX28DRAFT_4159 at 139.986 kb on - strand, within EX28DRAFT_4159 at 140.002 kb on - strand, within EX28DRAFT_4159 at 140.088 kb on + strand at 140.106 kb on + strand at 140.148 kb on - strand at 140.209 kb on + strand at 140.210 kb on - strand at 140.210 kb on - strand at 140.303 kb on - strand at 140.303 kb on - strand at 140.447 kb on + strand, within EX28DRAFT_4160 at 140.448 kb on - strand, within EX28DRAFT_4160 at 140.448 kb on - strand, within EX28DRAFT_4160 at 140.599 kb on - strand, within EX28DRAFT_4160 at 140.850 kb on - strand, within EX28DRAFT_4160 at 141.414 kb on + strand, within EX28DRAFT_4160 at 141.595 kb on - strand at 141.695 kb on + strand at 141.696 kb on - strand at 141.696 kb on - strand at 141.725 kb on + strand at 141.767 kb on + strand, within EX28DRAFT_4161 at 141.767 kb on + strand, within EX28DRAFT_4161 at 141.768 kb on - strand, within EX28DRAFT_4161 at 141.919 kb on - strand, within EX28DRAFT_4161 at 142.033 kb on - strand at 142.079 kb on + strand at 142.079 kb on + strand at 142.079 kb on + strand at 142.088 kb on - strand at 142.131 kb on + strand at 142.131 kb on + strand at 142.132 kb on - strand at 142.132 kb on - strand at 142.304 kb on - strand, within EX28DRAFT_4162 at 142.372 kb on + strand, within EX28DRAFT_4162 at 142.372 kb on + strand, within EX28DRAFT_4162 at 142.478 kb on - strand, within EX28DRAFT_4162
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 139,777 + -0.5 139,777 + -0.3 139,778 - +0.1 139,778 - -1.0 139,778 - -0.2 139,903 + -0.1 139,969 + EX28DRAFT_4159 0.39 -0.3 139,970 - EX28DRAFT_4159 0.40 +0.3 139,975 + EX28DRAFT_4159 0.43 +0.5 139,976 - EX28DRAFT_4159 0.43 -0.2 139,976 - EX28DRAFT_4159 0.43 +0.6 139,976 - EX28DRAFT_4159 0.43 -0.1 139,986 - EX28DRAFT_4159 0.49 -0.2 140,002 - EX28DRAFT_4159 0.58 +1.0 140,088 + +0.0 140,106 + +0.9 140,148 - -0.6 140,209 + +0.2 140,210 - +0.5 140,210 - -0.1 140,303 - -0.0 140,303 - -1.2 140,447 + EX28DRAFT_4160 0.17 -0.2 140,448 - EX28DRAFT_4160 0.17 +0.5 140,448 - EX28DRAFT_4160 0.17 -1.9 140,599 - EX28DRAFT_4160 0.28 -0.3 140,850 - EX28DRAFT_4160 0.46 -0.7 141,414 + EX28DRAFT_4160 0.87 -1.2 141,595 - -2.1 141,695 + +0.9 141,696 - -0.3 141,696 - +0.2 141,725 + +0.3 141,767 + EX28DRAFT_4161 0.13 +0.6 141,767 + EX28DRAFT_4161 0.13 +0.0 141,768 - EX28DRAFT_4161 0.13 -0.6 141,919 - EX28DRAFT_4161 0.60 -0.1 142,033 - -0.1 142,079 + +0.1 142,079 + -0.5 142,079 + +0.6 142,088 - -0.1 142,131 + -0.1 142,131 + -0.1 142,132 - +0.7 142,132 - +0.4 142,304 - EX28DRAFT_4162 0.10 +0.2 142,372 + EX28DRAFT_4162 0.13 -1.1 142,372 + EX28DRAFT_4162 0.13 +0.4 142,478 - EX28DRAFT_4162 0.17 -0.0
Or see this region's nucleotide sequence