Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4136 and EX28DRAFT_4137 are separated by 88 nucleotides EX28DRAFT_4137 and EX28DRAFT_4138 are separated by 232 nucleotides EX28DRAFT_4138 and EX28DRAFT_4139 overlap by 4 nucleotides
EX28DRAFT_4136: EX28DRAFT_4136 - Transcriptional accessory protein, at 114,617 to 116,947
_4136
EX28DRAFT_4137: EX28DRAFT_4137 - transcription elongation factor GreB, at 117,036 to 117,509
_4137
EX28DRAFT_4138: EX28DRAFT_4138 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 117,742 to 118,461
_4138
EX28DRAFT_4139: EX28DRAFT_4139 - Signal transduction histidine kinase, at 118,458 to 119,804
_4139
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 116.785 kb on + strand at 116.886 kb on - strand at 116.908 kb on + strand at 116.920 kb on + strand at 116.984 kb on + strand at 116.985 kb on - strand at 116.985 kb on - strand at 116.985 kb on - strand at 116.989 kb on - strand at 116.989 kb on - strand at 116.989 kb on - strand at 117.042 kb on + strand at 117.043 kb on - strand at 117.064 kb on + strand at 117.065 kb on - strand at 117.065 kb on - strand at 117.160 kb on + strand, within EX28DRAFT_4137 at 117.160 kb on + strand, within EX28DRAFT_4137 at 117.161 kb on - strand, within EX28DRAFT_4137 at 117.161 kb on - strand, within EX28DRAFT_4137 at 117.362 kb on - strand, within EX28DRAFT_4137 at 117.367 kb on + strand, within EX28DRAFT_4137 at 117.535 kb on + strand at 117.553 kb on + strand at 117.553 kb on + strand at 117.582 kb on + strand at 117.583 kb on - strand at 117.593 kb on + strand at 117.593 kb on + strand at 117.614 kb on - strand at 117.625 kb on - strand at 117.638 kb on - strand at 117.668 kb on + strand at 117.669 kb on - strand at 117.681 kb on - strand at 117.700 kb on - strand at 117.700 kb on - strand at 117.809 kb on - strand at 117.936 kb on + strand, within EX28DRAFT_4138 at 118.046 kb on - strand, within EX28DRAFT_4138 at 118.321 kb on + strand, within EX28DRAFT_4138 at 118.402 kb on + strand at 118.402 kb on + strand at 118.404 kb on + strand at 118.404 kb on + strand at 118.405 kb on - strand at 118.405 kb on - strand at 118.405 kb on - strand at 118.484 kb on + strand at 118.549 kb on - strand at 118.549 kb on - strand at 118.595 kb on + strand, within EX28DRAFT_4139 at 118.596 kb on - strand, within EX28DRAFT_4139 at 118.596 kb on - strand, within EX28DRAFT_4139 at 118.598 kb on + strand, within EX28DRAFT_4139 at 118.598 kb on + strand, within EX28DRAFT_4139 at 118.598 kb on + strand, within EX28DRAFT_4139 at 118.598 kb on + strand, within EX28DRAFT_4139 at 118.599 kb on - strand, within EX28DRAFT_4139 at 118.622 kb on + strand, within EX28DRAFT_4139 at 118.623 kb on - strand, within EX28DRAFT_4139 at 118.987 kb on + strand, within EX28DRAFT_4139 at 118.988 kb on - strand, within EX28DRAFT_4139 at 118.997 kb on + strand, within EX28DRAFT_4139 at 118.999 kb on + strand, within EX28DRAFT_4139 at 118.999 kb on + strand, within EX28DRAFT_4139 at 118.999 kb on + strand, within EX28DRAFT_4139 at 119.000 kb on - strand, within EX28DRAFT_4139 at 119.057 kb on + strand, within EX28DRAFT_4139 at 119.117 kb on + strand, within EX28DRAFT_4139 at 119.123 kb on + strand, within EX28DRAFT_4139 at 119.124 kb on - strand, within EX28DRAFT_4139 at 119.160 kb on - strand, within EX28DRAFT_4139 at 119.211 kb on - strand, within EX28DRAFT_4139 at 119.251 kb on - strand, within EX28DRAFT_4139 at 119.270 kb on + strand, within EX28DRAFT_4139 at 119.289 kb on - strand, within EX28DRAFT_4139 at 119.446 kb on + strand, within EX28DRAFT_4139
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 116,785 + -0.4 116,886 - +0.6 116,908 + +0.3 116,920 + -0.3 116,984 + +0.0 116,985 - +0.1 116,985 - +1.2 116,985 - +0.3 116,989 - -0.8 116,989 - -0.5 116,989 - +0.5 117,042 + +0.6 117,043 - -1.3 117,064 + +0.3 117,065 - -0.1 117,065 - -0.1 117,160 + EX28DRAFT_4137 0.26 -0.8 117,160 + EX28DRAFT_4137 0.26 -0.5 117,161 - EX28DRAFT_4137 0.26 +0.8 117,161 - EX28DRAFT_4137 0.26 -1.0 117,362 - EX28DRAFT_4137 0.69 +0.5 117,367 + EX28DRAFT_4137 0.70 +2.7 117,535 + +0.8 117,553 + +0.4 117,553 + -0.2 117,582 + +0.2 117,583 - +0.8 117,593 + -0.1 117,593 + +0.1 117,614 - -0.6 117,625 - +1.1 117,638 - +0.1 117,668 + +0.7 117,669 - -0.1 117,681 - +0.2 117,700 - +0.7 117,700 - +0.9 117,809 - +2.3 117,936 + EX28DRAFT_4138 0.27 +0.7 118,046 - EX28DRAFT_4138 0.42 +0.0 118,321 + EX28DRAFT_4138 0.80 +0.9 118,402 + +0.0 118,402 + +2.2 118,404 + +0.4 118,404 + +0.3 118,405 - +0.3 118,405 - -0.0 118,405 - -2.1 118,484 + +0.7 118,549 - +0.4 118,549 - +0.3 118,595 + EX28DRAFT_4139 0.10 +0.7 118,596 - EX28DRAFT_4139 0.10 +0.4 118,596 - EX28DRAFT_4139 0.10 +0.4 118,598 + EX28DRAFT_4139 0.10 +0.2 118,598 + EX28DRAFT_4139 0.10 +0.7 118,598 + EX28DRAFT_4139 0.10 -1.8 118,598 + EX28DRAFT_4139 0.10 +0.7 118,599 - EX28DRAFT_4139 0.10 +0.3 118,622 + EX28DRAFT_4139 0.12 +0.0 118,623 - EX28DRAFT_4139 0.12 +0.0 118,987 + EX28DRAFT_4139 0.39 -0.6 118,988 - EX28DRAFT_4139 0.39 +0.8 118,997 + EX28DRAFT_4139 0.40 +1.5 118,999 + EX28DRAFT_4139 0.40 +0.5 118,999 + EX28DRAFT_4139 0.40 +0.9 118,999 + EX28DRAFT_4139 0.40 +0.2 119,000 - EX28DRAFT_4139 0.40 +0.1 119,057 + EX28DRAFT_4139 0.44 +0.5 119,117 + EX28DRAFT_4139 0.49 +0.5 119,123 + EX28DRAFT_4139 0.49 -0.2 119,124 - EX28DRAFT_4139 0.49 +0.2 119,160 - EX28DRAFT_4139 0.52 -0.6 119,211 - EX28DRAFT_4139 0.56 -0.3 119,251 - EX28DRAFT_4139 0.59 -0.0 119,270 + EX28DRAFT_4139 0.60 +0.1 119,289 - EX28DRAFT_4139 0.62 -0.0 119,446 + EX28DRAFT_4139 0.73 +0.2
Or see this region's nucleotide sequence