Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4104 and EX28DRAFT_4105 overlap by 4 nucleotides EX28DRAFT_4105 and EX28DRAFT_4106 are separated by 68 nucleotides EX28DRAFT_4106 and EX28DRAFT_4107 are separated by 69 nucleotides EX28DRAFT_4107 and EX28DRAFT_4108 are separated by 337 nucleotides
EX28DRAFT_4104: EX28DRAFT_4104 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 76,336 to 77,151
_4104
EX28DRAFT_4105: EX28DRAFT_4105 - Beta-ketoacyl synthase, N-terminal domain, at 77,148 to 77,870
_4105
EX28DRAFT_4106: EX28DRAFT_4106 - Methylase involved in ubiquinone/menaquinone biosynthesis, at 77,939 to 79,003
_4106
EX28DRAFT_4107: EX28DRAFT_4107 - hypothetical protein, at 79,073 to 79,462
_4107
EX28DRAFT_4108: EX28DRAFT_4108 - Predicted permease, at 79,800 to 80,849
_4108
Position (kb)
77
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 77.327 kb on - strand, within EX28DRAFT_4105 at 77.445 kb on + strand, within EX28DRAFT_4105 at 77.446 kb on - strand, within EX28DRAFT_4105 at 77.446 kb on - strand, within EX28DRAFT_4105 at 77.480 kb on + strand, within EX28DRAFT_4105 at 77.480 kb on + strand, within EX28DRAFT_4105 at 77.481 kb on - strand, within EX28DRAFT_4105 at 77.523 kb on - strand, within EX28DRAFT_4105 at 77.872 kb on + strand at 77.873 kb on - strand at 77.891 kb on + strand at 77.891 kb on + strand at 77.891 kb on + strand at 77.930 kb on + strand at 77.931 kb on - strand at 77.940 kb on + strand at 77.966 kb on + strand at 77.966 kb on + strand at 77.971 kb on + strand at 77.971 kb on + strand at 77.972 kb on - strand at 77.972 kb on - strand at 78.037 kb on - strand at 78.065 kb on - strand, within EX28DRAFT_4106 at 78.067 kb on + strand, within EX28DRAFT_4106 at 78.067 kb on + strand, within EX28DRAFT_4106 at 78.068 kb on - strand, within EX28DRAFT_4106 at 78.068 kb on - strand, within EX28DRAFT_4106 at 78.079 kb on + strand, within EX28DRAFT_4106 at 78.079 kb on + strand, within EX28DRAFT_4106 at 78.079 kb on + strand, within EX28DRAFT_4106 at 78.080 kb on - strand, within EX28DRAFT_4106 at 78.090 kb on + strand, within EX28DRAFT_4106 at 78.485 kb on + strand, within EX28DRAFT_4106 at 78.486 kb on - strand, within EX28DRAFT_4106 at 78.487 kb on + strand, within EX28DRAFT_4106 at 78.487 kb on + strand, within EX28DRAFT_4106 at 78.487 kb on + strand, within EX28DRAFT_4106 at 78.488 kb on - strand, within EX28DRAFT_4106 at 78.488 kb on - strand, within EX28DRAFT_4106 at 78.488 kb on - strand, within EX28DRAFT_4106 at 78.488 kb on - strand, within EX28DRAFT_4106 at 78.488 kb on - strand, within EX28DRAFT_4106 at 78.488 kb on - strand, within EX28DRAFT_4106 at 78.488 kb on - strand, within EX28DRAFT_4106 at 78.488 kb on - strand, within EX28DRAFT_4106 at 78.490 kb on - strand, within EX28DRAFT_4106 at 78.490 kb on - strand, within EX28DRAFT_4106 at 78.522 kb on + strand, within EX28DRAFT_4106 at 78.523 kb on - strand, within EX28DRAFT_4106 at 78.526 kb on - strand, within EX28DRAFT_4106 at 78.660 kb on - strand, within EX28DRAFT_4106 at 78.694 kb on + strand, within EX28DRAFT_4106 at 78.804 kb on - strand, within EX28DRAFT_4106 at 79.012 kb on - strand at 79.071 kb on + strand at 79.072 kb on - strand at 79.191 kb on + strand, within EX28DRAFT_4107 at 79.191 kb on + strand, within EX28DRAFT_4107 at 79.268 kb on - strand, within EX28DRAFT_4107 at 79.364 kb on + strand, within EX28DRAFT_4107 at 79.369 kb on + strand, within EX28DRAFT_4107 at 79.718 kb on + strand at 79.761 kb on - strand at 79.787 kb on - strand at 79.787 kb on - strand at 79.886 kb on + strand at 79.891 kb on - strand at 79.891 kb on - strand at 79.953 kb on + strand, within EX28DRAFT_4108 at 79.954 kb on - strand, within EX28DRAFT_4108 at 79.959 kb on + strand, within EX28DRAFT_4108 at 79.960 kb on - strand, within EX28DRAFT_4108 at 79.965 kb on + strand, within EX28DRAFT_4108 at 79.965 kb on + strand, within EX28DRAFT_4108 at 79.965 kb on + strand, within EX28DRAFT_4108
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 77,327 - EX28DRAFT_4105 0.25 +0.5 77,445 + EX28DRAFT_4105 0.41 +0.7 77,446 - EX28DRAFT_4105 0.41 -0.3 77,446 - EX28DRAFT_4105 0.41 -0.1 77,480 + EX28DRAFT_4105 0.46 +0.1 77,480 + EX28DRAFT_4105 0.46 +1.4 77,481 - EX28DRAFT_4105 0.46 -1.0 77,523 - EX28DRAFT_4105 0.52 +0.1 77,872 + +0.0 77,873 - -1.0 77,891 + -0.7 77,891 + +0.2 77,891 + +0.2 77,930 + +0.6 77,931 - -0.4 77,940 + +0.6 77,966 + +0.0 77,966 + -0.3 77,971 + -0.0 77,971 + +0.0 77,972 - +1.5 77,972 - +0.1 78,037 - +0.6 78,065 - EX28DRAFT_4106 0.12 +0.1 78,067 + EX28DRAFT_4106 0.12 +0.4 78,067 + EX28DRAFT_4106 0.12 -0.6 78,068 - EX28DRAFT_4106 0.12 -0.7 78,068 - EX28DRAFT_4106 0.12 -0.4 78,079 + EX28DRAFT_4106 0.13 +0.2 78,079 + EX28DRAFT_4106 0.13 -0.5 78,079 + EX28DRAFT_4106 0.13 -0.1 78,080 - EX28DRAFT_4106 0.13 +0.1 78,090 + EX28DRAFT_4106 0.14 +0.2 78,485 + EX28DRAFT_4106 0.51 -1.8 78,486 - EX28DRAFT_4106 0.51 -0.1 78,487 + EX28DRAFT_4106 0.51 -0.3 78,487 + EX28DRAFT_4106 0.51 -0.3 78,487 + EX28DRAFT_4106 0.51 +0.9 78,488 - EX28DRAFT_4106 0.52 +0.1 78,488 - EX28DRAFT_4106 0.52 +0.1 78,488 - EX28DRAFT_4106 0.52 +0.7 78,488 - EX28DRAFT_4106 0.52 +0.1 78,488 - EX28DRAFT_4106 0.52 -1.6 78,488 - EX28DRAFT_4106 0.52 -0.7 78,488 - EX28DRAFT_4106 0.52 -0.6 78,488 - EX28DRAFT_4106 0.52 +0.3 78,490 - EX28DRAFT_4106 0.52 -0.1 78,490 - EX28DRAFT_4106 0.52 +0.1 78,522 + EX28DRAFT_4106 0.55 +0.4 78,523 - EX28DRAFT_4106 0.55 -0.9 78,526 - EX28DRAFT_4106 0.55 +0.0 78,660 - EX28DRAFT_4106 0.68 -1.5 78,694 + EX28DRAFT_4106 0.71 +0.2 78,804 - EX28DRAFT_4106 0.81 +1.3 79,012 - +0.2 79,071 + +0.9 79,072 - -0.6 79,191 + EX28DRAFT_4107 0.30 +0.5 79,191 + EX28DRAFT_4107 0.30 -0.7 79,268 - EX28DRAFT_4107 0.50 +0.2 79,364 + EX28DRAFT_4107 0.75 +0.1 79,369 + EX28DRAFT_4107 0.76 +0.3 79,718 + -0.4 79,761 - +0.5 79,787 - -0.5 79,787 - +0.1 79,886 + -0.5 79,891 - +2.1 79,891 - +0.6 79,953 + EX28DRAFT_4108 0.15 +0.5 79,954 - EX28DRAFT_4108 0.15 -0.1 79,959 + EX28DRAFT_4108 0.15 +0.2 79,960 - EX28DRAFT_4108 0.15 +0.2 79,965 + EX28DRAFT_4108 0.16 +3.5 79,965 + EX28DRAFT_4108 0.16 -1.7 79,965 + EX28DRAFT_4108 0.16 +0.1
Or see this region's nucleotide sequence