Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4063 and EX28DRAFT_4064 overlap by 35 nucleotides EX28DRAFT_4064 and EX28DRAFT_4065 are separated by 96 nucleotides EX28DRAFT_4065 and EX28DRAFT_4066 are separated by 283 nucleotides EX28DRAFT_4066 and EX28DRAFT_4067 are separated by 43 nucleotides
EX28DRAFT_4063: EX28DRAFT_4063 - Zn-dependent oligopeptidases, at 38,445 to 40,487
_4063
EX28DRAFT_4064: EX28DRAFT_4064 - Putative SAM-dependent methyltransferase, at 40,453 to 41,247
_4064
EX28DRAFT_4065: EX28DRAFT_4065 - Universal stress protein UspA and related nucleotide-binding proteins, at 41,344 to 41,781
_4065
EX28DRAFT_4066: EX28DRAFT_4066 - Universal stress protein B (UspB), at 42,065 to 42,400
_4066
EX28DRAFT_4067: EX28DRAFT_4067 - hypothetical protein, at 42,444 to 42,851
_4067
Position (kb) 41
42 Strain fitness (log2 ratio) -2
-1
0
1 at 40.493 kb on - strand at 40.508 kb on + strand at 40.508 kb on + strand at 40.508 kb on + strand at 40.509 kb on - strand at 40.512 kb on - strand at 40.756 kb on + strand, within EX28DRAFT_4064 at 41.000 kb on - strand, within EX28DRAFT_4064 at 41.000 kb on - strand, within EX28DRAFT_4064 at 41.011 kb on - strand, within EX28DRAFT_4064 at 41.011 kb on - strand, within EX28DRAFT_4064 at 41.022 kb on + strand, within EX28DRAFT_4064 at 41.158 kb on + strand, within EX28DRAFT_4064 at 41.294 kb on + strand at 41.294 kb on + strand at 41.295 kb on - strand at 41.337 kb on - strand at 41.345 kb on + strand at 41.345 kb on + strand at 41.345 kb on + strand at 41.346 kb on - strand at 41.478 kb on - strand, within EX28DRAFT_4065 at 41.655 kb on - strand, within EX28DRAFT_4065 at 41.719 kb on - strand, within EX28DRAFT_4065 at 41.782 kb on - strand at 41.782 kb on - strand at 41.798 kb on + strand at 41.798 kb on + strand at 41.799 kb on - strand at 41.799 kb on - strand at 41.814 kb on - strand at 41.856 kb on + strand at 41.857 kb on - strand at 41.862 kb on + strand at 41.863 kb on - strand at 41.950 kb on - strand at 41.953 kb on - strand at 42.064 kb on + strand at 42.064 kb on + strand at 42.064 kb on + strand at 42.064 kb on + strand at 42.064 kb on + strand at 42.065 kb on - strand at 42.065 kb on - strand at 42.112 kb on - strand, within EX28DRAFT_4066 at 42.128 kb on + strand, within EX28DRAFT_4066 at 42.130 kb on + strand, within EX28DRAFT_4066 at 42.160 kb on - strand, within EX28DRAFT_4066 at 42.191 kb on + strand, within EX28DRAFT_4066 at 42.257 kb on + strand, within EX28DRAFT_4066 at 42.258 kb on - strand, within EX28DRAFT_4066 at 42.263 kb on + strand, within EX28DRAFT_4066 at 42.264 kb on - strand, within EX28DRAFT_4066 at 42.264 kb on - strand, within EX28DRAFT_4066 at 42.267 kb on - strand, within EX28DRAFT_4066 at 42.301 kb on + strand, within EX28DRAFT_4066 at 42.302 kb on - strand, within EX28DRAFT_4066 at 42.476 kb on + strand at 42.476 kb on + strand at 42.476 kb on + strand at 42.477 kb on - strand at 42.509 kb on - strand, within EX28DRAFT_4067 at 42.534 kb on - strand, within EX28DRAFT_4067 at 42.534 kb on - strand, within EX28DRAFT_4067 at 42.605 kb on + strand, within EX28DRAFT_4067 at 42.605 kb on + strand, within EX28DRAFT_4067 at 42.606 kb on - strand, within EX28DRAFT_4067 at 42.606 kb on - strand, within EX28DRAFT_4067 at 42.606 kb on - strand, within EX28DRAFT_4067 at 42.640 kb on + strand, within EX28DRAFT_4067 at 42.640 kb on + strand, within EX28DRAFT_4067 at 42.641 kb on - strand, within EX28DRAFT_4067 at 42.641 kb on - strand, within EX28DRAFT_4067 at 42.641 kb on - strand, within EX28DRAFT_4067 at 42.650 kb on - strand, within EX28DRAFT_4067 at 42.692 kb on + strand, within EX28DRAFT_4067 at 42.692 kb on + strand, within EX28DRAFT_4067 at 42.693 kb on - strand, within EX28DRAFT_4067 at 42.694 kb on + strand, within EX28DRAFT_4067 at 42.694 kb on + strand, within EX28DRAFT_4067 at 42.732 kb on + strand, within EX28DRAFT_4067 at 42.732 kb on + strand, within EX28DRAFT_4067
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 40,493 - -1.2 40,508 + -0.1 40,508 + +1.0 40,508 + +0.5 40,509 - -0.4 40,512 - -0.3 40,756 + EX28DRAFT_4064 0.38 -0.5 41,000 - EX28DRAFT_4064 0.69 -0.1 41,000 - EX28DRAFT_4064 0.69 -0.2 41,011 - EX28DRAFT_4064 0.70 -0.2 41,011 - EX28DRAFT_4064 0.70 -1.7 41,022 + EX28DRAFT_4064 0.72 -0.6 41,158 + EX28DRAFT_4064 0.89 +0.5 41,294 + -1.1 41,294 + -0.1 41,295 - +1.1 41,337 - +0.1 41,345 + +0.3 41,345 + +0.6 41,345 + -1.6 41,346 - +0.2 41,478 - EX28DRAFT_4065 0.31 +0.6 41,655 - EX28DRAFT_4065 0.71 +0.8 41,719 - EX28DRAFT_4065 0.86 +0.8 41,782 - +0.6 41,782 - -0.6 41,798 + -1.2 41,798 + +0.5 41,799 - +0.4 41,799 - -0.9 41,814 - -1.1 41,856 + -1.8 41,857 - -1.4 41,862 + +0.2 41,863 - -0.4 41,950 - +1.7 41,953 - +0.3 42,064 + +0.1 42,064 + -0.6 42,064 + +1.2 42,064 + +0.1 42,064 + -0.6 42,065 - -0.7 42,065 - -0.1 42,112 - EX28DRAFT_4066 0.14 -0.3 42,128 + EX28DRAFT_4066 0.19 +0.3 42,130 + EX28DRAFT_4066 0.19 -0.1 42,160 - EX28DRAFT_4066 0.28 +0.1 42,191 + EX28DRAFT_4066 0.38 +0.3 42,257 + EX28DRAFT_4066 0.57 +0.1 42,258 - EX28DRAFT_4066 0.57 -1.5 42,263 + EX28DRAFT_4066 0.59 -0.1 42,264 - EX28DRAFT_4066 0.59 -0.7 42,264 - EX28DRAFT_4066 0.59 +0.1 42,267 - EX28DRAFT_4066 0.60 -1.3 42,301 + EX28DRAFT_4066 0.70 +0.1 42,302 - EX28DRAFT_4066 0.71 -0.5 42,476 + +0.4 42,476 + -0.5 42,476 + -0.0 42,477 - +0.7 42,509 - EX28DRAFT_4067 0.16 +0.2 42,534 - EX28DRAFT_4067 0.22 +0.3 42,534 - EX28DRAFT_4067 0.22 -0.1 42,605 + EX28DRAFT_4067 0.39 -0.2 42,605 + EX28DRAFT_4067 0.39 +0.5 42,606 - EX28DRAFT_4067 0.40 +0.2 42,606 - EX28DRAFT_4067 0.40 -0.2 42,606 - EX28DRAFT_4067 0.40 -0.9 42,640 + EX28DRAFT_4067 0.48 -0.3 42,640 + EX28DRAFT_4067 0.48 -0.3 42,641 - EX28DRAFT_4067 0.48 -0.1 42,641 - EX28DRAFT_4067 0.48 +1.2 42,641 - EX28DRAFT_4067 0.48 +0.4 42,650 - EX28DRAFT_4067 0.50 -0.2 42,692 + EX28DRAFT_4067 0.61 +0.1 42,692 + EX28DRAFT_4067 0.61 -0.1 42,693 - EX28DRAFT_4067 0.61 -0.5 42,694 + EX28DRAFT_4067 0.61 -0.1 42,694 + EX28DRAFT_4067 0.61 -0.4 42,732 + EX28DRAFT_4067 0.71 -0.5 42,732 + EX28DRAFT_4067 0.71 +0.5
Or see this region's nucleotide sequence