Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3574 and EX28DRAFT_3575 are separated by 41 nucleotides EX28DRAFT_3575 and EX28DRAFT_3576 are separated by 33 nucleotides EX28DRAFT_3576 and EX28DRAFT_3577 are separated by 33 nucleotides EX28DRAFT_3577 and EX28DRAFT_3578 are separated by 28 nucleotides EX28DRAFT_3578 and EX28DRAFT_3579 are separated by 172 nucleotides EX28DRAFT_3579 and EX28DRAFT_3580 are separated by 102 nucleotides EX28DRAFT_3580 and EX28DRAFT_3581 overlap by 32 nucleotides EX28DRAFT_3581 and EX28DRAFT_3582 are separated by 20 nucleotides
EX28DRAFT_3574: EX28DRAFT_3574 - Protein of unknown function (DUF1435), at 236,283 to 236,513
_3574
EX28DRAFT_3575: EX28DRAFT_3575 - tRNA, at 236,555 to 236,640
_3575
EX28DRAFT_3576: EX28DRAFT_3576 - tRNA, at 236,674 to 236,759
_3576
EX28DRAFT_3577: EX28DRAFT_3577 - tRNA, at 236,793 to 236,879
_3577
EX28DRAFT_3578: EX28DRAFT_3578 - tRNA, at 236,908 to 236,994
_3578
EX28DRAFT_3579: EX28DRAFT_3579 - 16S RNA G1207 methylase RsmC, at 237,167 to 238,195
_3579
EX28DRAFT_3580: EX28DRAFT_3580 - DNA polymerase III, psi subunit (EC 2.7.7.7), at 238,298 to 238,711
_3580
EX28DRAFT_3581: EX28DRAFT_3581 - ribosomal-protein-alanine acetyltransferase, at 238,680 to 239,123
_3581
EX28DRAFT_3582: EX28DRAFT_3582 - noncanonical pyrimidine nucleotidase, YjjG family, at 239,144 to 239,821
_3582
Position (kb) 237
238
239 Strain fitness (log2 ratio) -2
-1
0
1 at 236.467 kb on + strand, within EX28DRAFT_3574 at 236.546 kb on + strand at 236.547 kb on - strand at 236.552 kb on - strand at 236.552 kb on - strand at 236.552 kb on - strand at 236.599 kb on - strand, within EX28DRAFT_3575 at 236.851 kb on - strand, within EX28DRAFT_3577 at 236.894 kb on - strand at 236.894 kb on - strand at 236.907 kb on - strand at 236.907 kb on - strand at 237.017 kb on - strand at 237.168 kb on + strand at 237.316 kb on + strand, within EX28DRAFT_3579 at 237.400 kb on + strand, within EX28DRAFT_3579 at 237.401 kb on - strand, within EX28DRAFT_3579 at 237.497 kb on + strand, within EX28DRAFT_3579 at 237.724 kb on - strand, within EX28DRAFT_3579 at 237.768 kb on + strand, within EX28DRAFT_3579 at 237.769 kb on - strand, within EX28DRAFT_3579 at 237.949 kb on - strand, within EX28DRAFT_3579 at 237.951 kb on + strand, within EX28DRAFT_3579 at 237.951 kb on + strand, within EX28DRAFT_3579 at 238.051 kb on - strand, within EX28DRAFT_3579 at 238.053 kb on + strand, within EX28DRAFT_3579 at 238.054 kb on - strand, within EX28DRAFT_3579 at 238.111 kb on + strand at 238.112 kb on - strand at 238.229 kb on + strand at 238.229 kb on + strand at 238.229 kb on + strand at 238.230 kb on - strand at 238.230 kb on - strand at 238.230 kb on - strand at 238.230 kb on - strand at 238.230 kb on - strand at 238.232 kb on + strand at 238.236 kb on + strand at 238.237 kb on - strand at 238.237 kb on - strand at 238.881 kb on - strand, within EX28DRAFT_3581 at 239.060 kb on + strand, within EX28DRAFT_3581 at 239.065 kb on - strand, within EX28DRAFT_3581 at 239.065 kb on - strand, within EX28DRAFT_3581 at 239.131 kb on + strand at 239.132 kb on - strand at 239.188 kb on + strand at 239.189 kb on - strand at 239.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 236,467 + EX28DRAFT_3574 0.80 -0.2 236,546 + +1.1 236,547 - -0.1 236,552 - +0.1 236,552 - +0.1 236,552 - +0.2 236,599 - EX28DRAFT_3575 0.51 -2.3 236,851 - EX28DRAFT_3577 0.67 -0.4 236,894 - -2.3 236,894 - +0.7 236,907 - +0.4 236,907 - -0.3 237,017 - -0.4 237,168 + -1.0 237,316 + EX28DRAFT_3579 0.14 -0.1 237,400 + EX28DRAFT_3579 0.23 -0.9 237,401 - EX28DRAFT_3579 0.23 +0.9 237,497 + EX28DRAFT_3579 0.32 -0.5 237,724 - EX28DRAFT_3579 0.54 +0.9 237,768 + EX28DRAFT_3579 0.58 +0.7 237,769 - EX28DRAFT_3579 0.59 -1.2 237,949 - EX28DRAFT_3579 0.76 -0.6 237,951 + EX28DRAFT_3579 0.76 -1.3 237,951 + EX28DRAFT_3579 0.76 -0.6 238,051 - EX28DRAFT_3579 0.86 +0.1 238,053 + EX28DRAFT_3579 0.86 -1.0 238,054 - EX28DRAFT_3579 0.86 +0.1 238,111 + -0.4 238,112 - +0.2 238,229 + -0.4 238,229 + +0.2 238,229 + -0.3 238,230 - -1.0 238,230 - +0.0 238,230 - +0.4 238,230 - +0.7 238,230 - +1.5 238,232 + +0.4 238,236 + -0.5 238,237 - +0.5 238,237 - -0.0 238,881 - EX28DRAFT_3581 0.45 +0.3 239,060 + EX28DRAFT_3581 0.86 -0.0 239,065 - EX28DRAFT_3581 0.87 +0.4 239,065 - EX28DRAFT_3581 0.87 -0.2 239,131 + +0.2 239,132 - +0.6 239,188 + -0.2 239,189 - +0.5 239,189 - +0.2
Or see this region's nucleotide sequence