Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1883 and EX28DRAFT_1884 are separated by 33 nucleotides EX28DRAFT_1884 and EX28DRAFT_1885 are separated by 106 nucleotides EX28DRAFT_1885 and EX28DRAFT_1886 are separated by 164 nucleotides EX28DRAFT_1886 and EX28DRAFT_1887 are separated by 61 nucleotides
EX28DRAFT_1883: EX28DRAFT_1883 - Acyltransferase family, at 1,896,389 to 1,897,528
_1883
EX28DRAFT_1884: EX28DRAFT_1884 - Predicted phosphatase homologous to the C-terminal domain of histone macroH2A1, at 1,897,562 to 1,898,104
_1884
EX28DRAFT_1885: EX28DRAFT_1885 - hypothetical protein, at 1,898,211 to 1,898,531
_1885
EX28DRAFT_1886: EX28DRAFT_1886 - Thin aggregative fimbriae synthesis protein, at 1,898,696 to 1,899,028
_1886
EX28DRAFT_1887: EX28DRAFT_1887 - Curlin associated repeat, at 1,899,090 to 1,899,542
_1887
Position (kb)
1898
1899 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1897.228 kb on + strand, within EX28DRAFT_1883 at 1897.310 kb on + strand, within EX28DRAFT_1883 at 1897.310 kb on + strand, within EX28DRAFT_1883 at 1897.311 kb on - strand, within EX28DRAFT_1883 at 1897.311 kb on - strand, within EX28DRAFT_1883 at 1897.333 kb on + strand, within EX28DRAFT_1883 at 1897.333 kb on + strand, within EX28DRAFT_1883 at 1897.337 kb on + strand, within EX28DRAFT_1883 at 1897.376 kb on + strand, within EX28DRAFT_1883 at 1897.376 kb on + strand, within EX28DRAFT_1883 at 1897.377 kb on - strand, within EX28DRAFT_1883 at 1897.438 kb on - strand at 1897.438 kb on - strand at 1897.438 kb on - strand at 1897.452 kb on + strand at 1897.526 kb on + strand at 1897.526 kb on + strand at 1897.675 kb on - strand, within EX28DRAFT_1884 at 1897.717 kb on + strand, within EX28DRAFT_1884 at 1897.718 kb on - strand, within EX28DRAFT_1884 at 1897.765 kb on + strand, within EX28DRAFT_1884 at 1897.872 kb on - strand, within EX28DRAFT_1884 at 1898.060 kb on + strand at 1898.060 kb on + strand at 1898.061 kb on - strand at 1898.069 kb on - strand at 1898.084 kb on - strand at 1898.084 kb on - strand at 1898.104 kb on + strand at 1898.134 kb on - strand at 1898.139 kb on + strand at 1898.260 kb on + strand, within EX28DRAFT_1885 at 1898.261 kb on - strand, within EX28DRAFT_1885 at 1898.261 kb on - strand, within EX28DRAFT_1885 at 1898.268 kb on - strand, within EX28DRAFT_1885 at 1898.320 kb on + strand, within EX28DRAFT_1885 at 1898.321 kb on - strand, within EX28DRAFT_1885 at 1898.342 kb on + strand, within EX28DRAFT_1885 at 1898.522 kb on - strand at 1898.522 kb on - strand at 1898.534 kb on + strand at 1898.538 kb on + strand at 1898.538 kb on + strand at 1898.539 kb on - strand at 1898.643 kb on + strand at 1898.643 kb on + strand at 1898.643 kb on + strand at 1898.644 kb on - strand at 1898.644 kb on - strand at 1898.644 kb on - strand at 1898.644 kb on - strand at 1898.795 kb on - strand, within EX28DRAFT_1886 at 1898.813 kb on + strand, within EX28DRAFT_1886 at 1898.813 kb on + strand, within EX28DRAFT_1886 at 1898.814 kb on - strand, within EX28DRAFT_1886 at 1898.839 kb on + strand, within EX28DRAFT_1886 at 1898.840 kb on - strand, within EX28DRAFT_1886 at 1898.892 kb on - strand, within EX28DRAFT_1886 at 1898.899 kb on + strand, within EX28DRAFT_1886 at 1898.947 kb on + strand, within EX28DRAFT_1886 at 1898.947 kb on + strand, within EX28DRAFT_1886 at 1898.947 kb on + strand, within EX28DRAFT_1886 at 1898.947 kb on + strand, within EX28DRAFT_1886 at 1898.947 kb on + strand, within EX28DRAFT_1886 at 1898.948 kb on - strand, within EX28DRAFT_1886 at 1898.959 kb on + strand, within EX28DRAFT_1886 at 1899.017 kb on + strand at 1899.023 kb on - strand at 1899.074 kb on - strand at 1899.095 kb on - strand at 1899.408 kb on - strand, within EX28DRAFT_1887 at 1899.476 kb on + strand, within EX28DRAFT_1887 at 1899.476 kb on + strand, within EX28DRAFT_1887 at 1899.489 kb on - strand, within EX28DRAFT_1887
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,897,228 + EX28DRAFT_1883 0.74 +1.6 1,897,310 + EX28DRAFT_1883 0.81 -1.2 1,897,310 + EX28DRAFT_1883 0.81 +0.2 1,897,311 - EX28DRAFT_1883 0.81 -0.5 1,897,311 - EX28DRAFT_1883 0.81 +0.6 1,897,333 + EX28DRAFT_1883 0.83 -0.2 1,897,333 + EX28DRAFT_1883 0.83 +0.0 1,897,337 + EX28DRAFT_1883 0.83 +0.5 1,897,376 + EX28DRAFT_1883 0.87 -0.8 1,897,376 + EX28DRAFT_1883 0.87 +0.2 1,897,377 - EX28DRAFT_1883 0.87 -0.5 1,897,438 - -1.3 1,897,438 - -0.2 1,897,438 - -0.6 1,897,452 + -0.2 1,897,526 + +1.3 1,897,526 + +0.2 1,897,675 - EX28DRAFT_1884 0.21 -1.0 1,897,717 + EX28DRAFT_1884 0.29 -1.4 1,897,718 - EX28DRAFT_1884 0.29 +0.5 1,897,765 + EX28DRAFT_1884 0.37 -1.0 1,897,872 - EX28DRAFT_1884 0.57 -0.3 1,898,060 + -1.9 1,898,060 + -0.5 1,898,061 - +0.4 1,898,069 - -0.4 1,898,084 - +0.6 1,898,084 - -0.0 1,898,104 + -3.0 1,898,134 - +0.1 1,898,139 + +1.2 1,898,260 + EX28DRAFT_1885 0.15 +0.6 1,898,261 - EX28DRAFT_1885 0.16 +0.9 1,898,261 - EX28DRAFT_1885 0.16 +0.4 1,898,268 - EX28DRAFT_1885 0.18 -2.3 1,898,320 + EX28DRAFT_1885 0.34 +0.7 1,898,321 - EX28DRAFT_1885 0.34 +1.2 1,898,342 + EX28DRAFT_1885 0.41 +0.1 1,898,522 - +0.5 1,898,522 - +0.7 1,898,534 + +0.7 1,898,538 + +0.3 1,898,538 + +0.5 1,898,539 - -0.9 1,898,643 + -0.1 1,898,643 + +0.3 1,898,643 + +3.2 1,898,644 - -0.8 1,898,644 - +1.4 1,898,644 - -0.6 1,898,644 - +0.4 1,898,795 - EX28DRAFT_1886 0.30 -0.6 1,898,813 + EX28DRAFT_1886 0.35 +0.0 1,898,813 + EX28DRAFT_1886 0.35 +0.4 1,898,814 - EX28DRAFT_1886 0.35 +0.6 1,898,839 + EX28DRAFT_1886 0.43 -1.7 1,898,840 - EX28DRAFT_1886 0.43 +0.2 1,898,892 - EX28DRAFT_1886 0.59 +0.6 1,898,899 + EX28DRAFT_1886 0.61 +1.0 1,898,947 + EX28DRAFT_1886 0.75 -0.0 1,898,947 + EX28DRAFT_1886 0.75 +0.9 1,898,947 + EX28DRAFT_1886 0.75 -0.3 1,898,947 + EX28DRAFT_1886 0.75 -0.6 1,898,947 + EX28DRAFT_1886 0.75 -0.3 1,898,948 - EX28DRAFT_1886 0.76 -1.6 1,898,959 + EX28DRAFT_1886 0.79 +0.2 1,899,017 + +1.3 1,899,023 - -0.6 1,899,074 - +0.8 1,899,095 - -0.5 1,899,408 - EX28DRAFT_1887 0.70 +0.0 1,899,476 + EX28DRAFT_1887 0.85 +0.1 1,899,476 + EX28DRAFT_1887 0.85 +0.3 1,899,489 - EX28DRAFT_1887 0.88 +0.7
Or see this region's nucleotide sequence