Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1658 and EX28DRAFT_1659 are separated by 30 nucleotides EX28DRAFT_1659 and EX28DRAFT_1660 are separated by 237 nucleotides EX28DRAFT_1660 and EX28DRAFT_1661 are separated by 36 nucleotides EX28DRAFT_1661 and EX28DRAFT_1662 are separated by 3 nucleotides EX28DRAFT_1662 and EX28DRAFT_1663 are separated by 4 nucleotides EX28DRAFT_1663 and EX28DRAFT_1664 are separated by 118 nucleotides
EX28DRAFT_1658: EX28DRAFT_1658 - Cyclopropane fatty acid synthase and related methyltransferases, at 1,674,690 to 1,675,838
_1658
EX28DRAFT_1659: EX28DRAFT_1659 - riboflavin synthase alpha chain (EC 2.5.1.9), at 1,675,869 to 1,676,513
_1659
EX28DRAFT_1660: EX28DRAFT_1660 - putative efflux protein, MATE family, at 1,676,751 to 1,678,112
_1660
EX28DRAFT_1661: EX28DRAFT_1661 - hypothetical protein, at 1,678,149 to 1,678,262
_1661
EX28DRAFT_1662: EX28DRAFT_1662 - tRNA, at 1,678,266 to 1,678,342
_1662
EX28DRAFT_1663: EX28DRAFT_1663 - tRNA, at 1,678,347 to 1,678,423
_1663
EX28DRAFT_1664: EX28DRAFT_1664 - Uncharacterized protein conserved in bacteria, at 1,678,542 to 1,680,137
_1664
Position (kb)
1676
1677
1678
1679 Strain fitness (log2 ratio)
-1
0
1
2 at 1675.795 kb on + strand at 1675.796 kb on - strand at 1675.822 kb on + strand at 1675.863 kb on + strand at 1675.896 kb on + strand at 1676.628 kb on + strand at 1676.628 kb on + strand at 1676.629 kb on - strand at 1676.629 kb on - strand at 1676.629 kb on - strand at 1676.629 kb on - strand at 1676.632 kb on + strand at 1676.647 kb on - strand at 1676.647 kb on - strand at 1676.651 kb on + strand at 1676.652 kb on - strand at 1676.716 kb on - strand at 1676.726 kb on + strand at 1676.727 kb on - strand at 1676.770 kb on + strand at 1676.771 kb on - strand at 1676.783 kb on + strand at 1676.784 kb on - strand at 1677.089 kb on + strand, within EX28DRAFT_1660 at 1677.090 kb on - strand, within EX28DRAFT_1660 at 1677.113 kb on + strand, within EX28DRAFT_1660 at 1677.113 kb on + strand, within EX28DRAFT_1660 at 1677.114 kb on - strand, within EX28DRAFT_1660 at 1677.460 kb on + strand, within EX28DRAFT_1660 at 1677.460 kb on + strand, within EX28DRAFT_1660 at 1677.461 kb on - strand, within EX28DRAFT_1660 at 1677.868 kb on + strand, within EX28DRAFT_1660 at 1677.881 kb on + strand, within EX28DRAFT_1660 at 1677.906 kb on - strand, within EX28DRAFT_1660 at 1677.949 kb on - strand, within EX28DRAFT_1660 at 1678.041 kb on - strand at 1678.482 kb on - strand at 1678.584 kb on + strand at 1678.585 kb on - strand at 1678.587 kb on - strand at 1678.671 kb on + strand at 1678.671 kb on + strand at 1678.672 kb on - strand at 1678.685 kb on + strand at 1678.746 kb on + strand, within EX28DRAFT_1664 at 1678.746 kb on + strand, within EX28DRAFT_1664 at 1678.883 kb on - strand, within EX28DRAFT_1664
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,675,795 + +0.4 1,675,796 - +0.5 1,675,822 + -0.3 1,675,863 + -0.1 1,675,896 + -0.6 1,676,628 + +0.8 1,676,628 + -0.5 1,676,629 - -1.4 1,676,629 - +0.6 1,676,629 - +0.1 1,676,629 - -0.0 1,676,632 + -0.5 1,676,647 - -0.1 1,676,647 - -0.2 1,676,651 + -0.1 1,676,652 - +0.4 1,676,716 - -0.6 1,676,726 + -0.9 1,676,727 - -0.6 1,676,770 + +1.3 1,676,771 - -0.3 1,676,783 + -1.5 1,676,784 - -0.0 1,677,089 + EX28DRAFT_1660 0.25 +0.2 1,677,090 - EX28DRAFT_1660 0.25 -0.1 1,677,113 + EX28DRAFT_1660 0.27 +0.3 1,677,113 + EX28DRAFT_1660 0.27 +0.4 1,677,114 - EX28DRAFT_1660 0.27 +0.1 1,677,460 + EX28DRAFT_1660 0.52 +0.3 1,677,460 + EX28DRAFT_1660 0.52 +0.7 1,677,461 - EX28DRAFT_1660 0.52 -1.6 1,677,868 + EX28DRAFT_1660 0.82 +0.7 1,677,881 + EX28DRAFT_1660 0.83 -0.4 1,677,906 - EX28DRAFT_1660 0.85 -0.0 1,677,949 - EX28DRAFT_1660 0.88 +0.2 1,678,041 - -0.6 1,678,482 - +0.9 1,678,584 + +0.9 1,678,585 - +2.3 1,678,587 - -0.0 1,678,671 + -0.8 1,678,671 + +0.8 1,678,672 - +0.1 1,678,685 + +0.0 1,678,746 + EX28DRAFT_1664 0.13 +0.7 1,678,746 + EX28DRAFT_1664 0.13 -1.0 1,678,883 - EX28DRAFT_1664 0.21 +0.7
Or see this region's nucleotide sequence