Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1383 and EX28DRAFT_1384 are separated by 39 nucleotides EX28DRAFT_1384 and EX28DRAFT_1385 are separated by 64 nucleotides EX28DRAFT_1385 and EX28DRAFT_1386 overlap by 4 nucleotides
EX28DRAFT_1383: EX28DRAFT_1383 - Uncharacterized protein conserved in bacteria, at 1,395,179 to 1,395,646
_1383
EX28DRAFT_1384: EX28DRAFT_1384 - Predicted ester cyclase, at 1,395,686 to 1,396,225
_1384
EX28DRAFT_1385: EX28DRAFT_1385 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,396,290 to 1,397,009
_1385
EX28DRAFT_1386: EX28DRAFT_1386 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 1,397,006 to 1,398,121
_1386
Position (kb)
1396
1397
1398 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1395.450 kb on - strand, within EX28DRAFT_1383 at 1395.556 kb on + strand, within EX28DRAFT_1383 at 1395.644 kb on + strand at 1395.839 kb on - strand, within EX28DRAFT_1384 at 1395.947 kb on - strand, within EX28DRAFT_1384 at 1396.115 kb on + strand, within EX28DRAFT_1384 at 1396.117 kb on + strand, within EX28DRAFT_1384 at 1396.188 kb on - strand at 1396.210 kb on - strand at 1396.217 kb on - strand at 1396.239 kb on - strand at 1396.243 kb on + strand at 1396.244 kb on - strand at 1396.246 kb on + strand at 1396.247 kb on - strand at 1396.247 kb on - strand at 1396.247 kb on - strand at 1396.316 kb on + strand at 1396.466 kb on - strand, within EX28DRAFT_1385 at 1396.661 kb on - strand, within EX28DRAFT_1385 at 1396.923 kb on - strand, within EX28DRAFT_1385 at 1396.989 kb on - strand at 1397.000 kb on + strand at 1397.001 kb on - strand at 1397.009 kb on + strand at 1397.032 kb on - strand at 1397.048 kb on - strand at 1397.048 kb on - strand at 1397.283 kb on - strand, within EX28DRAFT_1386 at 1397.410 kb on + strand, within EX28DRAFT_1386 at 1397.468 kb on + strand, within EX28DRAFT_1386 at 1397.960 kb on + strand, within EX28DRAFT_1386 at 1397.960 kb on + strand, within EX28DRAFT_1386 at 1397.961 kb on - strand, within EX28DRAFT_1386
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,395,450 - EX28DRAFT_1383 0.58 +0.2 1,395,556 + EX28DRAFT_1383 0.81 -0.0 1,395,644 + +0.4 1,395,839 - EX28DRAFT_1384 0.28 +0.4 1,395,947 - EX28DRAFT_1384 0.48 +0.7 1,396,115 + EX28DRAFT_1384 0.79 +1.2 1,396,117 + EX28DRAFT_1384 0.80 +0.4 1,396,188 - -0.6 1,396,210 - -1.4 1,396,217 - +1.8 1,396,239 - -0.4 1,396,243 + -0.0 1,396,244 - +0.2 1,396,246 + +0.9 1,396,247 - -1.0 1,396,247 - -0.1 1,396,247 - +0.2 1,396,316 + +0.1 1,396,466 - EX28DRAFT_1385 0.24 -0.8 1,396,661 - EX28DRAFT_1385 0.52 -0.4 1,396,923 - EX28DRAFT_1385 0.88 -0.7 1,396,989 - -0.7 1,397,000 + +0.0 1,397,001 - +0.2 1,397,009 + -0.1 1,397,032 - +0.6 1,397,048 - -1.8 1,397,048 - +0.3 1,397,283 - EX28DRAFT_1386 0.25 +0.3 1,397,410 + EX28DRAFT_1386 0.36 +0.7 1,397,468 + EX28DRAFT_1386 0.41 +0.5 1,397,960 + EX28DRAFT_1386 0.85 -0.1 1,397,960 + EX28DRAFT_1386 0.85 +0.1 1,397,961 - EX28DRAFT_1386 0.86 +0.4
Or see this region's nucleotide sequence