Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1216 and EX28DRAFT_1217 are separated by 156 nucleotides EX28DRAFT_1217 and EX28DRAFT_1218 are separated by 61 nucleotides EX28DRAFT_1218 and EX28DRAFT_1219 are separated by 40 nucleotides EX28DRAFT_1219 and EX28DRAFT_1220 are separated by 163 nucleotides
EX28DRAFT_1216: EX28DRAFT_1216 - Methionine synthase II (cobalamin-independent), at 1,234,941 to 1,236,044
_1216
EX28DRAFT_1217: EX28DRAFT_1217 - Rhodanese-related sulfurtransferase, at 1,236,201 to 1,236,614
_1217
EX28DRAFT_1218: EX28DRAFT_1218 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 1,236,676 to 1,237,695
_1218
EX28DRAFT_1219: EX28DRAFT_1219 - Dihydrofolate reductase, at 1,237,736 to 1,238,272
_1219
EX28DRAFT_1220: EX28DRAFT_1220 - hypothetical protein, at 1,238,436 to 1,238,798
_1220
Position (kb)
1236
1237
1238 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1236.123 kb on - strand at 1236.123 kb on - strand at 1236.144 kb on + strand at 1236.352 kb on + strand, within EX28DRAFT_1217 at 1236.436 kb on - strand, within EX28DRAFT_1217 at 1236.478 kb on - strand, within EX28DRAFT_1217 at 1236.602 kb on + strand at 1236.607 kb on + strand at 1236.649 kb on + strand at 1236.649 kb on + strand at 1236.649 kb on + strand at 1236.650 kb on - strand at 1236.650 kb on - strand at 1236.771 kb on + strand at 1236.918 kb on - strand, within EX28DRAFT_1218 at 1237.128 kb on - strand, within EX28DRAFT_1218 at 1237.207 kb on - strand, within EX28DRAFT_1218 at 1237.713 kb on + strand at 1237.713 kb on + strand at 1237.779 kb on - strand at 1237.848 kb on - strand, within EX28DRAFT_1219 at 1237.908 kb on + strand, within EX28DRAFT_1219 at 1237.931 kb on + strand, within EX28DRAFT_1219 at 1238.061 kb on + strand, within EX28DRAFT_1219 at 1238.061 kb on + strand, within EX28DRAFT_1219 at 1238.061 kb on + strand, within EX28DRAFT_1219 at 1238.061 kb on + strand, within EX28DRAFT_1219 at 1238.062 kb on - strand, within EX28DRAFT_1219 at 1238.062 kb on - strand, within EX28DRAFT_1219 at 1238.062 kb on - strand, within EX28DRAFT_1219 at 1238.152 kb on - strand, within EX28DRAFT_1219 at 1238.152 kb on - strand, within EX28DRAFT_1219 at 1238.414 kb on + strand at 1238.415 kb on - strand at 1238.415 kb on - strand at 1238.425 kb on - strand at 1238.465 kb on - strand at 1238.472 kb on - strand at 1238.543 kb on - strand, within EX28DRAFT_1220 at 1238.563 kb on + strand, within EX28DRAFT_1220 at 1238.564 kb on - strand, within EX28DRAFT_1220 at 1238.674 kb on - strand, within EX28DRAFT_1220
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,236,123 - -2.1 1,236,123 - +0.1 1,236,144 + -1.5 1,236,352 + EX28DRAFT_1217 0.36 +0.7 1,236,436 - EX28DRAFT_1217 0.57 +1.3 1,236,478 - EX28DRAFT_1217 0.67 +0.5 1,236,602 + +0.9 1,236,607 + +0.5 1,236,649 + -2.2 1,236,649 + +0.1 1,236,649 + +1.5 1,236,650 - -0.5 1,236,650 - +0.6 1,236,771 + +0.4 1,236,918 - EX28DRAFT_1218 0.24 +0.9 1,237,128 - EX28DRAFT_1218 0.44 +2.3 1,237,207 - EX28DRAFT_1218 0.52 -0.5 1,237,713 + +0.7 1,237,713 + -1.5 1,237,779 - -0.2 1,237,848 - EX28DRAFT_1219 0.21 -0.1 1,237,908 + EX28DRAFT_1219 0.32 +1.5 1,237,931 + EX28DRAFT_1219 0.36 -0.5 1,238,061 + EX28DRAFT_1219 0.61 +0.9 1,238,061 + EX28DRAFT_1219 0.61 -0.2 1,238,061 + EX28DRAFT_1219 0.61 -1.1 1,238,061 + EX28DRAFT_1219 0.61 +0.1 1,238,062 - EX28DRAFT_1219 0.61 -0.6 1,238,062 - EX28DRAFT_1219 0.61 -0.0 1,238,062 - EX28DRAFT_1219 0.61 -0.9 1,238,152 - EX28DRAFT_1219 0.77 +0.4 1,238,152 - EX28DRAFT_1219 0.77 +0.1 1,238,414 + -0.5 1,238,415 - -0.5 1,238,415 - +1.0 1,238,425 - +0.6 1,238,465 - -0.8 1,238,472 - +1.7 1,238,543 - EX28DRAFT_1220 0.29 +0.1 1,238,563 + EX28DRAFT_1220 0.35 +0.6 1,238,564 - EX28DRAFT_1220 0.35 +0.5 1,238,674 - EX28DRAFT_1220 0.66 +0.1
Or see this region's nucleotide sequence