Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1135 and EX28DRAFT_1136 are separated by 7 nucleotides EX28DRAFT_1136 and EX28DRAFT_1137 are separated by 93 nucleotides EX28DRAFT_1137 and EX28DRAFT_1138 overlap by 11 nucleotides
EX28DRAFT_1135: EX28DRAFT_1135 - Uncharacterized protein conserved in bacteria, at 1,149,343 to 1,150,635
_1135
EX28DRAFT_1136: EX28DRAFT_1136 - Uncharacterized conserved protein (DUF2132), at 1,150,643 to 1,150,930
_1136
EX28DRAFT_1137: EX28DRAFT_1137 - Beta-galactosidase/beta-glucuronidase, at 1,151,024 to 1,153,195
_1137
EX28DRAFT_1138: EX28DRAFT_1138 - Signal transduction histidine kinase, at 1,153,185 to 1,155,863
_1138
Position (kb)
1151
1152
1153
1154 Strain fitness (log2 ratio)
-1
0
1
2 at 1150.097 kb on + strand, within EX28DRAFT_1135 at 1150.097 kb on + strand, within EX28DRAFT_1135 at 1150.098 kb on - strand, within EX28DRAFT_1135 at 1150.230 kb on + strand, within EX28DRAFT_1135 at 1150.231 kb on - strand, within EX28DRAFT_1135 at 1150.353 kb on + strand, within EX28DRAFT_1135 at 1150.354 kb on - strand, within EX28DRAFT_1135 at 1150.585 kb on + strand at 1150.586 kb on - strand at 1150.621 kb on + strand at 1150.621 kb on + strand at 1150.732 kb on - strand, within EX28DRAFT_1136 at 1150.820 kb on - strand, within EX28DRAFT_1136 at 1150.854 kb on + strand, within EX28DRAFT_1136 at 1150.855 kb on - strand, within EX28DRAFT_1136 at 1150.855 kb on - strand, within EX28DRAFT_1136 at 1150.919 kb on - strand at 1150.930 kb on + strand at 1150.930 kb on + strand at 1150.930 kb on + strand at 1150.931 kb on - strand at 1151.089 kb on + strand at 1151.362 kb on - strand, within EX28DRAFT_1137 at 1151.478 kb on + strand, within EX28DRAFT_1137 at 1151.514 kb on + strand, within EX28DRAFT_1137 at 1151.528 kb on - strand, within EX28DRAFT_1137 at 1151.575 kb on + strand, within EX28DRAFT_1137 at 1151.575 kb on + strand, within EX28DRAFT_1137 at 1151.599 kb on + strand, within EX28DRAFT_1137 at 1151.605 kb on - strand, within EX28DRAFT_1137 at 1151.759 kb on + strand, within EX28DRAFT_1137 at 1151.760 kb on - strand, within EX28DRAFT_1137 at 1151.788 kb on - strand, within EX28DRAFT_1137 at 1151.830 kb on + strand, within EX28DRAFT_1137 at 1152.223 kb on - strand, within EX28DRAFT_1137 at 1152.365 kb on - strand, within EX28DRAFT_1137 at 1152.366 kb on + strand, within EX28DRAFT_1137 at 1152.367 kb on - strand, within EX28DRAFT_1137 at 1152.367 kb on - strand, within EX28DRAFT_1137 at 1152.868 kb on - strand, within EX28DRAFT_1137 at 1152.918 kb on + strand, within EX28DRAFT_1137 at 1152.988 kb on + strand at 1152.989 kb on - strand at 1152.989 kb on - strand at 1152.989 kb on - strand at 1152.991 kb on - strand at 1153.186 kb on + strand at 1153.253 kb on - strand at 1153.253 kb on - strand at 1153.295 kb on + strand at 1153.295 kb on + strand at 1153.352 kb on + strand at 1153.353 kb on - strand at 1153.479 kb on + strand, within EX28DRAFT_1138 at 1153.510 kb on - strand, within EX28DRAFT_1138 at 1153.519 kb on - strand, within EX28DRAFT_1138 at 1153.613 kb on - strand, within EX28DRAFT_1138 at 1153.632 kb on - strand, within EX28DRAFT_1138 at 1153.789 kb on - strand, within EX28DRAFT_1138 at 1154.027 kb on + strand, within EX28DRAFT_1138 at 1154.028 kb on - strand, within EX28DRAFT_1138
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,150,097 + EX28DRAFT_1135 0.58 -0.2 1,150,097 + EX28DRAFT_1135 0.58 -0.0 1,150,098 - EX28DRAFT_1135 0.58 +0.1 1,150,230 + EX28DRAFT_1135 0.69 -0.7 1,150,231 - EX28DRAFT_1135 0.69 +0.0 1,150,353 + EX28DRAFT_1135 0.78 -0.4 1,150,354 - EX28DRAFT_1135 0.78 -0.2 1,150,585 + -0.0 1,150,586 - -0.3 1,150,621 + -0.6 1,150,621 + +0.1 1,150,732 - EX28DRAFT_1136 0.31 +0.4 1,150,820 - EX28DRAFT_1136 0.61 +0.3 1,150,854 + EX28DRAFT_1136 0.73 -0.4 1,150,855 - EX28DRAFT_1136 0.74 -0.3 1,150,855 - EX28DRAFT_1136 0.74 -0.5 1,150,919 - -0.3 1,150,930 + +0.3 1,150,930 + -1.6 1,150,930 + +0.1 1,150,931 - +0.5 1,151,089 + +0.6 1,151,362 - EX28DRAFT_1137 0.16 -0.1 1,151,478 + EX28DRAFT_1137 0.21 +1.6 1,151,514 + EX28DRAFT_1137 0.23 -0.3 1,151,528 - EX28DRAFT_1137 0.23 +0.0 1,151,575 + EX28DRAFT_1137 0.25 -0.3 1,151,575 + EX28DRAFT_1137 0.25 -1.0 1,151,599 + EX28DRAFT_1137 0.26 -1.0 1,151,605 - EX28DRAFT_1137 0.27 -1.4 1,151,759 + EX28DRAFT_1137 0.34 -0.4 1,151,760 - EX28DRAFT_1137 0.34 +0.6 1,151,788 - EX28DRAFT_1137 0.35 -1.4 1,151,830 + EX28DRAFT_1137 0.37 +0.6 1,152,223 - EX28DRAFT_1137 0.55 +0.1 1,152,365 - EX28DRAFT_1137 0.62 -0.1 1,152,366 + EX28DRAFT_1137 0.62 +1.8 1,152,367 - EX28DRAFT_1137 0.62 -0.3 1,152,367 - EX28DRAFT_1137 0.62 -0.1 1,152,868 - EX28DRAFT_1137 0.85 +0.3 1,152,918 + EX28DRAFT_1137 0.87 +0.3 1,152,988 + +0.8 1,152,989 - +1.1 1,152,989 - -1.0 1,152,989 - +0.6 1,152,991 - -0.2 1,153,186 + -0.2 1,153,253 - -0.0 1,153,253 - +0.2 1,153,295 + -0.1 1,153,295 + +1.0 1,153,352 + -0.0 1,153,353 - -1.3 1,153,479 + EX28DRAFT_1138 0.11 +0.1 1,153,510 - EX28DRAFT_1138 0.12 -0.2 1,153,519 - EX28DRAFT_1138 0.12 +0.4 1,153,613 - EX28DRAFT_1138 0.16 -0.7 1,153,632 - EX28DRAFT_1138 0.17 -0.6 1,153,789 - EX28DRAFT_1138 0.23 -0.4 1,154,027 + EX28DRAFT_1138 0.31 -0.1 1,154,028 - EX28DRAFT_1138 0.31 +0.5
Or see this region's nucleotide sequence