Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1010 and EX28DRAFT_1011 are separated by 787 nucleotides EX28DRAFT_1011 and EX28DRAFT_1012 are separated by 496 nucleotides EX28DRAFT_1012 and EX28DRAFT_1013 are separated by 143 nucleotides
EX28DRAFT_1010: EX28DRAFT_1010 - Cold shock proteins, at 1,015,739 to 1,015,957
_1010
EX28DRAFT_1011: EX28DRAFT_1011 - FOG: EAL domain, at 1,016,745 to 1,018,268
_1011
EX28DRAFT_1012: EX28DRAFT_1012 - Predicted membrane protein, at 1,018,765 to 1,019,109
_1012
EX28DRAFT_1013: EX28DRAFT_1013 - hypothetical protein, at 1,019,253 to 1,019,702
_1013
Position (kb)
1016
1017
1018
1019 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1015.962 kb on - strand at 1016.280 kb on - strand at 1016.280 kb on - strand at 1016.281 kb on + strand at 1016.282 kb on - strand at 1016.295 kb on + strand at 1016.295 kb on + strand at 1016.295 kb on + strand at 1016.296 kb on - strand at 1016.300 kb on - strand at 1016.300 kb on - strand at 1016.364 kb on + strand at 1016.430 kb on + strand at 1016.431 kb on - strand at 1016.549 kb on + strand at 1016.560 kb on + strand at 1016.561 kb on - strand at 1016.564 kb on - strand at 1016.571 kb on + strand at 1016.652 kb on - strand at 1016.655 kb on + strand at 1016.729 kb on - strand at 1016.742 kb on + strand at 1016.759 kb on + strand at 1016.862 kb on + strand at 1016.862 kb on + strand at 1016.862 kb on + strand at 1016.863 kb on - strand at 1016.885 kb on + strand at 1017.148 kb on + strand, within EX28DRAFT_1011 at 1017.230 kb on - strand, within EX28DRAFT_1011 at 1017.243 kb on + strand, within EX28DRAFT_1011 at 1017.244 kb on - strand, within EX28DRAFT_1011 at 1017.256 kb on + strand, within EX28DRAFT_1011 at 1017.319 kb on + strand, within EX28DRAFT_1011 at 1017.436 kb on + strand, within EX28DRAFT_1011 at 1017.476 kb on + strand, within EX28DRAFT_1011 at 1017.671 kb on + strand, within EX28DRAFT_1011 at 1017.671 kb on + strand, within EX28DRAFT_1011 at 1017.691 kb on + strand, within EX28DRAFT_1011 at 1017.692 kb on - strand, within EX28DRAFT_1011 at 1017.709 kb on + strand, within EX28DRAFT_1011 at 1017.710 kb on - strand, within EX28DRAFT_1011 at 1017.722 kb on + strand, within EX28DRAFT_1011 at 1017.755 kb on - strand, within EX28DRAFT_1011 at 1017.761 kb on + strand, within EX28DRAFT_1011 at 1017.779 kb on + strand, within EX28DRAFT_1011 at 1017.923 kb on + strand, within EX28DRAFT_1011 at 1017.923 kb on + strand, within EX28DRAFT_1011 at 1017.923 kb on + strand, within EX28DRAFT_1011 at 1017.999 kb on - strand, within EX28DRAFT_1011 at 1018.010 kb on + strand, within EX28DRAFT_1011 at 1018.106 kb on + strand, within EX28DRAFT_1011 at 1018.150 kb on - strand at 1018.194 kb on + strand at 1018.512 kb on - strand at 1018.628 kb on + strand at 1018.628 kb on + strand at 1018.629 kb on - strand at 1018.680 kb on + strand at 1018.721 kb on - strand at 1018.721 kb on - strand at 1018.764 kb on + strand at 1018.882 kb on + strand, within EX28DRAFT_1012 at 1018.882 kb on + strand, within EX28DRAFT_1012 at 1018.882 kb on + strand, within EX28DRAFT_1012 at 1018.882 kb on + strand, within EX28DRAFT_1012 at 1018.883 kb on - strand, within EX28DRAFT_1012 at 1018.893 kb on + strand, within EX28DRAFT_1012 at 1019.005 kb on - strand, within EX28DRAFT_1012 at 1019.033 kb on - strand, within EX28DRAFT_1012 at 1019.080 kb on + strand at 1019.140 kb on + strand at 1019.140 kb on + strand at 1019.183 kb on + strand at 1019.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,015,962 - +0.1 1,016,280 - -0.8 1,016,280 - +0.6 1,016,281 + -0.1 1,016,282 - -0.4 1,016,295 + +0.5 1,016,295 + +0.2 1,016,295 + -0.6 1,016,296 - +0.8 1,016,300 - +0.4 1,016,300 - -0.4 1,016,364 + +0.9 1,016,430 + -1.6 1,016,431 - -3.4 1,016,549 + -1.0 1,016,560 + -1.4 1,016,561 - +1.6 1,016,564 - -1.3 1,016,571 + +0.2 1,016,652 - -0.6 1,016,655 + +0.1 1,016,729 - +0.2 1,016,742 + +0.1 1,016,759 + +0.5 1,016,862 + -1.9 1,016,862 + -0.5 1,016,862 + +0.2 1,016,863 - +0.2 1,016,885 + -0.2 1,017,148 + EX28DRAFT_1011 0.26 +0.8 1,017,230 - EX28DRAFT_1011 0.32 -0.4 1,017,243 + EX28DRAFT_1011 0.33 +0.8 1,017,244 - EX28DRAFT_1011 0.33 -2.4 1,017,256 + EX28DRAFT_1011 0.34 -1.1 1,017,319 + EX28DRAFT_1011 0.38 -0.7 1,017,436 + EX28DRAFT_1011 0.45 -0.4 1,017,476 + EX28DRAFT_1011 0.48 -0.0 1,017,671 + EX28DRAFT_1011 0.61 +1.6 1,017,671 + EX28DRAFT_1011 0.61 +0.4 1,017,691 + EX28DRAFT_1011 0.62 +0.2 1,017,692 - EX28DRAFT_1011 0.62 +0.1 1,017,709 + EX28DRAFT_1011 0.63 -0.3 1,017,710 - EX28DRAFT_1011 0.63 -0.4 1,017,722 + EX28DRAFT_1011 0.64 +0.0 1,017,755 - EX28DRAFT_1011 0.66 -0.8 1,017,761 + EX28DRAFT_1011 0.67 -0.8 1,017,779 + EX28DRAFT_1011 0.68 +0.0 1,017,923 + EX28DRAFT_1011 0.77 -0.1 1,017,923 + EX28DRAFT_1011 0.77 -2.0 1,017,923 + EX28DRAFT_1011 0.77 +0.2 1,017,999 - EX28DRAFT_1011 0.82 -0.2 1,018,010 + EX28DRAFT_1011 0.83 +0.0 1,018,106 + EX28DRAFT_1011 0.89 +0.2 1,018,150 - +1.1 1,018,194 + -1.2 1,018,512 - -0.7 1,018,628 + -0.2 1,018,628 + -0.7 1,018,629 - -0.2 1,018,680 + +0.4 1,018,721 - -0.4 1,018,721 - +0.3 1,018,764 + +0.3 1,018,882 + EX28DRAFT_1012 0.34 -0.0 1,018,882 + EX28DRAFT_1012 0.34 -0.1 1,018,882 + EX28DRAFT_1012 0.34 +0.4 1,018,882 + EX28DRAFT_1012 0.34 +0.6 1,018,883 - EX28DRAFT_1012 0.34 -0.7 1,018,893 + EX28DRAFT_1012 0.37 +0.2 1,019,005 - EX28DRAFT_1012 0.70 -0.4 1,019,033 - EX28DRAFT_1012 0.78 +0.4 1,019,080 + +0.1 1,019,140 + +0.6 1,019,140 + -0.8 1,019,183 + -0.4 1,019,224 - -0.1
Or see this region's nucleotide sequence