Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0611 and EX28DRAFT_0612 overlap by 4 nucleotides EX28DRAFT_0612 and EX28DRAFT_0613 are separated by 21 nucleotides EX28DRAFT_0613 and EX28DRAFT_0614 overlap by 1 nucleotides EX28DRAFT_0614 and EX28DRAFT_0615 overlap by 8 nucleotides
EX28DRAFT_0611: EX28DRAFT_0611 - Flagellar hook-length control protein, at 608,998 to 610,233
_0611
EX28DRAFT_0612: EX28DRAFT_0612 - flagellar export protein FliJ, at 610,230 to 610,673
_0612
EX28DRAFT_0613: EX28DRAFT_0613 - flagellar protein export ATPase FliI, at 610,695 to 612,065
_0613
EX28DRAFT_0614: EX28DRAFT_0614 - Flagellar biosynthesis/type III secretory pathway protein, at 612,065 to 612,772
_0614
EX28DRAFT_0615: EX28DRAFT_0615 - flagellar motor switch protein FliG, at 612,765 to 613,763
_0615
Position (kb)
610
611
612
613 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 609.774 kb on + strand, within EX28DRAFT_0611 at 609.849 kb on + strand, within EX28DRAFT_0611 at 609.900 kb on + strand, within EX28DRAFT_0611 at 609.931 kb on - strand, within EX28DRAFT_0611 at 610.332 kb on + strand, within EX28DRAFT_0612 at 610.332 kb on + strand, within EX28DRAFT_0612 at 610.469 kb on - strand, within EX28DRAFT_0612 at 610.469 kb on - strand, within EX28DRAFT_0612 at 610.473 kb on - strand, within EX28DRAFT_0612 at 610.473 kb on - strand, within EX28DRAFT_0612 at 610.599 kb on + strand, within EX28DRAFT_0612 at 610.696 kb on + strand at 610.696 kb on + strand at 610.697 kb on - strand at 610.926 kb on + strand, within EX28DRAFT_0613 at 611.015 kb on + strand, within EX28DRAFT_0613 at 611.015 kb on + strand, within EX28DRAFT_0613 at 611.015 kb on + strand, within EX28DRAFT_0613 at 611.016 kb on - strand, within EX28DRAFT_0613 at 611.016 kb on - strand, within EX28DRAFT_0613 at 611.082 kb on + strand, within EX28DRAFT_0613 at 611.082 kb on + strand, within EX28DRAFT_0613 at 611.087 kb on + strand, within EX28DRAFT_0613 at 611.089 kb on + strand, within EX28DRAFT_0613 at 611.090 kb on - strand, within EX28DRAFT_0613 at 611.409 kb on - strand, within EX28DRAFT_0613 at 611.419 kb on - strand, within EX28DRAFT_0613 at 611.473 kb on + strand, within EX28DRAFT_0613 at 611.504 kb on + strand, within EX28DRAFT_0613 at 611.505 kb on - strand, within EX28DRAFT_0613 at 611.534 kb on + strand, within EX28DRAFT_0613 at 611.535 kb on - strand, within EX28DRAFT_0613 at 611.771 kb on + strand, within EX28DRAFT_0613 at 612.004 kb on - strand at 612.170 kb on + strand, within EX28DRAFT_0614 at 612.171 kb on - strand, within EX28DRAFT_0614 at 612.584 kb on - strand, within EX28DRAFT_0614 at 612.585 kb on + strand, within EX28DRAFT_0614 at 612.585 kb on + strand, within EX28DRAFT_0614 at 612.586 kb on - strand, within EX28DRAFT_0614 at 612.766 kb on + strand at 612.767 kb on - strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.772 kb on + strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand at 612.773 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 609,774 + EX28DRAFT_0611 0.63 +0.5 609,849 + EX28DRAFT_0611 0.69 +0.2 609,900 + EX28DRAFT_0611 0.73 +1.1 609,931 - EX28DRAFT_0611 0.75 -0.3 610,332 + EX28DRAFT_0612 0.23 +2.1 610,332 + EX28DRAFT_0612 0.23 -0.1 610,469 - EX28DRAFT_0612 0.54 +0.7 610,469 - EX28DRAFT_0612 0.54 +1.3 610,473 - EX28DRAFT_0612 0.55 +0.9 610,473 - EX28DRAFT_0612 0.55 +0.3 610,599 + EX28DRAFT_0612 0.83 +0.9 610,696 + -0.6 610,696 + +1.4 610,697 - -2.8 610,926 + EX28DRAFT_0613 0.17 -0.0 611,015 + EX28DRAFT_0613 0.23 +0.1 611,015 + EX28DRAFT_0613 0.23 +0.4 611,015 + EX28DRAFT_0613 0.23 +0.4 611,016 - EX28DRAFT_0613 0.23 -0.5 611,016 - EX28DRAFT_0613 0.23 -0.4 611,082 + EX28DRAFT_0613 0.28 -0.2 611,082 + EX28DRAFT_0613 0.28 +0.8 611,087 + EX28DRAFT_0613 0.29 +0.7 611,089 + EX28DRAFT_0613 0.29 -1.6 611,090 - EX28DRAFT_0613 0.29 +1.2 611,409 - EX28DRAFT_0613 0.52 +0.2 611,419 - EX28DRAFT_0613 0.53 +0.1 611,473 + EX28DRAFT_0613 0.57 -0.1 611,504 + EX28DRAFT_0613 0.59 +0.2 611,505 - EX28DRAFT_0613 0.59 +1.0 611,534 + EX28DRAFT_0613 0.61 +1.0 611,535 - EX28DRAFT_0613 0.61 -0.1 611,771 + EX28DRAFT_0613 0.78 +0.5 612,004 - +1.1 612,170 + EX28DRAFT_0614 0.15 +0.4 612,171 - EX28DRAFT_0614 0.15 +0.2 612,584 - EX28DRAFT_0614 0.73 +1.1 612,585 + EX28DRAFT_0614 0.73 -0.2 612,585 + EX28DRAFT_0614 0.73 -0.7 612,586 - EX28DRAFT_0614 0.74 +0.3 612,766 + -0.2 612,767 - +1.3 612,772 + -0.1 612,772 + +0.1 612,772 + +0.9 612,772 + +0.3 612,772 + -0.7 612,772 + +0.4 612,772 + -0.4 612,772 + +0.4 612,772 + -1.3 612,772 + +0.5 612,772 + -0.2 612,772 + +0.5 612,772 + -0.1 612,772 + +0.3 612,772 + +0.1 612,772 + -0.5 612,772 + +1.3 612,772 + +1.0 612,772 + -0.5 612,773 - -0.8 612,773 - +0.6 612,773 - -0.1 612,773 - -0.9 612,773 - -0.0 612,773 - -0.7 612,773 - +0.9 612,773 - +1.4 612,773 - +0.1 612,773 - -1.0 612,773 - +0.3 612,773 - +0.4 612,773 - -0.9 612,773 - -2.4 612,773 - +0.4 612,773 - +0.6 612,773 - +0.3 612,773 - +0.9
Or see this region's nucleotide sequence