Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0532 and EX28DRAFT_0533 are separated by 40 nucleotides EX28DRAFT_0533 and EX28DRAFT_0534 overlap by 7 nucleotides EX28DRAFT_0534 and EX28DRAFT_0535 overlap by 19 nucleotides EX28DRAFT_0535 and EX28DRAFT_0536 overlap by 1 nucleotides
EX28DRAFT_0532: EX28DRAFT_0532 - Chain length determinant protein, at 527,068 to 528,048
_0532
EX28DRAFT_0533: EX28DRAFT_0533 - phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 528,089 to 528,700
_0533
EX28DRAFT_0534: EX28DRAFT_0534 - imidazoleglycerol phosphate synthase, cyclase subunit, at 528,694 to 529,470
_0534
EX28DRAFT_0535: EX28DRAFT_0535 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC 5.3.1.16), at 529,452 to 530,189
_0535
EX28DRAFT_0536: EX28DRAFT_0536 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 530,189 to 530,779
_0536
Position (kb) 528
529
530 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.830 kb on + strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.886 kb on + strand, within EX28DRAFT_0532 at 527.887 kb on - strand, within EX28DRAFT_0532 at 527.896 kb on + strand, within EX28DRAFT_0532 at 527.999 kb on + strand at 528.023 kb on + strand at 528.031 kb on + strand at 528.075 kb on + strand at 528.168 kb on + strand, within EX28DRAFT_0533 at 528.168 kb on + strand, within EX28DRAFT_0533 at 528.212 kb on + strand, within EX28DRAFT_0533 at 528.213 kb on - strand, within EX28DRAFT_0533 at 528.213 kb on - strand, within EX28DRAFT_0533 at 528.265 kb on + strand, within EX28DRAFT_0533 at 528.266 kb on - strand, within EX28DRAFT_0533 at 528.301 kb on - strand, within EX28DRAFT_0533 at 528.301 kb on - strand, within EX28DRAFT_0533 at 528.592 kb on + strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.595 kb on - strand, within EX28DRAFT_0533 at 528.731 kb on + strand at 528.750 kb on + strand at 528.751 kb on - strand at 528.751 kb on - strand at 529.053 kb on - strand, within EX28DRAFT_0534 at 529.074 kb on - strand, within EX28DRAFT_0534 at 529.098 kb on + strand, within EX28DRAFT_0534 at 529.099 kb on - strand, within EX28DRAFT_0534 at 529.177 kb on - strand, within EX28DRAFT_0534 at 529.322 kb on + strand, within EX28DRAFT_0534 at 529.323 kb on - strand, within EX28DRAFT_0534 at 529.435 kb on + strand at 529.450 kb on + strand at 529.450 kb on + strand at 529.450 kb on + strand at 529.451 kb on - strand at 529.451 kb on - strand at 529.490 kb on + strand at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.567 kb on - strand, within EX28DRAFT_0535 at 530.055 kb on + strand, within EX28DRAFT_0535 at 530.055 kb on + strand, within EX28DRAFT_0535 at 530.157 kb on - strand at 530.184 kb on - strand at 530.305 kb on - strand, within EX28DRAFT_0536 at 530.346 kb on + strand, within EX28DRAFT_0536
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 527,735 + EX28DRAFT_0532 0.68 -2.3 527,735 + EX28DRAFT_0532 0.68 -0.9 527,830 + EX28DRAFT_0532 0.78 +0.5 527,874 - EX28DRAFT_0532 0.82 -0.2 527,874 - EX28DRAFT_0532 0.82 -1.0 527,886 + EX28DRAFT_0532 0.83 -0.1 527,887 - EX28DRAFT_0532 0.83 +1.4 527,896 + EX28DRAFT_0532 0.84 +0.0 527,999 + +0.3 528,023 + +0.1 528,031 + -0.5 528,075 + +1.0 528,168 + EX28DRAFT_0533 0.13 -0.3 528,168 + EX28DRAFT_0533 0.13 -3.4 528,212 + EX28DRAFT_0533 0.20 -2.8 528,213 - EX28DRAFT_0533 0.20 -1.8 528,213 - EX28DRAFT_0533 0.20 -3.8 528,265 + EX28DRAFT_0533 0.29 -1.5 528,266 - EX28DRAFT_0533 0.29 -3.1 528,301 - EX28DRAFT_0533 0.35 -1.7 528,301 - EX28DRAFT_0533 0.35 -4.2 528,592 + EX28DRAFT_0533 0.82 -4.1 528,593 - EX28DRAFT_0533 0.82 -3.8 528,593 - EX28DRAFT_0533 0.82 -3.5 528,593 - EX28DRAFT_0533 0.82 -2.9 528,593 - EX28DRAFT_0533 0.82 -5.1 528,593 - EX28DRAFT_0533 0.82 -4.3 528,595 - EX28DRAFT_0533 0.83 -2.8 528,731 + -3.2 528,750 + -5.6 528,751 - -3.4 528,751 - -4.0 529,053 - EX28DRAFT_0534 0.46 -5.7 529,074 - EX28DRAFT_0534 0.49 -3.7 529,098 + EX28DRAFT_0534 0.52 -4.4 529,099 - EX28DRAFT_0534 0.52 -3.1 529,177 - EX28DRAFT_0534 0.62 -0.1 529,322 + EX28DRAFT_0534 0.81 -4.3 529,323 - EX28DRAFT_0534 0.81 -3.4 529,435 + -3.5 529,450 + -3.2 529,450 + -2.5 529,450 + -1.1 529,451 - -3.7 529,451 - -4.3 529,490 + -3.4 529,566 + EX28DRAFT_0535 0.15 -4.0 529,566 + EX28DRAFT_0535 0.15 -5.1 529,566 + EX28DRAFT_0535 0.15 -2.4 529,567 - EX28DRAFT_0535 0.16 -6.7 530,055 + EX28DRAFT_0535 0.82 -2.5 530,055 + EX28DRAFT_0535 0.82 -4.2 530,157 - -2.9 530,184 - -1.6 530,305 - EX28DRAFT_0536 0.20 -3.9 530,346 + EX28DRAFT_0536 0.27 -3.4
Or see this region's nucleotide sequence