Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0409 and EX28DRAFT_0410 are separated by 49 nucleotides EX28DRAFT_0410 and EX28DRAFT_0411 are separated by 124 nucleotides EX28DRAFT_0411 and EX28DRAFT_0412 are separated by 126 nucleotides
EX28DRAFT_0409: EX28DRAFT_0409 - ABC-type glucose/galactose transport system, permease component, at 386,991 to 388,001
_0409
EX28DRAFT_0410: EX28DRAFT_0410 - Glutamine synthetase, at 388,051 to 389,439
_0410
EX28DRAFT_0411: EX28DRAFT_0411 - Uncharacterized membrane protein, at 389,564 to 390,280
_0411
EX28DRAFT_0412: EX28DRAFT_0412 - cytidine deaminase (EC 3.5.4.5), at 390,407 to 391,291
_0412
Position (kb) 388
389
390 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 387.059 kb on - strand at 387.061 kb on + strand at 387.093 kb on - strand, within EX28DRAFT_0409 at 387.100 kb on + strand, within EX28DRAFT_0409 at 387.146 kb on + strand, within EX28DRAFT_0409 at 387.147 kb on - strand, within EX28DRAFT_0409 at 387.362 kb on + strand, within EX28DRAFT_0409 at 387.365 kb on + strand, within EX28DRAFT_0409 at 387.375 kb on + strand, within EX28DRAFT_0409 at 387.411 kb on - strand, within EX28DRAFT_0409 at 387.444 kb on + strand, within EX28DRAFT_0409 at 387.445 kb on - strand, within EX28DRAFT_0409 at 387.543 kb on + strand, within EX28DRAFT_0409 at 387.543 kb on + strand, within EX28DRAFT_0409 at 387.543 kb on + strand, within EX28DRAFT_0409 at 387.544 kb on - strand, within EX28DRAFT_0409 at 387.544 kb on - strand, within EX28DRAFT_0409 at 387.544 kb on - strand, within EX28DRAFT_0409 at 387.545 kb on + strand, within EX28DRAFT_0409 at 387.545 kb on + strand, within EX28DRAFT_0409 at 387.555 kb on + strand, within EX28DRAFT_0409 at 387.714 kb on + strand, within EX28DRAFT_0409 at 387.780 kb on + strand, within EX28DRAFT_0409 at 387.781 kb on - strand, within EX28DRAFT_0409 at 387.930 kb on + strand at 387.936 kb on - strand at 387.985 kb on - strand at 387.999 kb on + strand at 387.999 kb on + strand at 388.000 kb on - strand at 388.045 kb on + strand at 388.046 kb on - strand at 388.052 kb on + strand at 388.164 kb on - strand at 388.399 kb on - strand, within EX28DRAFT_0410 at 388.400 kb on + strand, within EX28DRAFT_0410 at 388.414 kb on - strand, within EX28DRAFT_0410 at 388.537 kb on - strand, within EX28DRAFT_0410 at 388.747 kb on + strand, within EX28DRAFT_0410 at 388.755 kb on - strand, within EX28DRAFT_0410 at 388.855 kb on - strand, within EX28DRAFT_0410 at 388.858 kb on - strand, within EX28DRAFT_0410 at 388.858 kb on - strand, within EX28DRAFT_0410 at 388.985 kb on - strand, within EX28DRAFT_0410 at 389.013 kb on + strand, within EX28DRAFT_0410 at 389.266 kb on + strand, within EX28DRAFT_0410 at 389.266 kb on + strand, within EX28DRAFT_0410 at 389.266 kb on + strand, within EX28DRAFT_0410 at 389.267 kb on - strand, within EX28DRAFT_0410 at 389.386 kb on + strand at 389.440 kb on + strand at 389.441 kb on - strand at 389.494 kb on - strand at 389.549 kb on - strand at 389.550 kb on + strand at 389.551 kb on - strand at 389.566 kb on - strand at 389.585 kb on - strand at 389.672 kb on + strand, within EX28DRAFT_0411 at 389.700 kb on + strand, within EX28DRAFT_0411 at 389.700 kb on + strand, within EX28DRAFT_0411 at 389.701 kb on - strand, within EX28DRAFT_0411 at 389.759 kb on - strand, within EX28DRAFT_0411 at 389.781 kb on + strand, within EX28DRAFT_0411 at 389.804 kb on - strand, within EX28DRAFT_0411 at 390.030 kb on + strand, within EX28DRAFT_0411 at 390.030 kb on + strand, within EX28DRAFT_0411 at 390.030 kb on + strand, within EX28DRAFT_0411 at 390.051 kb on + strand, within EX28DRAFT_0411 at 390.052 kb on - strand, within EX28DRAFT_0411 at 390.088 kb on - strand, within EX28DRAFT_0411 at 390.114 kb on + strand, within EX28DRAFT_0411 at 390.115 kb on - strand, within EX28DRAFT_0411 at 390.180 kb on + strand, within EX28DRAFT_0411 at 390.181 kb on - strand, within EX28DRAFT_0411 at 390.237 kb on - strand at 390.237 kb on - strand at 390.318 kb on + strand at 390.419 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 387,059 - -1.2 387,061 + +1.0 387,093 - EX28DRAFT_0409 0.10 +0.4 387,100 + EX28DRAFT_0409 0.11 +0.3 387,146 + EX28DRAFT_0409 0.15 +0.2 387,147 - EX28DRAFT_0409 0.15 -0.3 387,362 + EX28DRAFT_0409 0.37 +0.5 387,365 + EX28DRAFT_0409 0.37 -0.5 387,375 + EX28DRAFT_0409 0.38 +0.2 387,411 - EX28DRAFT_0409 0.42 -1.1 387,444 + EX28DRAFT_0409 0.45 +0.9 387,445 - EX28DRAFT_0409 0.45 -0.4 387,543 + EX28DRAFT_0409 0.55 +2.8 387,543 + EX28DRAFT_0409 0.55 +0.7 387,543 + EX28DRAFT_0409 0.55 +0.3 387,544 - EX28DRAFT_0409 0.55 -0.2 387,544 - EX28DRAFT_0409 0.55 +1.8 387,544 - EX28DRAFT_0409 0.55 -1.3 387,545 + EX28DRAFT_0409 0.55 +0.9 387,545 + EX28DRAFT_0409 0.55 -0.1 387,555 + EX28DRAFT_0409 0.56 -0.0 387,714 + EX28DRAFT_0409 0.72 +0.6 387,780 + EX28DRAFT_0409 0.78 +0.8 387,781 - EX28DRAFT_0409 0.78 -1.5 387,930 + +0.1 387,936 - +0.1 387,985 - -0.8 387,999 + -0.4 387,999 + +0.9 388,000 - -0.2 388,045 + -0.6 388,046 - -0.2 388,052 + -0.1 388,164 - -0.5 388,399 - EX28DRAFT_0410 0.25 +0.2 388,400 + EX28DRAFT_0410 0.25 -1.4 388,414 - EX28DRAFT_0410 0.26 +1.6 388,537 - EX28DRAFT_0410 0.35 +0.4 388,747 + EX28DRAFT_0410 0.50 -0.6 388,755 - EX28DRAFT_0410 0.51 +0.4 388,855 - EX28DRAFT_0410 0.58 -0.8 388,858 - EX28DRAFT_0410 0.58 +2.3 388,858 - EX28DRAFT_0410 0.58 +0.5 388,985 - EX28DRAFT_0410 0.67 -0.1 389,013 + EX28DRAFT_0410 0.69 +0.4 389,266 + EX28DRAFT_0410 0.87 -0.3 389,266 + EX28DRAFT_0410 0.87 -1.3 389,266 + EX28DRAFT_0410 0.87 +0.2 389,267 - EX28DRAFT_0410 0.88 +0.3 389,386 + -0.7 389,440 + -1.3 389,441 - +0.4 389,494 - -0.9 389,549 - -0.2 389,550 + +0.2 389,551 - -0.9 389,566 - +0.9 389,585 - +0.3 389,672 + EX28DRAFT_0411 0.15 -0.3 389,700 + EX28DRAFT_0411 0.19 -1.2 389,700 + EX28DRAFT_0411 0.19 -1.0 389,701 - EX28DRAFT_0411 0.19 -2.1 389,759 - EX28DRAFT_0411 0.27 -3.3 389,781 + EX28DRAFT_0411 0.30 -0.2 389,804 - EX28DRAFT_0411 0.33 -2.3 390,030 + EX28DRAFT_0411 0.65 +0.6 390,030 + EX28DRAFT_0411 0.65 +0.4 390,030 + EX28DRAFT_0411 0.65 -0.4 390,051 + EX28DRAFT_0411 0.68 -0.1 390,052 - EX28DRAFT_0411 0.68 -0.5 390,088 - EX28DRAFT_0411 0.73 -1.2 390,114 + EX28DRAFT_0411 0.77 -0.0 390,115 - EX28DRAFT_0411 0.77 -1.0 390,180 + EX28DRAFT_0411 0.86 +0.3 390,181 - EX28DRAFT_0411 0.86 -0.1 390,237 - +0.3 390,237 - -0.5 390,318 + -0.5 390,419 + +2.1
Or see this region's nucleotide sequence