Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0140 and EX28DRAFT_0141 are separated by 145 nucleotides EX28DRAFT_0141 and EX28DRAFT_0142 are separated by 160 nucleotides EX28DRAFT_0142 and EX28DRAFT_0143 are separated by 715 nucleotides
EX28DRAFT_0140: EX28DRAFT_0140 - GMP synthase (glutamine-hydrolyzing), C-terminal domain or B subunit/GMP synthase (glutamine-hydrolyzing), N-terminal domain or A subunit, at 84,605 to 86,182
_0140
EX28DRAFT_0141: EX28DRAFT_0141 - hypothetical protein, at 86,328 to 86,546
_0141
EX28DRAFT_0142: EX28DRAFT_0142 - hypothetical protein, at 86,707 to 86,883
_0142
EX28DRAFT_0143: EX28DRAFT_0143 - hypothetical protein, at 87,599 to 87,674
_0143
Position (kb)
86
87 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 86.223 kb on + strand at 86.229 kb on + strand at 86.304 kb on - strand at 86.308 kb on + strand at 86.308 kb on + strand at 86.308 kb on + strand at 86.308 kb on + strand at 86.308 kb on + strand at 86.309 kb on - strand at 86.309 kb on - strand at 86.309 kb on - strand at 86.322 kb on - strand at 86.482 kb on - strand, within EX28DRAFT_0141 at 86.482 kb on - strand, within EX28DRAFT_0141 at 86.501 kb on + strand, within EX28DRAFT_0141 at 86.535 kb on - strand at 86.535 kb on - strand at 86.536 kb on + strand at 86.536 kb on + strand at 86.536 kb on + strand at 86.536 kb on + strand at 86.536 kb on + strand at 86.537 kb on - strand at 86.537 kb on - strand at 86.537 kb on - strand at 86.537 kb on - strand at 86.537 kb on - strand at 86.538 kb on + strand at 86.539 kb on - strand at 86.539 kb on - strand at 86.539 kb on - strand at 86.547 kb on - strand at 86.592 kb on + strand at 86.592 kb on + strand at 86.593 kb on - strand at 86.593 kb on - strand at 86.615 kb on - strand at 86.622 kb on - strand at 86.627 kb on + strand at 86.627 kb on + strand at 86.699 kb on + strand at 86.722 kb on - strand at 86.752 kb on - strand, within EX28DRAFT_0142 at 86.760 kb on + strand, within EX28DRAFT_0142 at 86.761 kb on - strand, within EX28DRAFT_0142 at 86.795 kb on - strand, within EX28DRAFT_0142 at 86.849 kb on + strand, within EX28DRAFT_0142 at 86.849 kb on + strand, within EX28DRAFT_0142 at 86.850 kb on - strand, within EX28DRAFT_0142 at 86.865 kb on + strand, within EX28DRAFT_0142 at 86.941 kb on + strand at 86.986 kb on - strand at 86.986 kb on - strand at 86.998 kb on + strand at 87.018 kb on - strand at 87.035 kb on - strand at 87.035 kb on - strand at 87.038 kb on + strand at 87.038 kb on + strand at 87.050 kb on - strand at 87.083 kb on - strand at 87.217 kb on - strand at 87.530 kb on + strand at 87.585 kb on - strand at 87.702 kb on - strand at 87.702 kb on - strand at 87.702 kb on - strand at 87.702 kb on - strand at 87.804 kb on + strand at 87.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 86,223 + -0.5 86,229 + +0.0 86,304 - -1.5 86,308 + +0.3 86,308 + -1.2 86,308 + +0.9 86,308 + +0.4 86,308 + -0.3 86,309 - +1.2 86,309 - +0.7 86,309 - -0.1 86,322 - -0.3 86,482 - EX28DRAFT_0141 0.70 +0.1 86,482 - EX28DRAFT_0141 0.70 -0.6 86,501 + EX28DRAFT_0141 0.79 +0.3 86,535 - -0.2 86,535 - -0.5 86,536 + -0.1 86,536 + -0.2 86,536 + -0.1 86,536 + -1.1 86,536 + -0.1 86,537 - -0.2 86,537 - -0.5 86,537 - +0.0 86,537 - -0.1 86,537 - -0.2 86,538 + +0.1 86,539 - -0.2 86,539 - +0.7 86,539 - -0.2 86,547 - +1.1 86,592 + -0.9 86,592 + -1.1 86,593 - -0.9 86,593 - -0.1 86,615 - -0.4 86,622 - +0.2 86,627 + +0.2 86,627 + +0.2 86,699 + -1.9 86,722 - -2.7 86,752 - EX28DRAFT_0142 0.25 +0.1 86,760 + EX28DRAFT_0142 0.30 +0.3 86,761 - EX28DRAFT_0142 0.31 -1.0 86,795 - EX28DRAFT_0142 0.50 -0.8 86,849 + EX28DRAFT_0142 0.80 -0.7 86,849 + EX28DRAFT_0142 0.80 -0.1 86,850 - EX28DRAFT_0142 0.81 -1.0 86,865 + EX28DRAFT_0142 0.89 -0.6 86,941 + -1.1 86,986 - -1.3 86,986 - -0.1 86,998 + -0.6 87,018 - -0.7 87,035 - +1.0 87,035 - -0.4 87,038 + +0.0 87,038 + +1.8 87,050 - +1.3 87,083 - -0.1 87,217 - -0.3 87,530 + +0.2 87,585 - -0.4 87,702 - -0.6 87,702 - +0.3 87,702 - -0.1 87,702 - -0.5 87,804 + +0.3 87,805 - +0.1
Or see this region's nucleotide sequence