Experiment: Nickel (II) chloride 80 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_24920 and IAI46_24925 are separated by 39 nucleotides IAI46_24925 and IAI46_24930 are separated by 23 nucleotides IAI46_24930 and IAI46_24935 overlap by 4 nucleotides
IAI46_24920: IAI46_24920 - AsmA family protein, at 5,317,461 to 5,319,122
_24920
IAI46_24925: IAI46_24925 - fatty acid biosynthesis protein FabY, at 5,319,162 to 5,320,109
_24925
IAI46_24930: IAI46_24930 - D-tyrosyl-tRNA(Tyr) deacylase, at 5,320,133 to 5,320,570
_24930
IAI46_24935: IAI46_24935 - virulence factor BrkB family protein, at 5,320,567 to 5,321,457
_24935
Position (kb) 5319
5320
5321 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5318.195 kb on + strand, within IAI46_24920 at 5318.237 kb on - strand, within IAI46_24920 at 5318.237 kb on - strand, within IAI46_24920 at 5318.270 kb on + strand, within IAI46_24920 at 5318.270 kb on + strand, within IAI46_24920 at 5318.271 kb on - strand, within IAI46_24920 at 5318.349 kb on - strand, within IAI46_24920 at 5318.402 kb on + strand, within IAI46_24920 at 5318.403 kb on - strand, within IAI46_24920 at 5318.403 kb on - strand, within IAI46_24920 at 5318.403 kb on - strand, within IAI46_24920 at 5318.464 kb on + strand, within IAI46_24920 at 5318.464 kb on + strand, within IAI46_24920 at 5318.464 kb on + strand, within IAI46_24920 at 5318.541 kb on + strand, within IAI46_24920 at 5318.542 kb on - strand, within IAI46_24920 at 5318.542 kb on - strand, within IAI46_24920 at 5318.543 kb on + strand, within IAI46_24920 at 5318.543 kb on + strand, within IAI46_24920 at 5318.544 kb on - strand, within IAI46_24920 at 5318.544 kb on - strand, within IAI46_24920 at 5318.544 kb on - strand, within IAI46_24920 at 5318.544 kb on - strand, within IAI46_24920 at 5318.552 kb on + strand, within IAI46_24920 at 5318.552 kb on + strand, within IAI46_24920 at 5318.553 kb on - strand, within IAI46_24920 at 5318.554 kb on + strand, within IAI46_24920 at 5318.555 kb on - strand, within IAI46_24920 at 5318.555 kb on - strand, within IAI46_24920 at 5318.575 kb on + strand, within IAI46_24920 at 5318.575 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.607 kb on + strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.608 kb on - strand, within IAI46_24920 at 5318.615 kb on + strand, within IAI46_24920 at 5318.616 kb on - strand, within IAI46_24920 at 5318.649 kb on - strand, within IAI46_24920 at 5318.771 kb on + strand, within IAI46_24920 at 5318.771 kb on + strand, within IAI46_24920 at 5318.771 kb on + strand, within IAI46_24920 at 5318.772 kb on - strand, within IAI46_24920 at 5318.772 kb on - strand, within IAI46_24920 at 5318.772 kb on - strand, within IAI46_24920 at 5318.859 kb on - strand, within IAI46_24920 at 5318.918 kb on + strand, within IAI46_24920 at 5318.919 kb on - strand, within IAI46_24920 at 5318.919 kb on - strand, within IAI46_24920 at 5318.955 kb on - strand, within IAI46_24920 at 5318.955 kb on - strand, within IAI46_24920 at 5318.959 kb on + strand at 5318.959 kb on + strand at 5318.960 kb on - strand at 5318.960 kb on - strand at 5319.013 kb on + strand at 5319.014 kb on - strand at 5319.014 kb on - strand at 5319.050 kb on + strand at 5319.050 kb on + strand at 5319.050 kb on + strand at 5319.051 kb on - strand at 5319.051 kb on - strand at 5319.120 kb on + strand at 5319.120 kb on + strand at 5319.131 kb on - strand at 5319.145 kb on + strand at 5319.145 kb on + strand at 5319.145 kb on + strand at 5319.146 kb on - strand at 5319.163 kb on + strand at 5319.163 kb on + strand at 5319.163 kb on + strand at 5319.163 kb on + strand at 5319.163 kb on + strand at 5319.163 kb on + strand at 5319.164 kb on - strand at 5319.164 kb on - strand at 5319.227 kb on + strand at 5319.227 kb on + strand at 5319.227 kb on + strand at 5319.227 kb on + strand at 5319.227 kb on + strand at 5319.227 kb on + strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.228 kb on - strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.229 kb on + strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.230 kb on - strand at 5319.243 kb on + strand at 5319.243 kb on + strand at 5319.244 kb on - strand at 5319.244 kb on - strand at 5319.565 kb on + strand, within IAI46_24925 at 5319.566 kb on - strand, within IAI46_24925 at 5319.613 kb on + strand, within IAI46_24925 at 5319.614 kb on - strand, within IAI46_24925 at 5319.740 kb on + strand, within IAI46_24925 at 5319.741 kb on - strand, within IAI46_24925 at 5319.789 kb on - strand, within IAI46_24925 at 5319.795 kb on + strand, within IAI46_24925 at 5319.796 kb on - strand, within IAI46_24925 at 5319.796 kb on - strand, within IAI46_24925 at 5319.882 kb on + strand, within IAI46_24925 at 5319.926 kb on - strand, within IAI46_24925 at 5319.926 kb on - strand, within IAI46_24925 at 5319.926 kb on - strand, within IAI46_24925 at 5319.926 kb on - strand, within IAI46_24925 at 5319.926 kb on - strand, within IAI46_24925 at 5319.994 kb on + strand, within IAI46_24925 at 5319.994 kb on + strand, within IAI46_24925 at 5320.060 kb on + strand at 5320.061 kb on - strand at 5320.063 kb on + strand at 5320.063 kb on + strand at 5320.063 kb on + strand at 5320.064 kb on - strand at 5320.064 kb on - strand at 5320.093 kb on - strand at 5320.098 kb on + strand at 5320.098 kb on + strand at 5320.105 kb on + strand at 5320.105 kb on + strand at 5320.105 kb on + strand at 5320.106 kb on - strand at 5320.106 kb on - strand at 5320.106 kb on - strand at 5320.109 kb on + strand at 5320.109 kb on + strand at 5320.109 kb on + strand at 5320.109 kb on + strand at 5320.109 kb on + strand at 5320.109 kb on + strand at 5320.109 kb on + strand at 5320.109 kb on + strand at 5320.109 kb on + strand at 5320.110 kb on - strand at 5320.110 kb on - strand at 5320.110 kb on - strand at 5320.110 kb on - strand at 5320.110 kb on - strand at 5320.110 kb on - strand at 5320.248 kb on + strand, within IAI46_24930 at 5320.254 kb on + strand, within IAI46_24930 at 5320.255 kb on - strand, within IAI46_24930 at 5320.255 kb on - strand, within IAI46_24930 at 5320.255 kb on - strand, within IAI46_24930 at 5320.255 kb on - strand, within IAI46_24930 at 5320.257 kb on - strand, within IAI46_24930 at 5320.481 kb on + strand, within IAI46_24930 at 5320.481 kb on + strand, within IAI46_24930 at 5320.488 kb on - strand, within IAI46_24930 at 5320.502 kb on + strand, within IAI46_24930 at 5320.502 kb on + strand, within IAI46_24930 at 5320.502 kb on + strand, within IAI46_24930 at 5320.502 kb on + strand, within IAI46_24930 at 5320.503 kb on - strand, within IAI46_24930 at 5320.503 kb on - strand, within IAI46_24930 at 5320.503 kb on - strand, within IAI46_24930 at 5320.503 kb on - strand, within IAI46_24930 at 5320.503 kb on - strand, within IAI46_24930 at 5320.503 kb on - strand, within IAI46_24930 at 5320.512 kb on - strand, within IAI46_24930 at 5320.544 kb on + strand at 5320.545 kb on - strand at 5320.545 kb on - strand at 5320.545 kb on - strand at 5320.545 kb on - strand at 5320.545 kb on - strand at 5320.547 kb on + strand at 5320.547 kb on + strand at 5320.560 kb on - strand at 5320.671 kb on + strand, within IAI46_24935 at 5320.671 kb on + strand, within IAI46_24935 at 5320.671 kb on + strand, within IAI46_24935 at 5320.699 kb on + strand, within IAI46_24935 at 5320.727 kb on + strand, within IAI46_24935 at 5320.739 kb on + strand, within IAI46_24935 at 5320.740 kb on - strand, within IAI46_24935 at 5320.760 kb on + strand, within IAI46_24935 at 5320.760 kb on + strand, within IAI46_24935 at 5320.760 kb on + strand, within IAI46_24935 at 5320.760 kb on + strand, within IAI46_24935 at 5320.760 kb on + strand, within IAI46_24935 at 5320.761 kb on - strand, within IAI46_24935 at 5320.843 kb on - strand, within IAI46_24935 at 5320.862 kb on + strand, within IAI46_24935 at 5320.862 kb on + strand, within IAI46_24935 at 5320.862 kb on + strand, within IAI46_24935 at 5320.956 kb on + strand, within IAI46_24935 at 5320.956 kb on + strand, within IAI46_24935 at 5320.956 kb on + strand, within IAI46_24935 at 5320.970 kb on + strand, within IAI46_24935 at 5320.987 kb on - strand, within IAI46_24935 at 5321.030 kb on + strand, within IAI46_24935 at 5321.053 kb on - strand, within IAI46_24935 at 5321.053 kb on - strand, within IAI46_24935
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, anaerobic remove 5,318,195 + IAI46_24920 0.44 +1.4 5,318,237 - IAI46_24920 0.47 +0.9 5,318,237 - IAI46_24920 0.47 +1.4 5,318,270 + IAI46_24920 0.49 -0.1 5,318,270 + IAI46_24920 0.49 -1.1 5,318,271 - IAI46_24920 0.49 -0.5 5,318,349 - IAI46_24920 0.53 -0.5 5,318,402 + IAI46_24920 0.57 +0.7 5,318,403 - IAI46_24920 0.57 +1.5 5,318,403 - IAI46_24920 0.57 -0.2 5,318,403 - IAI46_24920 0.57 +0.0 5,318,464 + IAI46_24920 0.60 +1.1 5,318,464 + IAI46_24920 0.60 +0.1 5,318,464 + IAI46_24920 0.60 -0.3 5,318,541 + IAI46_24920 0.65 -0.3 5,318,542 - IAI46_24920 0.65 -0.6 5,318,542 - IAI46_24920 0.65 -0.7 5,318,543 + IAI46_24920 0.65 -0.2 5,318,543 + IAI46_24920 0.65 -0.1 5,318,544 - IAI46_24920 0.65 -0.1 5,318,544 - IAI46_24920 0.65 -1.9 5,318,544 - IAI46_24920 0.65 +0.7 5,318,544 - IAI46_24920 0.65 +0.1 5,318,552 + IAI46_24920 0.66 +0.3 5,318,552 + IAI46_24920 0.66 +0.7 5,318,553 - IAI46_24920 0.66 +0.3 5,318,554 + IAI46_24920 0.66 +0.2 5,318,555 - IAI46_24920 0.66 -0.3 5,318,555 - IAI46_24920 0.66 +0.5 5,318,575 + IAI46_24920 0.67 -0.4 5,318,575 + IAI46_24920 0.67 +0.3 5,318,607 + IAI46_24920 0.69 -1.0 5,318,607 + IAI46_24920 0.69 -0.5 5,318,607 + IAI46_24920 0.69 -1.8 5,318,607 + IAI46_24920 0.69 +0.0 5,318,607 + IAI46_24920 0.69 -0.1 5,318,607 + IAI46_24920 0.69 +0.5 5,318,607 + IAI46_24920 0.69 -1.2 5,318,607 + IAI46_24920 0.69 -0.1 5,318,607 + IAI46_24920 0.69 +0.8 5,318,608 - IAI46_24920 0.69 +0.1 5,318,608 - IAI46_24920 0.69 +0.1 5,318,608 - IAI46_24920 0.69 -0.2 5,318,608 - IAI46_24920 0.69 +0.1 5,318,608 - IAI46_24920 0.69 -0.2 5,318,608 - IAI46_24920 0.69 +0.1 5,318,608 - IAI46_24920 0.69 +0.5 5,318,608 - IAI46_24920 0.69 +0.3 5,318,608 - IAI46_24920 0.69 +1.1 5,318,615 + IAI46_24920 0.69 +0.1 5,318,616 - IAI46_24920 0.69 +0.8 5,318,649 - IAI46_24920 0.71 +0.2 5,318,771 + IAI46_24920 0.79 -0.5 5,318,771 + IAI46_24920 0.79 +0.3 5,318,771 + IAI46_24920 0.79 +0.8 5,318,772 - IAI46_24920 0.79 -0.3 5,318,772 - IAI46_24920 0.79 -1.2 5,318,772 - IAI46_24920 0.79 +0.0 5,318,859 - IAI46_24920 0.84 +0.1 5,318,918 + IAI46_24920 0.88 -0.5 5,318,919 - IAI46_24920 0.88 +0.0 5,318,919 - IAI46_24920 0.88 -0.2 5,318,955 - IAI46_24920 0.90 -1.1 5,318,955 - IAI46_24920 0.90 -0.1 5,318,959 + +0.2 5,318,959 + -0.2 5,318,960 - +0.9 5,318,960 - -0.6 5,319,013 + +1.3 5,319,014 - +0.2 5,319,014 - -0.5 5,319,050 + -1.1 5,319,050 + -0.0 5,319,050 + +0.8 5,319,051 - -0.9 5,319,051 - -0.1 5,319,120 + -0.6 5,319,120 + -0.1 5,319,131 - +0.9 5,319,145 + -0.9 5,319,145 + -1.0 5,319,145 + +0.2 5,319,146 - +0.4 5,319,163 + +0.1 5,319,163 + -0.7 5,319,163 + -0.6 5,319,163 + +0.1 5,319,163 + -1.0 5,319,163 + -0.4 5,319,164 - -0.3 5,319,164 - -0.1 5,319,227 + -0.8 5,319,227 + +0.1 5,319,227 + +0.2 5,319,227 + +0.2 5,319,227 + -0.4 5,319,227 + -0.8 5,319,228 - -0.2 5,319,228 - +0.6 5,319,228 - +1.0 5,319,228 - +0.3 5,319,228 - +1.1 5,319,228 - -0.4 5,319,228 - +0.2 5,319,228 - +1.7 5,319,228 - -0.4 5,319,228 - +0.6 5,319,229 + -0.5 5,319,229 + +0.7 5,319,229 + +0.6 5,319,229 + -1.7 5,319,229 + -1.4 5,319,229 + +0.5 5,319,229 + +0.3 5,319,229 + +0.2 5,319,229 + +0.7 5,319,229 + +0.2 5,319,229 + +0.1 5,319,229 + +0.3 5,319,229 + -0.3 5,319,229 + -1.7 5,319,230 - +0.0 5,319,230 - +0.1 5,319,230 - -0.7 5,319,230 - -0.1 5,319,230 - +0.3 5,319,230 - +0.1 5,319,230 - +0.6 5,319,230 - +0.2 5,319,230 - -0.1 5,319,230 - +0.5 5,319,230 - +0.7 5,319,230 - -0.7 5,319,230 - +0.0 5,319,243 + -1.1 5,319,243 + -0.1 5,319,244 - -0.4 5,319,244 - +0.7 5,319,565 + IAI46_24925 0.43 +0.4 5,319,566 - IAI46_24925 0.43 +1.7 5,319,613 + IAI46_24925 0.48 -0.3 5,319,614 - IAI46_24925 0.48 +0.7 5,319,740 + IAI46_24925 0.61 +1.0 5,319,741 - IAI46_24925 0.61 -0.7 5,319,789 - IAI46_24925 0.66 +0.9 5,319,795 + IAI46_24925 0.67 +0.3 5,319,796 - IAI46_24925 0.67 -0.2 5,319,796 - IAI46_24925 0.67 -1.2 5,319,882 + IAI46_24925 0.76 -0.1 5,319,926 - IAI46_24925 0.81 +0.3 5,319,926 - IAI46_24925 0.81 +0.3 5,319,926 - IAI46_24925 0.81 +1.1 5,319,926 - IAI46_24925 0.81 +1.9 5,319,926 - IAI46_24925 0.81 +0.9 5,319,994 + IAI46_24925 0.88 -0.4 5,319,994 + IAI46_24925 0.88 +0.3 5,320,060 + +1.3 5,320,061 - -0.0 5,320,063 + -0.3 5,320,063 + -0.1 5,320,063 + +0.5 5,320,064 - -1.3 5,320,064 - +0.2 5,320,093 - +0.8 5,320,098 + -1.1 5,320,098 + -0.5 5,320,105 + +0.5 5,320,105 + +0.7 5,320,105 + -0.2 5,320,106 - -0.9 5,320,106 - +0.1 5,320,106 - +0.1 5,320,109 + +0.5 5,320,109 + +1.1 5,320,109 + +0.1 5,320,109 + +0.7 5,320,109 + -0.2 5,320,109 + +0.8 5,320,109 + +0.1 5,320,109 + -0.0 5,320,109 + +0.3 5,320,110 - +0.5 5,320,110 - +0.7 5,320,110 - +0.2 5,320,110 - +0.3 5,320,110 - +0.5 5,320,110 - -0.8 5,320,248 + IAI46_24930 0.26 -0.5 5,320,254 + IAI46_24930 0.28 +0.0 5,320,255 - IAI46_24930 0.28 -0.0 5,320,255 - IAI46_24930 0.28 -0.4 5,320,255 - IAI46_24930 0.28 +0.7 5,320,255 - IAI46_24930 0.28 -0.0 5,320,257 - IAI46_24930 0.28 +0.0 5,320,481 + IAI46_24930 0.79 -0.4 5,320,481 + IAI46_24930 0.79 -0.0 5,320,488 - IAI46_24930 0.81 +0.3 5,320,502 + IAI46_24930 0.84 +0.3 5,320,502 + IAI46_24930 0.84 -1.9 5,320,502 + IAI46_24930 0.84 -0.9 5,320,502 + IAI46_24930 0.84 +0.4 5,320,503 - IAI46_24930 0.84 +0.3 5,320,503 - IAI46_24930 0.84 +0.3 5,320,503 - IAI46_24930 0.84 -0.8 5,320,503 - IAI46_24930 0.84 +0.2 5,320,503 - IAI46_24930 0.84 -0.5 5,320,503 - IAI46_24930 0.84 -0.1 5,320,512 - IAI46_24930 0.87 +0.5 5,320,544 + +1.1 5,320,545 - -0.9 5,320,545 - -0.3 5,320,545 - +0.5 5,320,545 - +0.6 5,320,545 - +0.9 5,320,547 + -1.9 5,320,547 + -0.4 5,320,560 - +1.5 5,320,671 + IAI46_24935 0.12 +0.8 5,320,671 + IAI46_24935 0.12 +1.1 5,320,671 + IAI46_24935 0.12 +1.0 5,320,699 + IAI46_24935 0.15 +0.3 5,320,727 + IAI46_24935 0.18 -0.3 5,320,739 + IAI46_24935 0.19 -1.7 5,320,740 - IAI46_24935 0.19 +0.7 5,320,760 + IAI46_24935 0.22 -0.3 5,320,760 + IAI46_24935 0.22 -0.2 5,320,760 + IAI46_24935 0.22 -0.0 5,320,760 + IAI46_24935 0.22 +0.2 5,320,760 + IAI46_24935 0.22 -0.6 5,320,761 - IAI46_24935 0.22 +0.1 5,320,843 - IAI46_24935 0.31 -0.3 5,320,862 + IAI46_24935 0.33 +0.8 5,320,862 + IAI46_24935 0.33 -0.1 5,320,862 + IAI46_24935 0.33 -0.3 5,320,956 + IAI46_24935 0.44 +0.4 5,320,956 + IAI46_24935 0.44 +0.4 5,320,956 + IAI46_24935 0.44 +0.1 5,320,970 + IAI46_24935 0.45 -0.7 5,320,987 - IAI46_24935 0.47 +0.4 5,321,030 + IAI46_24935 0.52 -0.4 5,321,053 - IAI46_24935 0.55 -0.4 5,321,053 - IAI46_24935 0.55 +0.5
Or see this region's nucleotide sequence