Experiment: Nickel (II) chloride 80 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_24830 and IAI46_24835 overlap by 23 nucleotides IAI46_24835 and IAI46_24840 are separated by 260 nucleotides IAI46_24840 and IAI46_24845 are separated by 47 nucleotides
IAI46_24830: IAI46_24830 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 5,297,779 to 5,298,996
_24830
IAI46_24835: IAI46_24835 - dUTP diphosphatase, at 5,298,974 to 5,299,432
_24835
IAI46_24840: IAI46_24840 - nucleoid occlusion factor SlmA, at 5,299,693 to 5,300,289
_24840
IAI46_24845: IAI46_24845 - orotate phosphoribosyltransferase, at 5,300,337 to 5,300,978
_24845
Position (kb) 5298
5299
5300 Strain fitness (log2 ratio) -1
0
1
2 at 5299.430 kb on + strand at 5299.444 kb on + strand at 5299.484 kb on + strand at 5299.527 kb on + strand at 5299.527 kb on + strand at 5299.527 kb on + strand at 5299.533 kb on + strand at 5299.533 kb on + strand at 5299.533 kb on + strand at 5299.534 kb on - strand at 5299.562 kb on - strand at 5299.562 kb on - strand at 5299.571 kb on + strand at 5299.603 kb on + strand at 5299.603 kb on + strand at 5299.604 kb on - strand at 5299.610 kb on + strand at 5299.610 kb on + strand at 5299.610 kb on + strand at 5299.611 kb on - strand at 5299.611 kb on - strand at 5299.611 kb on - strand at 5299.664 kb on - strand at 5299.673 kb on + strand at 5299.684 kb on + strand at 5299.684 kb on + strand at 5299.684 kb on + strand at 5299.711 kb on - strand at 5299.785 kb on + strand, within IAI46_24840 at 5299.785 kb on + strand, within IAI46_24840 at 5299.786 kb on - strand, within IAI46_24840 at 5299.786 kb on - strand, within IAI46_24840 at 5299.788 kb on + strand, within IAI46_24840 at 5299.789 kb on - strand, within IAI46_24840 at 5299.838 kb on - strand, within IAI46_24840 at 5299.888 kb on - strand, within IAI46_24840 at 5299.901 kb on + strand, within IAI46_24840 at 5299.902 kb on - strand, within IAI46_24840 at 5299.977 kb on - strand, within IAI46_24840 at 5300.145 kb on + strand, within IAI46_24840 at 5300.210 kb on - strand, within IAI46_24840 at 5300.210 kb on - strand, within IAI46_24840 at 5300.287 kb on + strand at 5300.287 kb on + strand at 5300.310 kb on + strand at 5300.311 kb on - strand at 5300.311 kb on - strand at 5300.311 kb on - strand at 5300.326 kb on + strand at 5300.326 kb on + strand at 5300.326 kb on + strand at 5300.326 kb on + strand at 5300.327 kb on - strand at 5300.327 kb on - strand at 5300.327 kb on - strand at 5300.330 kb on + strand at 5300.331 kb on - strand at 5300.331 kb on - strand at 5300.331 kb on - strand at 5300.348 kb on - strand at 5300.410 kb on + strand, within IAI46_24845 at 5300.411 kb on - strand, within IAI46_24845 at 5300.422 kb on + strand, within IAI46_24845
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, anaerobic remove 5,299,430 + +0.3 5,299,444 + -0.3 5,299,484 + +0.6 5,299,527 + -0.7 5,299,527 + -0.2 5,299,527 + +0.8 5,299,533 + -0.5 5,299,533 + -0.4 5,299,533 + -0.5 5,299,534 - -0.3 5,299,562 - -0.9 5,299,562 - -0.5 5,299,571 + -0.5 5,299,603 + +1.2 5,299,603 + +0.2 5,299,604 - +0.4 5,299,610 + +0.8 5,299,610 + +0.2 5,299,610 + -0.0 5,299,611 - -0.1 5,299,611 - -1.2 5,299,611 - +0.2 5,299,664 - +1.2 5,299,673 + +0.2 5,299,684 + -0.5 5,299,684 + -0.9 5,299,684 + -0.2 5,299,711 - +0.5 5,299,785 + IAI46_24840 0.15 -1.1 5,299,785 + IAI46_24840 0.15 +0.2 5,299,786 - IAI46_24840 0.16 -0.3 5,299,786 - IAI46_24840 0.16 +1.1 5,299,788 + IAI46_24840 0.16 -0.2 5,299,789 - IAI46_24840 0.16 -0.7 5,299,838 - IAI46_24840 0.24 +2.0 5,299,888 - IAI46_24840 0.33 +0.3 5,299,901 + IAI46_24840 0.35 +1.1 5,299,902 - IAI46_24840 0.35 -0.1 5,299,977 - IAI46_24840 0.48 +0.6 5,300,145 + IAI46_24840 0.76 -1.3 5,300,210 - IAI46_24840 0.87 -1.1 5,300,210 - IAI46_24840 0.87 +0.5 5,300,287 + +1.1 5,300,287 + -0.8 5,300,310 + +0.5 5,300,311 - -0.4 5,300,311 - -0.2 5,300,311 - -0.3 5,300,326 + +0.2 5,300,326 + -0.3 5,300,326 + +1.2 5,300,326 + +0.6 5,300,327 - +0.6 5,300,327 - -0.1 5,300,327 - +1.3 5,300,330 + -0.3 5,300,331 - -1.2 5,300,331 - +0.2 5,300,331 - -0.1 5,300,348 - -0.3 5,300,410 + IAI46_24845 0.11 +0.8 5,300,411 - IAI46_24845 0.12 +0.7 5,300,422 + IAI46_24845 0.13 +1.8
Or see this region's nucleotide sequence