Experiment: Nickel (II) chloride 80 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_17160 and IAI46_17165 are separated by 394 nucleotides IAI46_17165 and IAI46_17170 are separated by 20 nucleotides
IAI46_17160: IAI46_17160 - glutamine synthetase, at 3,598,434 to 3,599,852
_17160
IAI46_17165: IAI46_17165 - HTH-type transcriptional regulator PuuR, at 3,600,247 to 3,600,804
_17165
IAI46_17170: IAI46_17170 - FAD-binding oxidoreductase, at 3,600,825 to 3,602,105
_17170
Position (kb)
3600
3601 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3599.292 kb on - strand, within IAI46_17160 at 3599.365 kb on + strand, within IAI46_17160 at 3599.365 kb on + strand, within IAI46_17160 at 3599.365 kb on + strand, within IAI46_17160 at 3599.366 kb on - strand, within IAI46_17160 at 3599.386 kb on - strand, within IAI46_17160 at 3599.442 kb on - strand, within IAI46_17160 at 3599.552 kb on + strand, within IAI46_17160 at 3599.618 kb on + strand, within IAI46_17160 at 3599.647 kb on + strand, within IAI46_17160 at 3599.647 kb on + strand, within IAI46_17160 at 3599.648 kb on - strand, within IAI46_17160 at 3599.648 kb on - strand, within IAI46_17160 at 3599.735 kb on + strand at 3599.738 kb on - strand at 3599.765 kb on - strand at 3599.792 kb on + strand at 3599.793 kb on - strand at 3599.810 kb on + strand at 3599.852 kb on + strand at 3599.852 kb on + strand at 3599.852 kb on + strand at 3599.852 kb on + strand at 3599.853 kb on - strand at 3599.867 kb on + strand at 3599.867 kb on + strand at 3599.932 kb on - strand at 3599.972 kb on - strand at 3600.009 kb on + strand at 3600.055 kb on + strand at 3600.154 kb on + strand at 3600.241 kb on - strand at 3600.246 kb on + strand at 3600.376 kb on - strand, within IAI46_17165 at 3600.376 kb on - strand, within IAI46_17165 at 3600.485 kb on + strand, within IAI46_17165 at 3600.485 kb on + strand, within IAI46_17165 at 3600.486 kb on - strand, within IAI46_17165 at 3600.509 kb on + strand, within IAI46_17165 at 3600.712 kb on + strand, within IAI46_17165 at 3600.712 kb on + strand, within IAI46_17165 at 3600.713 kb on - strand, within IAI46_17165 at 3600.750 kb on + strand at 3600.750 kb on + strand at 3600.751 kb on - strand at 3600.787 kb on - strand at 3600.796 kb on - strand at 3600.847 kb on - strand at 3600.847 kb on - strand at 3600.849 kb on + strand at 3600.850 kb on - strand at 3600.988 kb on + strand, within IAI46_17170 at 3601.042 kb on + strand, within IAI46_17170 at 3601.042 kb on + strand, within IAI46_17170 at 3601.054 kb on - strand, within IAI46_17170 at 3601.087 kb on - strand, within IAI46_17170 at 3601.164 kb on + strand, within IAI46_17170 at 3601.183 kb on - strand, within IAI46_17170 at 3601.264 kb on - strand, within IAI46_17170 at 3601.302 kb on - strand, within IAI46_17170 at 3601.330 kb on - strand, within IAI46_17170 at 3601.622 kb on + strand, within IAI46_17170 at 3601.623 kb on - strand, within IAI46_17170 at 3601.683 kb on + strand, within IAI46_17170 at 3601.684 kb on - strand, within IAI46_17170 at 3601.733 kb on - strand, within IAI46_17170 at 3601.733 kb on - strand, within IAI46_17170 at 3601.734 kb on + strand, within IAI46_17170
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, anaerobic remove 3,599,292 - IAI46_17160 0.60 +0.0 3,599,365 + IAI46_17160 0.66 +0.8 3,599,365 + IAI46_17160 0.66 -2.1 3,599,365 + IAI46_17160 0.66 +0.6 3,599,366 - IAI46_17160 0.66 -0.2 3,599,386 - IAI46_17160 0.67 +0.7 3,599,442 - IAI46_17160 0.71 -0.4 3,599,552 + IAI46_17160 0.79 -1.7 3,599,618 + IAI46_17160 0.83 +1.8 3,599,647 + IAI46_17160 0.85 +0.1 3,599,647 + IAI46_17160 0.85 +0.1 3,599,648 - IAI46_17160 0.86 -1.6 3,599,648 - IAI46_17160 0.86 +0.2 3,599,735 + +2.8 3,599,738 - +1.2 3,599,765 - +0.7 3,599,792 + +0.4 3,599,793 - +0.2 3,599,810 + -0.0 3,599,852 + -1.5 3,599,852 + -1.1 3,599,852 + +0.1 3,599,852 + -0.9 3,599,853 - +0.5 3,599,867 + -0.4 3,599,867 + -0.2 3,599,932 - -0.3 3,599,972 - -0.1 3,600,009 + +0.4 3,600,055 + +0.4 3,600,154 + +0.0 3,600,241 - -0.5 3,600,246 + +1.4 3,600,376 - IAI46_17165 0.23 +0.4 3,600,376 - IAI46_17165 0.23 +0.9 3,600,485 + IAI46_17165 0.43 +0.3 3,600,485 + IAI46_17165 0.43 +0.6 3,600,486 - IAI46_17165 0.43 -0.5 3,600,509 + IAI46_17165 0.47 +1.0 3,600,712 + IAI46_17165 0.83 -0.8 3,600,712 + IAI46_17165 0.83 -0.1 3,600,713 - IAI46_17165 0.84 -0.9 3,600,750 + -1.3 3,600,750 + +0.0 3,600,751 - -0.4 3,600,787 - -0.5 3,600,796 - +0.4 3,600,847 - +0.2 3,600,847 - -0.5 3,600,849 + -0.5 3,600,850 - +0.5 3,600,988 + IAI46_17170 0.13 +1.5 3,601,042 + IAI46_17170 0.17 -0.0 3,601,042 + IAI46_17170 0.17 -0.2 3,601,054 - IAI46_17170 0.18 -1.9 3,601,087 - IAI46_17170 0.20 +0.9 3,601,164 + IAI46_17170 0.26 -0.4 3,601,183 - IAI46_17170 0.28 -1.0 3,601,264 - IAI46_17170 0.34 -0.4 3,601,302 - IAI46_17170 0.37 +1.4 3,601,330 - IAI46_17170 0.39 -0.5 3,601,622 + IAI46_17170 0.62 +0.5 3,601,623 - IAI46_17170 0.62 -1.7 3,601,683 + IAI46_17170 0.67 +0.3 3,601,684 - IAI46_17170 0.67 -0.1 3,601,733 - IAI46_17170 0.71 +0.8 3,601,733 - IAI46_17170 0.71 +0.6 3,601,734 + IAI46_17170 0.71 -0.4
Or see this region's nucleotide sequence