Experiment: Nickel (II) chloride 80 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_09710 and IAI46_09715 are separated by 133 nucleotides IAI46_09715 and IAI46_09720 are separated by 225 nucleotides IAI46_09720 and IAI46_09725 are separated by 143 nucleotides IAI46_09725 and IAI46_09730 are separated by 199 nucleotides
IAI46_09710: IAI46_09710 - DUF2770 family protein, at 2,059,206 to 2,059,322
_09710
IAI46_09715: IAI46_09715 - helix-turn-helix transcriptional regulator, at 2,059,456 to 2,060,082
_09715
IAI46_09720: IAI46_09720 - N-methyl-L-tryptophan oxidase, at 2,060,308 to 2,061,423
_09720
IAI46_09725: IAI46_09725 - biofilm formation regulator BssS, at 2,061,567 to 2,061,821
_09725
IAI46_09730: IAI46_09730 - hypothetical protein, at 2,062,021 to 2,062,299
_09730
Position (kb)
2060
2061
2062 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2059.308 kb on + strand, within IAI46_09710 at 2059.340 kb on + strand at 2059.356 kb on - strand at 2059.379 kb on - strand at 2059.381 kb on + strand at 2059.458 kb on - strand at 2059.460 kb on - strand at 2059.460 kb on - strand at 2059.482 kb on + strand at 2059.483 kb on - strand at 2059.528 kb on - strand, within IAI46_09715 at 2059.530 kb on - strand, within IAI46_09715 at 2059.555 kb on - strand, within IAI46_09715 at 2059.595 kb on + strand, within IAI46_09715 at 2059.637 kb on + strand, within IAI46_09715 at 2059.845 kb on + strand, within IAI46_09715 at 2059.846 kb on - strand, within IAI46_09715 at 2059.846 kb on - strand, within IAI46_09715 at 2059.876 kb on - strand, within IAI46_09715 at 2059.999 kb on + strand, within IAI46_09715 at 2060.120 kb on + strand at 2060.142 kb on - strand at 2060.314 kb on - strand at 2060.314 kb on - strand at 2060.340 kb on + strand at 2060.340 kb on + strand at 2060.341 kb on - strand at 2060.345 kb on - strand at 2060.345 kb on - strand at 2060.389 kb on + strand at 2060.493 kb on + strand, within IAI46_09720 at 2060.494 kb on - strand, within IAI46_09720 at 2060.498 kb on + strand, within IAI46_09720 at 2060.499 kb on - strand, within IAI46_09720 at 2060.499 kb on - strand, within IAI46_09720 at 2060.499 kb on - strand, within IAI46_09720 at 2060.499 kb on - strand, within IAI46_09720 at 2060.518 kb on + strand, within IAI46_09720 at 2060.684 kb on + strand, within IAI46_09720 at 2060.684 kb on + strand, within IAI46_09720 at 2060.685 kb on - strand, within IAI46_09720 at 2060.735 kb on - strand, within IAI46_09720 at 2060.771 kb on - strand, within IAI46_09720 at 2061.263 kb on + strand, within IAI46_09720 at 2061.331 kb on + strand at 2061.337 kb on + strand at 2061.338 kb on - strand at 2061.366 kb on - strand at 2061.368 kb on + strand at 2061.409 kb on + strand at 2061.409 kb on + strand at 2061.410 kb on - strand at 2061.417 kb on - strand at 2061.430 kb on + strand at 2061.443 kb on - strand at 2061.467 kb on - strand at 2061.568 kb on + strand at 2061.569 kb on - strand at 2061.685 kb on - strand, within IAI46_09725 at 2061.685 kb on - strand, within IAI46_09725 at 2061.725 kb on - strand, within IAI46_09725 at 2061.735 kb on + strand, within IAI46_09725 at 2061.736 kb on - strand, within IAI46_09725 at 2061.751 kb on + strand, within IAI46_09725 at 2061.765 kb on - strand, within IAI46_09725 at 2061.770 kb on + strand, within IAI46_09725 at 2061.801 kb on + strand at 2061.839 kb on + strand at 2061.842 kb on + strand at 2061.842 kb on + strand at 2061.843 kb on - strand at 2061.843 kb on - strand at 2061.846 kb on + strand at 2061.846 kb on + strand at 2061.847 kb on - strand at 2061.897 kb on + strand at 2061.898 kb on - strand at 2061.898 kb on - strand at 2061.902 kb on + strand at 2061.903 kb on - strand at 2061.905 kb on - strand at 2061.996 kb on + strand at 2061.997 kb on - strand at 2062.000 kb on + strand at 2062.000 kb on + strand at 2062.000 kb on + strand at 2062.000 kb on + strand at 2062.029 kb on + strand at 2062.153 kb on + strand, within IAI46_09730 at 2062.153 kb on + strand, within IAI46_09730 at 2062.153 kb on + strand, within IAI46_09730 at 2062.210 kb on + strand, within IAI46_09730 at 2062.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, anaerobic remove 2,059,308 + IAI46_09710 0.87 -0.3 2,059,340 + +1.8 2,059,356 - -0.1 2,059,379 - +0.2 2,059,381 + +1.2 2,059,458 - -0.1 2,059,460 - +0.1 2,059,460 - -1.0 2,059,482 + -0.3 2,059,483 - +1.0 2,059,528 - IAI46_09715 0.11 -0.3 2,059,530 - IAI46_09715 0.12 +0.2 2,059,555 - IAI46_09715 0.16 +1.3 2,059,595 + IAI46_09715 0.22 -1.6 2,059,637 + IAI46_09715 0.29 +1.2 2,059,845 + IAI46_09715 0.62 +0.2 2,059,846 - IAI46_09715 0.62 -0.4 2,059,846 - IAI46_09715 0.62 -2.1 2,059,876 - IAI46_09715 0.67 +0.5 2,059,999 + IAI46_09715 0.87 +0.6 2,060,120 + +0.4 2,060,142 - +1.0 2,060,314 - +2.4 2,060,314 - +0.1 2,060,340 + +1.6 2,060,340 + -0.5 2,060,341 - -1.0 2,060,345 - +0.2 2,060,345 - +1.6 2,060,389 + -0.8 2,060,493 + IAI46_09720 0.17 +0.3 2,060,494 - IAI46_09720 0.17 -0.6 2,060,498 + IAI46_09720 0.17 -0.6 2,060,499 - IAI46_09720 0.17 +0.3 2,060,499 - IAI46_09720 0.17 +0.9 2,060,499 - IAI46_09720 0.17 +1.2 2,060,499 - IAI46_09720 0.17 +0.1 2,060,518 + IAI46_09720 0.19 +0.4 2,060,684 + IAI46_09720 0.34 +0.5 2,060,684 + IAI46_09720 0.34 -0.8 2,060,685 - IAI46_09720 0.34 -0.6 2,060,735 - IAI46_09720 0.38 -0.8 2,060,771 - IAI46_09720 0.41 -0.0 2,061,263 + IAI46_09720 0.86 +1.0 2,061,331 + -0.1 2,061,337 + +0.0 2,061,338 - -0.1 2,061,366 - -0.7 2,061,368 + +0.1 2,061,409 + +1.0 2,061,409 + +0.3 2,061,410 - +0.8 2,061,417 - -0.4 2,061,430 + +0.0 2,061,443 - +0.4 2,061,467 - -0.3 2,061,568 + -1.0 2,061,569 - -0.5 2,061,685 - IAI46_09725 0.46 +1.1 2,061,685 - IAI46_09725 0.46 +0.6 2,061,725 - IAI46_09725 0.62 -0.3 2,061,735 + IAI46_09725 0.66 -0.4 2,061,736 - IAI46_09725 0.66 +0.7 2,061,751 + IAI46_09725 0.72 +0.6 2,061,765 - IAI46_09725 0.78 +1.0 2,061,770 + IAI46_09725 0.80 -0.4 2,061,801 + +0.2 2,061,839 + +0.1 2,061,842 + +0.0 2,061,842 + +1.0 2,061,843 - +0.1 2,061,843 - -0.4 2,061,846 + +0.9 2,061,846 + +0.4 2,061,847 - +0.3 2,061,897 + -0.5 2,061,898 - -0.6 2,061,898 - +3.0 2,061,902 + +0.8 2,061,903 - +1.6 2,061,905 - -0.0 2,061,996 + +1.3 2,061,997 - +1.1 2,062,000 + -0.8 2,062,000 + -0.3 2,062,000 + -0.3 2,062,000 + -0.4 2,062,029 + -2.0 2,062,153 + IAI46_09730 0.47 -0.2 2,062,153 + IAI46_09730 0.47 -2.5 2,062,153 + IAI46_09730 0.47 -1.0 2,062,210 + IAI46_09730 0.68 -0.3 2,062,340 - +1.3
Or see this region's nucleotide sequence