Experiment: Nickel (II) chloride 80 uM, anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_08175 and IAI46_08180 are separated by 311 nucleotides IAI46_08180 and IAI46_08185 overlap by 7 nucleotides IAI46_08185 and IAI46_08190 overlap by 19 nucleotides
IAI46_08175: IAI46_08175 - transposase domain-containing protein, at 1,741,671 to 1,742,698
_08175
IAI46_08180: IAI46_08180 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 1,743,010 to 1,743,630
_08180
IAI46_08185: IAI46_08185 - imidazole glycerol phosphate synthase subunit HisF, at 1,743,624 to 1,744,400
_08185
IAI46_08190: IAI46_08190 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,744,382 to 1,745,119
_08190
Position (kb)
1743
1744 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1742.069 kb on + strand, within IAI46_08175 at 1742.069 kb on + strand, within IAI46_08175 at 1742.071 kb on + strand, within IAI46_08175 at 1742.071 kb on + strand, within IAI46_08175 at 1742.071 kb on + strand, within IAI46_08175 at 1742.071 kb on + strand, within IAI46_08175 at 1742.072 kb on - strand, within IAI46_08175 at 1742.072 kb on - strand, within IAI46_08175 at 1742.072 kb on - strand, within IAI46_08175 at 1742.072 kb on - strand, within IAI46_08175 at 1742.072 kb on - strand, within IAI46_08175 at 1742.072 kb on - strand, within IAI46_08175 at 1742.076 kb on - strand, within IAI46_08175 at 1742.237 kb on + strand, within IAI46_08175 at 1742.238 kb on - strand, within IAI46_08175 at 1742.238 kb on - strand, within IAI46_08175 at 1742.277 kb on + strand, within IAI46_08175 at 1742.351 kb on - strand, within IAI46_08175 at 1742.351 kb on - strand, within IAI46_08175 at 1742.408 kb on + strand, within IAI46_08175 at 1742.409 kb on - strand, within IAI46_08175 at 1742.462 kb on + strand, within IAI46_08175 at 1742.463 kb on - strand, within IAI46_08175 at 1742.693 kb on - strand at 1742.717 kb on + strand at 1742.751 kb on + strand at 1742.752 kb on - strand at 1742.755 kb on - strand at 1742.764 kb on - strand at 1742.778 kb on - strand at 1742.901 kb on + strand at 1742.902 kb on - strand at 1742.902 kb on - strand at 1742.910 kb on - strand at 1742.979 kb on - strand at 1742.979 kb on - strand at 1742.979 kb on - strand at 1743.002 kb on + strand at 1743.003 kb on - strand at 1743.011 kb on + strand at 1743.012 kb on - strand at 1743.195 kb on + strand, within IAI46_08180 at 1743.230 kb on + strand, within IAI46_08180 at 1743.327 kb on + strand, within IAI46_08180 at 1743.409 kb on + strand, within IAI46_08180 at 1743.409 kb on + strand, within IAI46_08180 at 1743.409 kb on + strand, within IAI46_08180 at 1743.409 kb on + strand, within IAI46_08180 at 1743.410 kb on - strand, within IAI46_08180 at 1743.463 kb on - strand, within IAI46_08180 at 1743.463 kb on - strand, within IAI46_08180 at 1743.525 kb on - strand, within IAI46_08180 at 1743.839 kb on - strand, within IAI46_08185 at 1743.967 kb on + strand, within IAI46_08185 at 1743.968 kb on - strand, within IAI46_08185 at 1743.968 kb on - strand, within IAI46_08185 at 1744.252 kb on + strand, within IAI46_08185 at 1744.286 kb on - strand, within IAI46_08185 at 1744.350 kb on + strand at 1744.421 kb on - strand at 1744.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, anaerobic remove 1,742,069 + IAI46_08175 0.39 -0.2 1,742,069 + IAI46_08175 0.39 -0.3 1,742,071 + IAI46_08175 0.39 +0.9 1,742,071 + IAI46_08175 0.39 +1.8 1,742,071 + IAI46_08175 0.39 +0.8 1,742,071 + IAI46_08175 0.39 +1.6 1,742,072 - IAI46_08175 0.39 +0.6 1,742,072 - IAI46_08175 0.39 -0.5 1,742,072 - IAI46_08175 0.39 -0.1 1,742,072 - IAI46_08175 0.39 +1.6 1,742,072 - IAI46_08175 0.39 +0.9 1,742,072 - IAI46_08175 0.39 -0.6 1,742,076 - IAI46_08175 0.39 +0.4 1,742,237 + IAI46_08175 0.55 +0.5 1,742,238 - IAI46_08175 0.55 -0.2 1,742,238 - IAI46_08175 0.55 -0.6 1,742,277 + IAI46_08175 0.59 -0.2 1,742,351 - IAI46_08175 0.66 +0.7 1,742,351 - IAI46_08175 0.66 +0.3 1,742,408 + IAI46_08175 0.72 -0.1 1,742,409 - IAI46_08175 0.72 +0.2 1,742,462 + IAI46_08175 0.77 -0.5 1,742,463 - IAI46_08175 0.77 -0.2 1,742,693 - -0.5 1,742,717 + -0.0 1,742,751 + +0.5 1,742,752 - +0.4 1,742,755 - +0.6 1,742,764 - -3.1 1,742,778 - -1.8 1,742,901 + +1.2 1,742,902 - -0.1 1,742,902 - +0.2 1,742,910 - -1.2 1,742,979 - +0.2 1,742,979 - +0.4 1,742,979 - +0.6 1,743,002 + +0.9 1,743,003 - -1.1 1,743,011 + +0.9 1,743,012 - -0.3 1,743,195 + IAI46_08180 0.30 -1.4 1,743,230 + IAI46_08180 0.35 +0.4 1,743,327 + IAI46_08180 0.51 -0.3 1,743,409 + IAI46_08180 0.64 -0.6 1,743,409 + IAI46_08180 0.64 +0.6 1,743,409 + IAI46_08180 0.64 -0.3 1,743,409 + IAI46_08180 0.64 +0.3 1,743,410 - IAI46_08180 0.64 +2.2 1,743,463 - IAI46_08180 0.73 +0.8 1,743,463 - IAI46_08180 0.73 -1.6 1,743,525 - IAI46_08180 0.83 +2.4 1,743,839 - IAI46_08185 0.28 -0.5 1,743,967 + IAI46_08185 0.44 +0.2 1,743,968 - IAI46_08185 0.44 -1.7 1,743,968 - IAI46_08185 0.44 -1.7 1,744,252 + IAI46_08185 0.81 +0.4 1,744,286 - IAI46_08185 0.85 -1.7 1,744,350 + -1.4 1,744,421 - -0.2 1,744,426 + -1.6
Or see this region's nucleotide sequence