Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4539 and EX28DRAFT_4540 are separated by 9 nucleotides EX28DRAFT_4540 and EX28DRAFT_4541 are separated by 41 nucleotides EX28DRAFT_4541 and EX28DRAFT_4542 are separated by 186 nucleotides
EX28DRAFT_4539: EX28DRAFT_4539 - uroporphyrinogen decarboxylase (EC 4.1.1.37), at 22,269 to 23,333
_4539
EX28DRAFT_4540: EX28DRAFT_4540 - Endonuclease V (EC 3.1.21.-), at 23,343 to 24,014
_4540
EX28DRAFT_4541: EX28DRAFT_4541 - Uncharacterized protein conserved in bacteria, at 24,056 to 24,646
_4541
EX28DRAFT_4542: EX28DRAFT_4542 - Bacterial nucleoid DNA-binding protein, at 24,833 to 25,105
_4542
Position (kb) 23
24
25 Strain fitness (log2 ratio) -1
0
1
2 at 23.331 kb on + strand at 23.430 kb on + strand, within EX28DRAFT_4540 at 23.661 kb on - strand, within EX28DRAFT_4540 at 24.021 kb on + strand at 24.152 kb on + strand, within EX28DRAFT_4541 at 24.194 kb on - strand, within EX28DRAFT_4541 at 24.197 kb on + strand, within EX28DRAFT_4541 at 24.424 kb on + strand, within EX28DRAFT_4541 at 24.424 kb on + strand, within EX28DRAFT_4541 at 24.424 kb on + strand, within EX28DRAFT_4541 at 24.424 kb on + strand, within EX28DRAFT_4541 at 24.600 kb on + strand at 24.717 kb on - strand at 24.755 kb on + strand at 24.756 kb on - strand at 24.756 kb on - strand at 24.800 kb on + strand at 24.801 kb on - strand at 24.827 kb on + strand at 24.832 kb on + strand at 24.858 kb on + strand at 24.858 kb on + strand at 24.859 kb on - strand at 24.895 kb on + strand, within EX28DRAFT_4542 at 24.913 kb on + strand, within EX28DRAFT_4542 at 25.006 kb on + strand, within EX28DRAFT_4542
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 23,331 + -0.0 23,430 + EX28DRAFT_4540 0.13 -0.1 23,661 - EX28DRAFT_4540 0.47 +1.2 24,021 + -0.4 24,152 + EX28DRAFT_4541 0.16 -0.2 24,194 - EX28DRAFT_4541 0.23 -1.1 24,197 + EX28DRAFT_4541 0.24 +1.4 24,424 + EX28DRAFT_4541 0.62 -1.3 24,424 + EX28DRAFT_4541 0.62 +2.2 24,424 + EX28DRAFT_4541 0.62 +0.5 24,424 + EX28DRAFT_4541 0.62 +0.3 24,600 + -0.9 24,717 - +0.7 24,755 + +0.4 24,756 - +1.2 24,756 - +0.3 24,800 + +0.1 24,801 - +1.0 24,827 + +1.4 24,832 + -1.5 24,858 + -0.1 24,858 + +0.7 24,859 - +0.3 24,895 + EX28DRAFT_4542 0.23 +0.5 24,913 + EX28DRAFT_4542 0.29 -0.0 25,006 + EX28DRAFT_4542 0.63 -0.7
Or see this region's nucleotide sequence