Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4246 and EX28DRAFT_4247 are separated by 3 nucleotides EX28DRAFT_4247 and EX28DRAFT_4248 are separated by 2 nucleotides EX28DRAFT_4248 and EX28DRAFT_4249 are separated by 113 nucleotides EX28DRAFT_4249 and EX28DRAFT_4250 are separated by 217 nucleotides
EX28DRAFT_4246: EX28DRAFT_4246 - excinuclease ABC, A subunit, at 52,582 to 55,404
_4246
EX28DRAFT_4247: EX28DRAFT_4247 - Uncharacterized protein conserved in bacteria, at 55,408 to 55,761
_4247
EX28DRAFT_4248: EX28DRAFT_4248 - secondary thiamine-phosphate synthase enzyme, at 55,764 to 56,180
_4248
EX28DRAFT_4249: EX28DRAFT_4249 - HAD superfamily (subfamily IIIB) phosphatase, TIGR01672, at 56,294 to 57,007
_4249
EX28DRAFT_4250: EX28DRAFT_4250 - Aspartate/tyrosine/aromatic aminotransferase, at 57,225 to 58,418
_4250
Position (kb) 56
57
58 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 55.402 kb on + strand at 55.464 kb on - strand, within EX28DRAFT_4247 at 55.482 kb on - strand, within EX28DRAFT_4247 at 55.485 kb on - strand, within EX28DRAFT_4247 at 55.485 kb on - strand, within EX28DRAFT_4247 at 55.517 kb on + strand, within EX28DRAFT_4247 at 55.674 kb on + strand, within EX28DRAFT_4247 at 55.745 kb on - strand at 55.776 kb on + strand at 55.776 kb on + strand at 55.777 kb on - strand at 55.793 kb on + strand at 55.907 kb on + strand, within EX28DRAFT_4248 at 55.907 kb on + strand, within EX28DRAFT_4248 at 55.952 kb on - strand, within EX28DRAFT_4248 at 56.154 kb on + strand at 56.154 kb on + strand at 56.213 kb on - strand at 56.265 kb on - strand at 56.295 kb on + strand at 56.298 kb on + strand at 56.298 kb on + strand at 56.375 kb on + strand, within EX28DRAFT_4249 at 56.376 kb on - strand, within EX28DRAFT_4249 at 56.376 kb on - strand, within EX28DRAFT_4249 at 56.444 kb on + strand, within EX28DRAFT_4249 at 56.445 kb on - strand, within EX28DRAFT_4249 at 56.445 kb on - strand, within EX28DRAFT_4249 at 56.445 kb on - strand, within EX28DRAFT_4249 at 56.518 kb on + strand, within EX28DRAFT_4249 at 56.607 kb on + strand, within EX28DRAFT_4249 at 56.607 kb on + strand, within EX28DRAFT_4249 at 56.607 kb on + strand, within EX28DRAFT_4249 at 56.608 kb on - strand, within EX28DRAFT_4249 at 56.641 kb on + strand, within EX28DRAFT_4249 at 56.725 kb on - strand, within EX28DRAFT_4249 at 56.745 kb on + strand, within EX28DRAFT_4249 at 56.746 kb on - strand, within EX28DRAFT_4249 at 56.956 kb on + strand at 56.957 kb on - strand at 56.969 kb on + strand at 56.969 kb on + strand at 56.970 kb on - strand at 57.050 kb on + strand at 57.227 kb on - strand at 57.293 kb on + strand at 57.294 kb on - strand at 57.294 kb on - strand at 57.367 kb on + strand, within EX28DRAFT_4250 at 57.408 kb on - strand, within EX28DRAFT_4250 at 57.484 kb on - strand, within EX28DRAFT_4250 at 57.484 kb on - strand, within EX28DRAFT_4250 at 57.568 kb on + strand, within EX28DRAFT_4250 at 57.775 kb on + strand, within EX28DRAFT_4250 at 57.816 kb on + strand, within EX28DRAFT_4250 at 57.817 kb on - strand, within EX28DRAFT_4250
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 55,402 + +0.5 55,464 - EX28DRAFT_4247 0.16 -0.6 55,482 - EX28DRAFT_4247 0.21 -0.5 55,485 - EX28DRAFT_4247 0.22 +1.1 55,485 - EX28DRAFT_4247 0.22 +0.1 55,517 + EX28DRAFT_4247 0.31 -0.1 55,674 + EX28DRAFT_4247 0.75 +0.3 55,745 - -0.8 55,776 + +0.2 55,776 + -0.2 55,777 - +0.8 55,793 + +0.0 55,907 + EX28DRAFT_4248 0.34 -0.1 55,907 + EX28DRAFT_4248 0.34 +0.3 55,952 - EX28DRAFT_4248 0.45 -0.2 56,154 + -0.0 56,154 + -0.4 56,213 - -0.9 56,265 - +0.8 56,295 + -0.2 56,298 + -0.5 56,298 + +0.8 56,375 + EX28DRAFT_4249 0.11 +0.2 56,376 - EX28DRAFT_4249 0.11 +0.8 56,376 - EX28DRAFT_4249 0.11 +0.7 56,444 + EX28DRAFT_4249 0.21 +0.4 56,445 - EX28DRAFT_4249 0.21 -0.8 56,445 - EX28DRAFT_4249 0.21 +0.7 56,445 - EX28DRAFT_4249 0.21 -0.1 56,518 + EX28DRAFT_4249 0.31 -1.9 56,607 + EX28DRAFT_4249 0.44 -0.3 56,607 + EX28DRAFT_4249 0.44 -1.0 56,607 + EX28DRAFT_4249 0.44 +0.2 56,608 - EX28DRAFT_4249 0.44 +0.3 56,641 + EX28DRAFT_4249 0.49 -0.8 56,725 - EX28DRAFT_4249 0.60 -3.8 56,745 + EX28DRAFT_4249 0.63 -0.0 56,746 - EX28DRAFT_4249 0.63 -0.4 56,956 + +0.1 56,957 - -0.0 56,969 + -0.2 56,969 + +0.5 56,970 - +0.2 57,050 + -3.0 57,227 - +0.5 57,293 + -1.6 57,294 - -1.6 57,294 - -1.5 57,367 + EX28DRAFT_4250 0.12 -1.4 57,408 - EX28DRAFT_4250 0.15 +0.1 57,484 - EX28DRAFT_4250 0.22 -1.4 57,484 - EX28DRAFT_4250 0.22 -0.3 57,568 + EX28DRAFT_4250 0.29 -0.4 57,775 + EX28DRAFT_4250 0.46 +0.0 57,816 + EX28DRAFT_4250 0.49 -0.1 57,817 - EX28DRAFT_4250 0.50 -0.2
Or see this region's nucleotide sequence