Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4101 and EX28DRAFT_4102 are separated by 6 nucleotides EX28DRAFT_4102 and EX28DRAFT_4103 are separated by 10 nucleotides EX28DRAFT_4103 and EX28DRAFT_4104 overlap by 20 nucleotides EX28DRAFT_4104 and EX28DRAFT_4105 overlap by 4 nucleotides EX28DRAFT_4105 and EX28DRAFT_4106 are separated by 68 nucleotides
EX28DRAFT_4101: EX28DRAFT_4101 - Predicted membrane protein, at 75,242 to 75,829
_4101
EX28DRAFT_4102: EX28DRAFT_4102 - Acyl carrier protein, at 75,836 to 76,087
_4102
EX28DRAFT_4103: EX28DRAFT_4103 - Acyl carrier protein, at 76,098 to 76,355
_4103
EX28DRAFT_4104: EX28DRAFT_4104 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 76,336 to 77,151
_4104
EX28DRAFT_4105: EX28DRAFT_4105 - Beta-ketoacyl synthase, N-terminal domain, at 77,148 to 77,870
_4105
EX28DRAFT_4106: EX28DRAFT_4106 - Methylase involved in ubiquinone/menaquinone biosynthesis, at 77,939 to 79,003
_4106
Position (kb) 76
77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 75.597 kb on + strand, within EX28DRAFT_4101 at 75.648 kb on + strand, within EX28DRAFT_4101 at 75.837 kb on + strand at 75.838 kb on - strand at 75.838 kb on - strand at 75.869 kb on + strand, within EX28DRAFT_4102 at 75.870 kb on - strand, within EX28DRAFT_4102 at 76.065 kb on + strand at 76.193 kb on - strand, within EX28DRAFT_4103 at 76.233 kb on - strand, within EX28DRAFT_4103 at 76.331 kb on + strand at 76.342 kb on + strand at 76.359 kb on + strand at 76.402 kb on + strand at 76.402 kb on + strand at 76.403 kb on - strand at 76.403 kb on - strand at 76.403 kb on - strand at 76.457 kb on + strand, within EX28DRAFT_4104 at 76.458 kb on - strand, within EX28DRAFT_4104 at 76.526 kb on + strand, within EX28DRAFT_4104 at 76.847 kb on + strand, within EX28DRAFT_4104 at 76.847 kb on + strand, within EX28DRAFT_4104 at 77.327 kb on - strand, within EX28DRAFT_4105 at 77.445 kb on + strand, within EX28DRAFT_4105 at 77.446 kb on - strand, within EX28DRAFT_4105 at 77.446 kb on - strand, within EX28DRAFT_4105 at 77.480 kb on + strand, within EX28DRAFT_4105 at 77.480 kb on + strand, within EX28DRAFT_4105 at 77.481 kb on - strand, within EX28DRAFT_4105 at 77.523 kb on - strand, within EX28DRAFT_4105 at 77.872 kb on + strand at 77.873 kb on - strand at 77.891 kb on + strand at 77.891 kb on + strand at 77.891 kb on + strand at 77.930 kb on + strand at 77.931 kb on - strand at 77.940 kb on + strand at 77.966 kb on + strand at 77.966 kb on + strand at 77.971 kb on + strand at 77.971 kb on + strand at 77.972 kb on - strand at 77.972 kb on - strand at 78.037 kb on - strand at 78.065 kb on - strand, within EX28DRAFT_4106 at 78.067 kb on + strand, within EX28DRAFT_4106 at 78.067 kb on + strand, within EX28DRAFT_4106 at 78.068 kb on - strand, within EX28DRAFT_4106 at 78.068 kb on - strand, within EX28DRAFT_4106 at 78.079 kb on + strand, within EX28DRAFT_4106 at 78.079 kb on + strand, within EX28DRAFT_4106 at 78.079 kb on + strand, within EX28DRAFT_4106 at 78.080 kb on - strand, within EX28DRAFT_4106 at 78.090 kb on + strand, within EX28DRAFT_4106
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 75,597 + EX28DRAFT_4101 0.60 -0.1 75,648 + EX28DRAFT_4101 0.69 +0.3 75,837 + +0.8 75,838 - +0.6 75,838 - -0.1 75,869 + EX28DRAFT_4102 0.13 +0.6 75,870 - EX28DRAFT_4102 0.13 +1.1 76,065 + +0.9 76,193 - EX28DRAFT_4103 0.37 +0.5 76,233 - EX28DRAFT_4103 0.52 -0.2 76,331 + -0.1 76,342 + +0.6 76,359 + -1.1 76,402 + +0.0 76,402 + -0.1 76,403 - +0.5 76,403 - +1.6 76,403 - -1.0 76,457 + EX28DRAFT_4104 0.15 -0.3 76,458 - EX28DRAFT_4104 0.15 +0.7 76,526 + EX28DRAFT_4104 0.23 +0.8 76,847 + EX28DRAFT_4104 0.63 +0.9 76,847 + EX28DRAFT_4104 0.63 +0.3 77,327 - EX28DRAFT_4105 0.25 +0.6 77,445 + EX28DRAFT_4105 0.41 +0.0 77,446 - EX28DRAFT_4105 0.41 -0.1 77,446 - EX28DRAFT_4105 0.41 -0.2 77,480 + EX28DRAFT_4105 0.46 -0.4 77,480 + EX28DRAFT_4105 0.46 +1.1 77,481 - EX28DRAFT_4105 0.46 +0.7 77,523 - EX28DRAFT_4105 0.52 -0.0 77,872 + +0.3 77,873 - +0.1 77,891 + -0.8 77,891 + +0.4 77,891 + +0.3 77,930 + +0.3 77,931 - -0.1 77,940 + +0.9 77,966 + -0.2 77,966 + +0.6 77,971 + -0.2 77,971 + +0.4 77,972 - -2.9 77,972 - -0.1 78,037 - -0.2 78,065 - EX28DRAFT_4106 0.12 -1.9 78,067 + EX28DRAFT_4106 0.12 -0.9 78,067 + EX28DRAFT_4106 0.12 -0.2 78,068 - EX28DRAFT_4106 0.12 +0.2 78,068 - EX28DRAFT_4106 0.12 -0.6 78,079 + EX28DRAFT_4106 0.13 +1.0 78,079 + EX28DRAFT_4106 0.13 -0.0 78,079 + EX28DRAFT_4106 0.13 -1.0 78,080 - EX28DRAFT_4106 0.13 -0.1 78,090 + EX28DRAFT_4106 0.14 -0.3
Or see this region's nucleotide sequence