Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3845 and EX28DRAFT_3846 are separated by 103 nucleotides EX28DRAFT_3846 and EX28DRAFT_3847 are separated by 90 nucleotides
EX28DRAFT_3845: EX28DRAFT_3845 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 53,741 to 55,126
_3845
EX28DRAFT_3846: EX28DRAFT_3846 - Glutathione S-transferase, at 55,230 to 55,838
_3846
EX28DRAFT_3847: EX28DRAFT_3847 - Multidrug resistance efflux pump, at 55,929 to 57,065
_3847
Position (kb) 55
56 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 54.310 kb on + strand, within EX28DRAFT_3845 at 54.436 kb on + strand, within EX28DRAFT_3845 at 54.441 kb on + strand, within EX28DRAFT_3845 at 54.441 kb on + strand, within EX28DRAFT_3845 at 54.441 kb on + strand, within EX28DRAFT_3845 at 54.442 kb on - strand, within EX28DRAFT_3845 at 54.442 kb on - strand, within EX28DRAFT_3845 at 54.442 kb on - strand, within EX28DRAFT_3845 at 54.442 kb on - strand, within EX28DRAFT_3845 at 54.498 kb on + strand, within EX28DRAFT_3845 at 54.545 kb on - strand, within EX28DRAFT_3845 at 54.771 kb on + strand, within EX28DRAFT_3845 at 54.772 kb on - strand, within EX28DRAFT_3845 at 55.010 kb on + strand at 55.104 kb on + strand at 55.105 kb on - strand at 55.143 kb on + strand at 55.147 kb on + strand at 55.147 kb on + strand at 55.197 kb on - strand at 55.220 kb on - strand at 55.220 kb on - strand at 55.221 kb on + strand at 55.633 kb on + strand, within EX28DRAFT_3846 at 55.633 kb on + strand, within EX28DRAFT_3846 at 55.633 kb on + strand, within EX28DRAFT_3846 at 55.634 kb on - strand, within EX28DRAFT_3846 at 55.634 kb on - strand, within EX28DRAFT_3846 at 55.663 kb on + strand, within EX28DRAFT_3846 at 55.712 kb on - strand, within EX28DRAFT_3846 at 55.739 kb on - strand, within EX28DRAFT_3846 at 55.819 kb on + strand at 55.819 kb on + strand at 55.899 kb on + strand at 55.899 kb on + strand at 55.899 kb on + strand at 55.899 kb on + strand at 55.900 kb on - strand at 55.930 kb on + strand at 55.942 kb on + strand at 55.948 kb on + strand at 55.949 kb on - strand at 55.949 kb on - strand at 55.978 kb on - strand at 55.982 kb on + strand at 56.017 kb on + strand at 56.035 kb on + strand at 56.035 kb on + strand at 56.036 kb on - strand at 56.036 kb on - strand at 56.036 kb on - strand at 56.036 kb on - strand at 56.036 kb on - strand at 56.036 kb on - strand at 56.039 kb on + strand at 56.310 kb on - strand, within EX28DRAFT_3847 at 56.348 kb on - strand, within EX28DRAFT_3847 at 56.380 kb on + strand, within EX28DRAFT_3847 at 56.380 kb on + strand, within EX28DRAFT_3847 at 56.380 kb on + strand, within EX28DRAFT_3847 at 56.381 kb on - strand, within EX28DRAFT_3847 at 56.422 kb on + strand, within EX28DRAFT_3847 at 56.483 kb on + strand, within EX28DRAFT_3847 at 56.483 kb on + strand, within EX28DRAFT_3847 at 56.484 kb on - strand, within EX28DRAFT_3847 at 56.484 kb on - strand, within EX28DRAFT_3847 at 56.533 kb on - strand, within EX28DRAFT_3847 at 56.554 kb on + strand, within EX28DRAFT_3847 at 56.554 kb on + strand, within EX28DRAFT_3847 at 56.555 kb on - strand, within EX28DRAFT_3847 at 56.599 kb on - strand, within EX28DRAFT_3847 at 56.632 kb on + strand, within EX28DRAFT_3847 at 56.633 kb on - strand, within EX28DRAFT_3847 at 56.640 kb on - strand, within EX28DRAFT_3847 at 56.641 kb on + strand, within EX28DRAFT_3847 at 56.642 kb on - strand, within EX28DRAFT_3847 at 56.793 kb on + strand, within EX28DRAFT_3847 at 56.814 kb on - strand, within EX28DRAFT_3847 at 56.828 kb on + strand, within EX28DRAFT_3847
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 54,310 + EX28DRAFT_3845 0.41 +0.3 54,436 + EX28DRAFT_3845 0.50 -0.1 54,441 + EX28DRAFT_3845 0.51 -0.7 54,441 + EX28DRAFT_3845 0.51 -0.3 54,441 + EX28DRAFT_3845 0.51 -1.3 54,442 - EX28DRAFT_3845 0.51 +0.3 54,442 - EX28DRAFT_3845 0.51 +0.7 54,442 - EX28DRAFT_3845 0.51 +0.8 54,442 - EX28DRAFT_3845 0.51 -0.3 54,498 + EX28DRAFT_3845 0.55 +0.2 54,545 - EX28DRAFT_3845 0.58 +0.3 54,771 + EX28DRAFT_3845 0.74 +0.2 54,772 - EX28DRAFT_3845 0.74 -0.3 55,010 + +0.2 55,104 + -0.2 55,105 - +2.5 55,143 + +0.3 55,147 + +0.4 55,147 + +0.9 55,197 - +0.7 55,220 - -0.2 55,220 - -0.0 55,221 + +0.2 55,633 + EX28DRAFT_3846 0.66 +0.2 55,633 + EX28DRAFT_3846 0.66 -0.5 55,633 + EX28DRAFT_3846 0.66 +0.3 55,634 - EX28DRAFT_3846 0.66 +0.5 55,634 - EX28DRAFT_3846 0.66 +0.3 55,663 + EX28DRAFT_3846 0.71 +0.7 55,712 - EX28DRAFT_3846 0.79 +0.0 55,739 - EX28DRAFT_3846 0.84 +0.6 55,819 + -0.5 55,819 + +0.9 55,899 + +1.5 55,899 + -2.3 55,899 + +0.3 55,899 + +1.3 55,900 - -0.2 55,930 + +1.4 55,942 + -0.3 55,948 + +0.7 55,949 - -0.8 55,949 - +0.1 55,978 - +0.6 55,982 + +2.0 56,017 + +0.0 56,035 + +1.6 56,035 + +0.3 56,036 - -0.5 56,036 - -0.3 56,036 - +0.7 56,036 - +0.1 56,036 - -0.2 56,036 - +0.3 56,039 + +0.2 56,310 - EX28DRAFT_3847 0.34 -0.6 56,348 - EX28DRAFT_3847 0.37 -1.7 56,380 + EX28DRAFT_3847 0.40 -0.1 56,380 + EX28DRAFT_3847 0.40 +0.4 56,380 + EX28DRAFT_3847 0.40 +0.5 56,381 - EX28DRAFT_3847 0.40 -1.0 56,422 + EX28DRAFT_3847 0.43 +0.2 56,483 + EX28DRAFT_3847 0.49 +0.4 56,483 + EX28DRAFT_3847 0.49 -3.3 56,484 - EX28DRAFT_3847 0.49 -0.3 56,484 - EX28DRAFT_3847 0.49 +0.8 56,533 - EX28DRAFT_3847 0.53 +0.5 56,554 + EX28DRAFT_3847 0.55 +0.2 56,554 + EX28DRAFT_3847 0.55 +0.5 56,555 - EX28DRAFT_3847 0.55 -0.5 56,599 - EX28DRAFT_3847 0.59 +0.1 56,632 + EX28DRAFT_3847 0.62 +0.0 56,633 - EX28DRAFT_3847 0.62 +0.7 56,640 - EX28DRAFT_3847 0.63 -0.4 56,641 + EX28DRAFT_3847 0.63 +0.5 56,642 - EX28DRAFT_3847 0.63 +0.4 56,793 + EX28DRAFT_3847 0.76 +0.3 56,814 - EX28DRAFT_3847 0.78 -2.2 56,828 + EX28DRAFT_3847 0.79 -0.4
Or see this region's nucleotide sequence