Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3744 and EX28DRAFT_3745 are separated by 79 nucleotides EX28DRAFT_3745 and EX28DRAFT_3746 are separated by 71 nucleotides EX28DRAFT_3746 and EX28DRAFT_3747 are separated by 27 nucleotides EX28DRAFT_3747 and EX28DRAFT_3748 overlap by 8 nucleotides
EX28DRAFT_3744: EX28DRAFT_3744 - Chloride channel protein EriC, at 423,788 to 425,188
_3744
EX28DRAFT_3745: EX28DRAFT_3745 - Iron-sulfur cluster assembly accessory protein, at 425,268 to 425,615
_3745
EX28DRAFT_3746: EX28DRAFT_3746 - Predicted membrane protein, at 425,687 to 426,310
_3746
EX28DRAFT_3747: EX28DRAFT_3747 - ABC-type Fe3+-hydroxamate transport system, periplasmic component, at 426,338 to 427,138
_3747
EX28DRAFT_3748: EX28DRAFT_3748 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 427,131 to 427,829
_3748
Position (kb) 425
426
427 Strain fitness (log2 ratio) -2
-1
0
1
2 at 424.792 kb on + strand, within EX28DRAFT_3744 at 424.793 kb on - strand, within EX28DRAFT_3744 at 424.793 kb on - strand, within EX28DRAFT_3744 at 424.799 kb on - strand, within EX28DRAFT_3744 at 425.042 kb on + strand, within EX28DRAFT_3744 at 425.042 kb on + strand, within EX28DRAFT_3744 at 425.043 kb on - strand, within EX28DRAFT_3744 at 425.043 kb on - strand, within EX28DRAFT_3744 at 425.065 kb on - strand at 425.068 kb on + strand at 425.069 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.091 kb on + strand at 425.092 kb on - strand at 425.120 kb on + strand at 425.140 kb on + strand at 425.141 kb on - strand at 425.183 kb on - strand at 425.206 kb on + strand at 425.614 kb on - strand at 425.632 kb on - strand at 425.753 kb on + strand, within EX28DRAFT_3746 at 425.754 kb on - strand, within EX28DRAFT_3746 at 425.815 kb on - strand, within EX28DRAFT_3746 at 425.815 kb on - strand, within EX28DRAFT_3746 at 425.913 kb on - strand, within EX28DRAFT_3746 at 426.334 kb on + strand at 426.334 kb on + strand at 426.334 kb on + strand at 426.335 kb on - strand at 426.335 kb on - strand at 426.335 kb on - strand at 426.335 kb on - strand at 426.335 kb on - strand at 426.388 kb on + strand at 426.389 kb on - strand at 426.438 kb on - strand, within EX28DRAFT_3747 at 426.444 kb on - strand, within EX28DRAFT_3747 at 426.676 kb on + strand, within EX28DRAFT_3747 at 426.685 kb on + strand, within EX28DRAFT_3747 at 426.711 kb on + strand, within EX28DRAFT_3747 at 426.763 kb on + strand, within EX28DRAFT_3747 at 426.766 kb on - strand, within EX28DRAFT_3747 at 426.834 kb on - strand, within EX28DRAFT_3747 at 426.994 kb on + strand, within EX28DRAFT_3747 at 426.995 kb on - strand, within EX28DRAFT_3747 at 427.039 kb on + strand, within EX28DRAFT_3747 at 427.040 kb on - strand, within EX28DRAFT_3747 at 427.057 kb on + strand, within EX28DRAFT_3747 at 427.078 kb on - strand at 427.078 kb on - strand at 427.126 kb on + strand at 427.127 kb on - strand at 427.133 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 424,792 + EX28DRAFT_3744 0.72 +0.3 424,793 - EX28DRAFT_3744 0.72 +2.0 424,793 - EX28DRAFT_3744 0.72 +0.2 424,799 - EX28DRAFT_3744 0.72 +0.1 425,042 + EX28DRAFT_3744 0.90 -0.5 425,042 + EX28DRAFT_3744 0.90 +0.1 425,043 - EX28DRAFT_3744 0.90 -1.1 425,043 - EX28DRAFT_3744 0.90 -1.0 425,065 - -0.7 425,068 + -1.7 425,069 - +1.1 425,089 - -0.1 425,089 - -1.0 425,089 - +0.2 425,089 - +0.8 425,089 - -0.9 425,089 - +0.1 425,089 - +0.8 425,089 - +0.0 425,091 + -0.7 425,092 - +0.3 425,120 + +0.4 425,140 + +0.1 425,141 - +0.2 425,183 - +0.9 425,206 + +0.2 425,614 - +0.2 425,632 - +0.5 425,753 + EX28DRAFT_3746 0.11 +1.5 425,754 - EX28DRAFT_3746 0.11 -1.2 425,815 - EX28DRAFT_3746 0.21 +1.2 425,815 - EX28DRAFT_3746 0.21 +0.8 425,913 - EX28DRAFT_3746 0.36 -0.3 426,334 + -0.6 426,334 + -1.8 426,334 + -0.9 426,335 - +0.4 426,335 - +0.7 426,335 - -0.1 426,335 - -0.1 426,335 - -0.6 426,388 + +0.2 426,389 - -1.4 426,438 - EX28DRAFT_3747 0.12 -0.1 426,444 - EX28DRAFT_3747 0.13 +0.7 426,676 + EX28DRAFT_3747 0.42 +1.1 426,685 + EX28DRAFT_3747 0.43 -0.1 426,711 + EX28DRAFT_3747 0.47 -2.4 426,763 + EX28DRAFT_3747 0.53 +1.2 426,766 - EX28DRAFT_3747 0.53 -2.4 426,834 - EX28DRAFT_3747 0.62 -0.5 426,994 + EX28DRAFT_3747 0.82 +0.8 426,995 - EX28DRAFT_3747 0.82 +0.5 427,039 + EX28DRAFT_3747 0.88 -2.7 427,040 - EX28DRAFT_3747 0.88 -1.8 427,057 + EX28DRAFT_3747 0.90 -0.5 427,078 - +0.5 427,078 - -0.1 427,126 + -0.3 427,127 - +0.3 427,133 - +0.7
Or see this region's nucleotide sequence