Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3164 and EX28DRAFT_3165 are separated by 142 nucleotides EX28DRAFT_3165 and EX28DRAFT_3166 are separated by 31 nucleotides EX28DRAFT_3166 and EX28DRAFT_3167 are separated by 1 nucleotides EX28DRAFT_3167 and EX28DRAFT_3168 are separated by 2 nucleotides EX28DRAFT_3168 and EX28DRAFT_3169 are separated by 252 nucleotides
EX28DRAFT_3164: EX28DRAFT_3164 - SecD export protein N-terminal TM region, at 467,838 to 468,221
_3164
EX28DRAFT_3165: EX28DRAFT_3165 - Protein of unknown function (DUF1090), at 468,364 to 468,732
_3165
EX28DRAFT_3166: EX28DRAFT_3166 - Uncharacterized conserved protein, at 468,764 to 469,069
_3166
EX28DRAFT_3167: EX28DRAFT_3167 - Predicted membrane protein, at 469,071 to 469,463
_3167
EX28DRAFT_3168: EX28DRAFT_3168 - YqjK-like protein, at 469,466 to 469,762
_3168
EX28DRAFT_3169: EX28DRAFT_3169 - Predicted membrane protein, at 470,015 to 470,407
_3169
Position (kb) 469
470 Strain fitness (log2 ratio) -2
-1
0
1
2 at 468.138 kb on + strand, within EX28DRAFT_3164 at 468.139 kb on - strand, within EX28DRAFT_3164 at 468.140 kb on + strand, within EX28DRAFT_3164 at 468.140 kb on + strand, within EX28DRAFT_3164 at 468.141 kb on - strand, within EX28DRAFT_3164 at 468.141 kb on - strand, within EX28DRAFT_3164 at 468.220 kb on - strand at 468.269 kb on + strand at 468.269 kb on + strand at 468.270 kb on - strand at 468.273 kb on + strand at 468.273 kb on + strand at 468.274 kb on - strand at 468.274 kb on - strand at 468.276 kb on + strand at 468.276 kb on + strand at 468.277 kb on - strand at 468.282 kb on + strand at 468.282 kb on + strand at 468.282 kb on + strand at 468.282 kb on + strand at 468.283 kb on - strand at 468.283 kb on - strand at 468.404 kb on + strand, within EX28DRAFT_3165 at 468.472 kb on + strand, within EX28DRAFT_3165 at 468.486 kb on + strand, within EX28DRAFT_3165 at 468.513 kb on + strand, within EX28DRAFT_3165 at 468.986 kb on + strand, within EX28DRAFT_3166 at 469.014 kb on + strand, within EX28DRAFT_3166 at 469.103 kb on + strand at 469.104 kb on - strand at 469.131 kb on - strand, within EX28DRAFT_3167 at 469.131 kb on - strand, within EX28DRAFT_3167 at 469.502 kb on + strand, within EX28DRAFT_3168 at 469.502 kb on + strand, within EX28DRAFT_3168 at 469.503 kb on - strand, within EX28DRAFT_3168 at 469.597 kb on + strand, within EX28DRAFT_3168 at 469.672 kb on + strand, within EX28DRAFT_3168 at 470.123 kb on + strand, within EX28DRAFT_3169 at 470.123 kb on + strand, within EX28DRAFT_3169 at 470.123 kb on + strand, within EX28DRAFT_3169 at 470.124 kb on - strand, within EX28DRAFT_3169 at 470.124 kb on - strand, within EX28DRAFT_3169 at 470.124 kb on - strand, within EX28DRAFT_3169 at 470.124 kb on - strand, within EX28DRAFT_3169 at 470.124 kb on - strand, within EX28DRAFT_3169 at 470.125 kb on + strand, within EX28DRAFT_3169 at 470.125 kb on + strand, within EX28DRAFT_3169 at 470.125 kb on + strand, within EX28DRAFT_3169 at 470.396 kb on - strand at 470.396 kb on - strand at 470.405 kb on + strand at 470.406 kb on - strand at 470.414 kb on + strand at 470.415 kb on - strand at 470.442 kb on + strand at 470.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 468,138 + EX28DRAFT_3164 0.78 -0.4 468,139 - EX28DRAFT_3164 0.78 -1.3 468,140 + EX28DRAFT_3164 0.79 -0.7 468,140 + EX28DRAFT_3164 0.79 +0.0 468,141 - EX28DRAFT_3164 0.79 -0.4 468,141 - EX28DRAFT_3164 0.79 +0.0 468,220 - -0.3 468,269 + -0.8 468,269 + +0.3 468,270 - +0.1 468,273 + -0.0 468,273 + -0.7 468,274 - -0.7 468,274 - -1.7 468,276 + +0.1 468,276 + -0.1 468,277 - -0.9 468,282 + +1.4 468,282 + +0.7 468,282 + +0.9 468,282 + -0.2 468,283 - +0.2 468,283 - -0.1 468,404 + EX28DRAFT_3165 0.11 -0.2 468,472 + EX28DRAFT_3165 0.29 +0.1 468,486 + EX28DRAFT_3165 0.33 -0.1 468,513 + EX28DRAFT_3165 0.40 -1.1 468,986 + EX28DRAFT_3166 0.73 -1.0 469,014 + EX28DRAFT_3166 0.82 -0.3 469,103 + +0.3 469,104 - -0.4 469,131 - EX28DRAFT_3167 0.15 -0.0 469,131 - EX28DRAFT_3167 0.15 +0.9 469,502 + EX28DRAFT_3168 0.12 +0.2 469,502 + EX28DRAFT_3168 0.12 -0.2 469,503 - EX28DRAFT_3168 0.12 -0.2 469,597 + EX28DRAFT_3168 0.44 -0.2 469,672 + EX28DRAFT_3168 0.69 +2.5 470,123 + EX28DRAFT_3169 0.27 +0.3 470,123 + EX28DRAFT_3169 0.27 +0.2 470,123 + EX28DRAFT_3169 0.27 -0.6 470,124 - EX28DRAFT_3169 0.28 -1.3 470,124 - EX28DRAFT_3169 0.28 -2.1 470,124 - EX28DRAFT_3169 0.28 -2.7 470,124 - EX28DRAFT_3169 0.28 -0.1 470,124 - EX28DRAFT_3169 0.28 +0.5 470,125 + EX28DRAFT_3169 0.28 -0.3 470,125 + EX28DRAFT_3169 0.28 +0.8 470,125 + EX28DRAFT_3169 0.28 +0.2 470,396 - -1.2 470,396 - +0.1 470,405 + -2.0 470,406 - -1.4 470,414 + +0.2 470,415 - -0.4 470,442 + -0.9 470,443 - +0.7
Or see this region's nucleotide sequence