Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3118 and EX28DRAFT_3119 are separated by 5 nucleotides EX28DRAFT_3119 and EX28DRAFT_3120 overlap by 4 nucleotides EX28DRAFT_3120 and EX28DRAFT_3121 are separated by 130 nucleotides EX28DRAFT_3121 and EX28DRAFT_3122 overlap by 38 nucleotides EX28DRAFT_3122 and EX28DRAFT_3123 overlap by 26 nucleotides
EX28DRAFT_3118: EX28DRAFT_3118 - Phage-related baseplate assembly protein, at 424,914 to 425,822
_3118
EX28DRAFT_3119: EX28DRAFT_3119 - Phage baseplate assembly protein W, at 425,828 to 426,178
_3119
EX28DRAFT_3120: EX28DRAFT_3120 - phage baseplate assembly protein V, at 426,175 to 426,798
_3120
EX28DRAFT_3121: EX28DRAFT_3121 - P2 phage tail completion protein R (GpR), at 426,929 to 427,396
_3121
EX28DRAFT_3122: EX28DRAFT_3122 - hypothetical protein, at 427,359 to 427,517
_3122
EX28DRAFT_3123: EX28DRAFT_3123 - phage lysis regulatory protein, LysB family, at 427,492 to 427,935
_3123
Position (kb) 426
427 Strain fitness (log2 ratio) -1
0
1 at 425.185 kb on - strand, within EX28DRAFT_3118 at 425.243 kb on + strand, within EX28DRAFT_3118 at 425.243 kb on + strand, within EX28DRAFT_3118 at 425.244 kb on - strand, within EX28DRAFT_3118 at 425.392 kb on + strand, within EX28DRAFT_3118 at 425.495 kb on + strand, within EX28DRAFT_3118 at 425.496 kb on - strand, within EX28DRAFT_3118 at 425.535 kb on - strand, within EX28DRAFT_3118 at 425.817 kb on - strand at 425.848 kb on + strand at 425.848 kb on + strand at 425.895 kb on + strand, within EX28DRAFT_3119 at 425.895 kb on + strand, within EX28DRAFT_3119 at 425.896 kb on - strand, within EX28DRAFT_3119 at 425.896 kb on - strand, within EX28DRAFT_3119 at 425.896 kb on - strand, within EX28DRAFT_3119 at 425.896 kb on - strand, within EX28DRAFT_3119 at 425.896 kb on - strand, within EX28DRAFT_3119 at 426.189 kb on + strand at 426.324 kb on + strand, within EX28DRAFT_3120 at 426.324 kb on + strand, within EX28DRAFT_3120 at 426.324 kb on + strand, within EX28DRAFT_3120 at 426.325 kb on - strand, within EX28DRAFT_3120 at 426.328 kb on + strand, within EX28DRAFT_3120 at 426.329 kb on - strand, within EX28DRAFT_3120 at 426.357 kb on + strand, within EX28DRAFT_3120 at 426.444 kb on + strand, within EX28DRAFT_3120 at 426.810 kb on + strand at 426.810 kb on + strand at 426.811 kb on - strand at 426.877 kb on + strand at 426.878 kb on - strand at 426.967 kb on - strand at 427.017 kb on + strand, within EX28DRAFT_3121 at 427.018 kb on - strand, within EX28DRAFT_3121 at 427.157 kb on - strand, within EX28DRAFT_3121 at 427.494 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 425,185 - EX28DRAFT_3118 0.30 +0.2 425,243 + EX28DRAFT_3118 0.36 +0.2 425,243 + EX28DRAFT_3118 0.36 -0.1 425,244 - EX28DRAFT_3118 0.36 +1.0 425,392 + EX28DRAFT_3118 0.53 -1.7 425,495 + EX28DRAFT_3118 0.64 +0.4 425,496 - EX28DRAFT_3118 0.64 -0.5 425,535 - EX28DRAFT_3118 0.68 +0.8 425,817 - +0.6 425,848 + +0.2 425,848 + +0.2 425,895 + EX28DRAFT_3119 0.19 +0.7 425,895 + EX28DRAFT_3119 0.19 +0.3 425,896 - EX28DRAFT_3119 0.19 +0.7 425,896 - EX28DRAFT_3119 0.19 +0.3 425,896 - EX28DRAFT_3119 0.19 -0.1 425,896 - EX28DRAFT_3119 0.19 +0.7 425,896 - EX28DRAFT_3119 0.19 +0.5 426,189 + +0.4 426,324 + EX28DRAFT_3120 0.24 +0.3 426,324 + EX28DRAFT_3120 0.24 -1.1 426,324 + EX28DRAFT_3120 0.24 -0.9 426,325 - EX28DRAFT_3120 0.24 -0.3 426,328 + EX28DRAFT_3120 0.25 +0.3 426,329 - EX28DRAFT_3120 0.25 -1.7 426,357 + EX28DRAFT_3120 0.29 +0.4 426,444 + EX28DRAFT_3120 0.43 -1.7 426,810 + -1.3 426,810 + +0.2 426,811 - -0.0 426,877 + -1.5 426,878 - -0.4 426,967 - -0.5 427,017 + EX28DRAFT_3121 0.19 +0.1 427,018 - EX28DRAFT_3121 0.19 +0.0 427,157 - EX28DRAFT_3121 0.49 -0.9 427,494 - -0.1
Or see this region's nucleotide sequence