Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3032 and EX28DRAFT_3033 are separated by 141 nucleotides EX28DRAFT_3033 and EX28DRAFT_3034 are separated by 10 nucleotides EX28DRAFT_3034 and EX28DRAFT_3035 are separated by 179 nucleotides EX28DRAFT_3035 and EX28DRAFT_3036 are separated by 52 nucleotides EX28DRAFT_3036 and EX28DRAFT_3037 are separated by 155 nucleotides
EX28DRAFT_3032: EX28DRAFT_3032 - Sugar phosphate isomerases/epimerases, at 343,274 to 344,329
_3032
EX28DRAFT_3033: EX28DRAFT_3033 - transcriptional regulator, TetR family, at 344,471 to 345,061
_3033
EX28DRAFT_3034: EX28DRAFT_3034 - hypothetical protein, at 345,072 to 345,350
_3034
EX28DRAFT_3035: EX28DRAFT_3035 - hypothetical protein, at 345,530 to 345,979
_3035
EX28DRAFT_3036: EX28DRAFT_3036 - RNA, at 346,032 to 346,118
_3036
EX28DRAFT_3037: EX28DRAFT_3037 - hypothetical protein, at 346,274 to 346,528
_3037
Position (kb) 345
346 Strain fitness (log2 ratio) -2
-1
0
1
2 at 344.131 kb on + strand, within EX28DRAFT_3032 at 344.263 kb on + strand at 344.332 kb on + strand at 344.332 kb on + strand at 344.332 kb on + strand at 344.333 kb on - strand at 344.333 kb on - strand at 344.334 kb on + strand at 344.334 kb on + strand at 344.335 kb on - strand at 344.335 kb on - strand at 344.371 kb on - strand at 344.397 kb on + strand at 344.426 kb on - strand at 344.426 kb on - strand at 344.546 kb on + strand, within EX28DRAFT_3033 at 344.547 kb on - strand, within EX28DRAFT_3033 at 344.607 kb on - strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.790 kb on + strand, within EX28DRAFT_3033 at 344.951 kb on + strand, within EX28DRAFT_3033 at 344.951 kb on + strand, within EX28DRAFT_3033 at 345.175 kb on + strand, within EX28DRAFT_3034 at 345.242 kb on + strand, within EX28DRAFT_3034 at 345.243 kb on - strand, within EX28DRAFT_3034 at 345.247 kb on - strand, within EX28DRAFT_3034 at 345.247 kb on - strand, within EX28DRAFT_3034 at 345.251 kb on + strand, within EX28DRAFT_3034 at 345.251 kb on + strand, within EX28DRAFT_3034 at 345.251 kb on + strand, within EX28DRAFT_3034 at 345.252 kb on - strand, within EX28DRAFT_3034 at 345.306 kb on - strand, within EX28DRAFT_3034 at 345.331 kb on - strand at 345.331 kb on - strand at 345.338 kb on - strand at 345.338 kb on - strand at 345.384 kb on - strand at 345.409 kb on + strand at 345.431 kb on - strand at 345.451 kb on + strand at 345.452 kb on - strand at 345.453 kb on + strand at 345.477 kb on + strand at 345.532 kb on - strand at 345.532 kb on - strand at 345.595 kb on - strand, within EX28DRAFT_3035 at 345.690 kb on + strand, within EX28DRAFT_3035 at 345.691 kb on - strand, within EX28DRAFT_3035 at 345.879 kb on + strand, within EX28DRAFT_3035 at 345.886 kb on - strand, within EX28DRAFT_3035 at 346.061 kb on + strand, within EX28DRAFT_3036 at 346.062 kb on - strand, within EX28DRAFT_3036 at 346.090 kb on + strand, within EX28DRAFT_3036 at 346.139 kb on + strand at 346.139 kb on + strand at 346.140 kb on - strand at 346.140 kb on - strand at 346.140 kb on - strand at 346.140 kb on - strand at 346.140 kb on - strand at 346.216 kb on + strand at 346.279 kb on - strand at 346.279 kb on - strand at 346.282 kb on + strand at 346.282 kb on + strand at 346.282 kb on + strand at 346.282 kb on + strand at 346.283 kb on - strand at 346.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 344,131 + EX28DRAFT_3032 0.81 +0.1 344,263 + +0.5 344,332 + -0.2 344,332 + +0.3 344,332 + -0.2 344,333 - -1.0 344,333 - +0.3 344,334 + +0.4 344,334 + +0.1 344,335 - +0.1 344,335 - +0.4 344,371 - -0.8 344,397 + +0.7 344,426 - +1.2 344,426 - +0.1 344,546 + EX28DRAFT_3033 0.13 +1.6 344,547 - EX28DRAFT_3033 0.13 -0.1 344,607 - EX28DRAFT_3033 0.23 -0.1 344,785 + EX28DRAFT_3033 0.53 -0.4 344,785 + EX28DRAFT_3033 0.53 +0.1 344,785 + EX28DRAFT_3033 0.53 -1.0 344,785 + EX28DRAFT_3033 0.53 -0.1 344,785 + EX28DRAFT_3033 0.53 +1.4 344,790 + EX28DRAFT_3033 0.54 -0.7 344,951 + EX28DRAFT_3033 0.81 +1.3 344,951 + EX28DRAFT_3033 0.81 -0.1 345,175 + EX28DRAFT_3034 0.37 +0.9 345,242 + EX28DRAFT_3034 0.61 +0.6 345,243 - EX28DRAFT_3034 0.61 -0.1 345,247 - EX28DRAFT_3034 0.63 +1.1 345,247 - EX28DRAFT_3034 0.63 +1.1 345,251 + EX28DRAFT_3034 0.64 +0.1 345,251 + EX28DRAFT_3034 0.64 -0.5 345,251 + EX28DRAFT_3034 0.64 -1.8 345,252 - EX28DRAFT_3034 0.65 +1.0 345,306 - EX28DRAFT_3034 0.84 +0.6 345,331 - +0.6 345,331 - +2.2 345,338 - +0.7 345,338 - +0.3 345,384 - -1.6 345,409 + +0.9 345,431 - +1.0 345,451 + +0.4 345,452 - +2.1 345,453 + -0.4 345,477 + -0.8 345,532 - +0.2 345,532 - +1.0 345,595 - EX28DRAFT_3035 0.14 +0.7 345,690 + EX28DRAFT_3035 0.36 -0.4 345,691 - EX28DRAFT_3035 0.36 +0.1 345,879 + EX28DRAFT_3035 0.78 +0.0 345,886 - EX28DRAFT_3035 0.79 +0.8 346,061 + EX28DRAFT_3036 0.33 +0.5 346,062 - EX28DRAFT_3036 0.34 +0.3 346,090 + EX28DRAFT_3036 0.67 -1.2 346,139 + +0.4 346,139 + -0.4 346,140 - +0.3 346,140 - +0.6 346,140 - -0.4 346,140 - +1.0 346,140 - +1.0 346,216 + +0.3 346,279 - -0.3 346,279 - +0.3 346,282 + +0.7 346,282 + -0.3 346,282 + -1.1 346,282 + -0.1 346,283 - -0.8 346,283 - -0.1
Or see this region's nucleotide sequence