Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2774 and EX28DRAFT_2775 are separated by 83 nucleotides EX28DRAFT_2775 and EX28DRAFT_2776 overlap by 4 nucleotides EX28DRAFT_2776 and EX28DRAFT_2777 are separated by 117 nucleotides EX28DRAFT_2777 and EX28DRAFT_2778 are separated by 1 nucleotides EX28DRAFT_2778 and EX28DRAFT_2779 are separated by 29 nucleotides EX28DRAFT_2779 and EX28DRAFT_2780 are separated by 29 nucleotides EX28DRAFT_2780 and EX28DRAFT_2781 are separated by 4 nucleotides EX28DRAFT_2781 and EX28DRAFT_2782 are separated by 342 nucleotides
EX28DRAFT_2774: EX28DRAFT_2774 - glutamate-cysteine ligase (EC 6.3.2.2), at 81,896 to 83,440
_2774
EX28DRAFT_2775: EX28DRAFT_2775 - Predicted membrane protein, at 83,524 to 83,952
_2775
EX28DRAFT_2776: EX28DRAFT_2776 - haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED/beta-phosphoglucomutase family hydrolase, at 83,949 to 84,515
_2776
EX28DRAFT_2777: EX28DRAFT_2777 - RNA, at 84,633 to 84,764
_2777
EX28DRAFT_2778: EX28DRAFT_2778 - tRNA, at 84,766 to 84,842
_2778
EX28DRAFT_2779: EX28DRAFT_2779 - tRNA, at 84,872 to 84,948
_2779
EX28DRAFT_2780: EX28DRAFT_2780 - tRNA, at 84,978 to 85,054
_2780
EX28DRAFT_2781: EX28DRAFT_2781 - tRNA, at 85,059 to 85,151
_2781
EX28DRAFT_2782: EX28DRAFT_2782 - carbon storage regulator, CsrA, at 85,494 to 85,679
_2782
Position (kb) 83
84
85 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 82.989 kb on + strand, within EX28DRAFT_2774 at 83.121 kb on + strand, within EX28DRAFT_2774 at 83.121 kb on + strand, within EX28DRAFT_2774 at 83.147 kb on - strand, within EX28DRAFT_2774 at 83.206 kb on + strand, within EX28DRAFT_2774 at 83.216 kb on - strand, within EX28DRAFT_2774 at 83.279 kb on - strand, within EX28DRAFT_2774 at 83.329 kb on + strand at 83.426 kb on + strand at 83.463 kb on + strand at 83.464 kb on - strand at 83.464 kb on - strand at 83.469 kb on + strand at 83.470 kb on - strand at 83.479 kb on - strand at 83.525 kb on + strand at 83.525 kb on + strand at 83.541 kb on + strand at 83.541 kb on + strand at 83.542 kb on - strand at 83.549 kb on - strand at 83.549 kb on - strand at 83.653 kb on + strand, within EX28DRAFT_2775 at 83.654 kb on - strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.666 kb on - strand, within EX28DRAFT_2775 at 83.681 kb on - strand, within EX28DRAFT_2775 at 83.683 kb on + strand, within EX28DRAFT_2775 at 83.776 kb on - strand, within EX28DRAFT_2775 at 83.802 kb on + strand, within EX28DRAFT_2775 at 83.815 kb on + strand, within EX28DRAFT_2775 at 83.818 kb on + strand, within EX28DRAFT_2775 at 83.821 kb on + strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 84.228 kb on + strand, within EX28DRAFT_2776 at 84.229 kb on - strand, within EX28DRAFT_2776 at 84.229 kb on - strand, within EX28DRAFT_2776 at 84.233 kb on + strand, within EX28DRAFT_2776 at 84.503 kb on - strand at 84.511 kb on + strand at 84.511 kb on + strand at 84.512 kb on - strand at 84.512 kb on - strand at 84.546 kb on + strand at 84.556 kb on + strand at 84.557 kb on - strand at 84.718 kb on + strand, within EX28DRAFT_2777 at 84.753 kb on - strand at 84.765 kb on - strand at 84.765 kb on - strand at 84.860 kb on - strand at 84.868 kb on + strand at 84.869 kb on - strand at 85.374 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 82,989 + EX28DRAFT_2774 0.71 +1.4 83,121 + EX28DRAFT_2774 0.79 -3.2 83,121 + EX28DRAFT_2774 0.79 -0.2 83,147 - EX28DRAFT_2774 0.81 +1.8 83,206 + EX28DRAFT_2774 0.85 -0.2 83,216 - EX28DRAFT_2774 0.85 +0.4 83,279 - EX28DRAFT_2774 0.90 -0.2 83,329 + -0.4 83,426 + +0.7 83,463 + -0.9 83,464 - +0.5 83,464 - +0.3 83,469 + -0.5 83,470 - +0.8 83,479 - -0.4 83,525 + -0.1 83,525 + +0.8 83,541 + +0.6 83,541 + -0.3 83,542 - +0.1 83,549 - +0.3 83,549 - -0.1 83,653 + EX28DRAFT_2775 0.30 +0.0 83,654 - EX28DRAFT_2775 0.30 +0.5 83,665 + EX28DRAFT_2775 0.33 +0.4 83,665 + EX28DRAFT_2775 0.33 -0.4 83,665 + EX28DRAFT_2775 0.33 +1.1 83,666 - EX28DRAFT_2775 0.33 -0.0 83,681 - EX28DRAFT_2775 0.37 -0.4 83,683 + EX28DRAFT_2775 0.37 -0.1 83,776 - EX28DRAFT_2775 0.59 -0.1 83,802 + EX28DRAFT_2775 0.65 +0.7 83,815 + EX28DRAFT_2775 0.68 +0.6 83,818 + EX28DRAFT_2775 0.69 +0.3 83,821 + EX28DRAFT_2775 0.69 -0.7 83,822 - EX28DRAFT_2775 0.69 -0.0 83,822 - EX28DRAFT_2775 0.69 +0.2 83,822 - EX28DRAFT_2775 0.69 +0.7 84,228 + EX28DRAFT_2776 0.49 +0.1 84,229 - EX28DRAFT_2776 0.49 +0.4 84,229 - EX28DRAFT_2776 0.49 +2.2 84,233 + EX28DRAFT_2776 0.50 +0.2 84,503 - -1.1 84,511 + -0.2 84,511 + -0.2 84,512 - -1.5 84,512 - -0.5 84,546 + -0.8 84,556 + -0.6 84,557 - +0.6 84,718 + EX28DRAFT_2777 0.64 +0.1 84,753 - +0.6 84,765 - +0.0 84,765 - +0.2 84,860 - +0.1 84,868 + +1.1 84,869 - +0.6 85,374 + +0.6
Or see this region's nucleotide sequence