Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1914 and EX28DRAFT_1915 overlap by 1 nucleotides EX28DRAFT_1915 and EX28DRAFT_1916 are separated by 11 nucleotides EX28DRAFT_1916 and EX28DRAFT_1917 are separated by 7 nucleotides EX28DRAFT_1917 and EX28DRAFT_1918 are separated by 9 nucleotides
EX28DRAFT_1914: EX28DRAFT_1914 - pyrimidine utilization protein A, at 1,925,465 to 1,926,556
_1914
EX28DRAFT_1915: EX28DRAFT_1915 - pyrimidine utilization protein B, at 1,926,556 to 1,927,245
_1915
EX28DRAFT_1916: EX28DRAFT_1916 - pyrimidine utilization protein C, at 1,927,257 to 1,927,643
_1916
EX28DRAFT_1917: EX28DRAFT_1917 - pyrimidine utilization protein D, at 1,927,651 to 1,928,451
_1917
EX28DRAFT_1918: EX28DRAFT_1918 - Nitroreductase, at 1,928,461 to 1,929,051
_1918
Position (kb) 1927
1928 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1926.386 kb on + strand, within EX28DRAFT_1914 at 1927.090 kb on + strand, within EX28DRAFT_1915 at 1927.341 kb on + strand, within EX28DRAFT_1916 at 1927.341 kb on + strand, within EX28DRAFT_1916 at 1927.342 kb on - strand, within EX28DRAFT_1916 at 1927.342 kb on - strand, within EX28DRAFT_1916 at 1927.389 kb on - strand, within EX28DRAFT_1916 at 1927.512 kb on + strand, within EX28DRAFT_1916 at 1927.533 kb on + strand, within EX28DRAFT_1916 at 1927.533 kb on + strand, within EX28DRAFT_1916 at 1927.650 kb on + strand at 1927.651 kb on - strand at 1927.704 kb on + strand at 1927.732 kb on + strand, within EX28DRAFT_1917 at 1927.733 kb on - strand, within EX28DRAFT_1917 at 1927.784 kb on - strand, within EX28DRAFT_1917 at 1928.050 kb on + strand, within EX28DRAFT_1917 at 1928.260 kb on - strand, within EX28DRAFT_1917 at 1928.260 kb on - strand, within EX28DRAFT_1917 at 1928.448 kb on - strand at 1928.449 kb on + strand at 1928.459 kb on + strand at 1928.570 kb on - strand, within EX28DRAFT_1918
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,926,386 + EX28DRAFT_1914 0.84 -4.0 1,927,090 + EX28DRAFT_1915 0.77 -0.3 1,927,341 + EX28DRAFT_1916 0.22 -0.3 1,927,341 + EX28DRAFT_1916 0.22 +1.9 1,927,342 - EX28DRAFT_1916 0.22 +0.7 1,927,342 - EX28DRAFT_1916 0.22 +1.5 1,927,389 - EX28DRAFT_1916 0.34 +0.5 1,927,512 + EX28DRAFT_1916 0.66 -0.1 1,927,533 + EX28DRAFT_1916 0.71 +0.4 1,927,533 + EX28DRAFT_1916 0.71 +0.4 1,927,650 + +0.0 1,927,651 - -0.2 1,927,704 + +0.9 1,927,732 + EX28DRAFT_1917 0.10 -0.0 1,927,733 - EX28DRAFT_1917 0.10 +0.2 1,927,784 - EX28DRAFT_1917 0.17 +1.3 1,928,050 + EX28DRAFT_1917 0.50 -0.6 1,928,260 - EX28DRAFT_1917 0.76 -1.8 1,928,260 - EX28DRAFT_1917 0.76 +0.0 1,928,448 - +0.6 1,928,449 + -0.0 1,928,459 + -0.3 1,928,570 - EX28DRAFT_1918 0.18 -0.1
Or see this region's nucleotide sequence