Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1863 and EX28DRAFT_1864 are separated by 129 nucleotides EX28DRAFT_1864 and EX28DRAFT_1865 are separated by 2 nucleotides EX28DRAFT_1865 and EX28DRAFT_1866 are separated by 9 nucleotides
EX28DRAFT_1863: EX28DRAFT_1863 - murein biosynthesis integral membrane protein MurJ, at 1,876,916 to 1,878,451
_1863
EX28DRAFT_1864: EX28DRAFT_1864 - Predicted dehydrogenases and related proteins, at 1,878,581 to 1,879,504
_1864
EX28DRAFT_1865: EX28DRAFT_1865 - Uncharacterized protein conserved in bacteria, at 1,879,507 to 1,880,163
_1865
EX28DRAFT_1866: EX28DRAFT_1866 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 1,880,173 to 1,880,757
_1866
Position (kb)
1878
1879
1880 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1878.556 kb on + strand at 1878.556 kb on + strand at 1878.556 kb on + strand at 1878.799 kb on + strand, within EX28DRAFT_1864 at 1878.915 kb on + strand, within EX28DRAFT_1864 at 1879.011 kb on + strand, within EX28DRAFT_1864 at 1879.012 kb on - strand, within EX28DRAFT_1864 at 1879.012 kb on - strand, within EX28DRAFT_1864 at 1879.294 kb on + strand, within EX28DRAFT_1864 at 1879.295 kb on - strand, within EX28DRAFT_1864 at 1879.295 kb on - strand, within EX28DRAFT_1864 at 1879.372 kb on + strand, within EX28DRAFT_1864 at 1879.372 kb on + strand, within EX28DRAFT_1864 at 1879.372 kb on + strand, within EX28DRAFT_1864 at 1879.372 kb on + strand, within EX28DRAFT_1864 at 1879.436 kb on + strand at 1879.674 kb on + strand, within EX28DRAFT_1865 at 1879.675 kb on - strand, within EX28DRAFT_1865 at 1879.675 kb on - strand, within EX28DRAFT_1865 at 1879.676 kb on + strand, within EX28DRAFT_1865 at 1879.677 kb on - strand, within EX28DRAFT_1865 at 1879.922 kb on + strand, within EX28DRAFT_1865 at 1880.157 kb on - strand at 1880.157 kb on - strand at 1880.157 kb on - strand at 1880.250 kb on - strand, within EX28DRAFT_1866 at 1880.322 kb on + strand, within EX28DRAFT_1866 at 1880.323 kb on - strand, within EX28DRAFT_1866 at 1880.324 kb on + strand, within EX28DRAFT_1866 at 1880.324 kb on + strand, within EX28DRAFT_1866 at 1880.324 kb on + strand, within EX28DRAFT_1866 at 1880.325 kb on - strand, within EX28DRAFT_1866 at 1880.347 kb on - strand, within EX28DRAFT_1866 at 1880.364 kb on + strand, within EX28DRAFT_1866 at 1880.364 kb on + strand, within EX28DRAFT_1866 at 1880.364 kb on + strand, within EX28DRAFT_1866 at 1880.364 kb on + strand, within EX28DRAFT_1866 at 1880.365 kb on - strand, within EX28DRAFT_1866 at 1880.365 kb on - strand, within EX28DRAFT_1866 at 1880.394 kb on - strand, within EX28DRAFT_1866 at 1880.442 kb on - strand, within EX28DRAFT_1866
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,878,556 + +2.2 1,878,556 + +0.3 1,878,556 + +0.8 1,878,799 + EX28DRAFT_1864 0.24 -2.9 1,878,915 + EX28DRAFT_1864 0.36 -0.4 1,879,011 + EX28DRAFT_1864 0.47 +1.2 1,879,012 - EX28DRAFT_1864 0.47 +0.1 1,879,012 - EX28DRAFT_1864 0.47 +0.2 1,879,294 + EX28DRAFT_1864 0.77 -1.1 1,879,295 - EX28DRAFT_1864 0.77 +0.5 1,879,295 - EX28DRAFT_1864 0.77 -0.1 1,879,372 + EX28DRAFT_1864 0.86 +0.8 1,879,372 + EX28DRAFT_1864 0.86 +1.6 1,879,372 + EX28DRAFT_1864 0.86 +0.4 1,879,372 + EX28DRAFT_1864 0.86 +0.7 1,879,436 + -0.6 1,879,674 + EX28DRAFT_1865 0.25 +1.5 1,879,675 - EX28DRAFT_1865 0.26 -0.2 1,879,675 - EX28DRAFT_1865 0.26 -3.5 1,879,676 + EX28DRAFT_1865 0.26 -0.2 1,879,677 - EX28DRAFT_1865 0.26 +0.7 1,879,922 + EX28DRAFT_1865 0.63 +0.1 1,880,157 - -0.3 1,880,157 - -0.0 1,880,157 - -0.6 1,880,250 - EX28DRAFT_1866 0.13 +0.3 1,880,322 + EX28DRAFT_1866 0.25 -0.3 1,880,323 - EX28DRAFT_1866 0.26 +0.0 1,880,324 + EX28DRAFT_1866 0.26 +0.2 1,880,324 + EX28DRAFT_1866 0.26 +0.7 1,880,324 + EX28DRAFT_1866 0.26 +0.4 1,880,325 - EX28DRAFT_1866 0.26 +0.3 1,880,347 - EX28DRAFT_1866 0.30 -0.2 1,880,364 + EX28DRAFT_1866 0.33 +0.1 1,880,364 + EX28DRAFT_1866 0.33 -0.6 1,880,364 + EX28DRAFT_1866 0.33 +0.2 1,880,364 + EX28DRAFT_1866 0.33 +0.8 1,880,365 - EX28DRAFT_1866 0.33 +0.9 1,880,365 - EX28DRAFT_1866 0.33 +1.0 1,880,394 - EX28DRAFT_1866 0.38 +0.5 1,880,442 - EX28DRAFT_1866 0.46 +0.2
Or see this region's nucleotide sequence