Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1817 and EX28DRAFT_1818 are separated by 75 nucleotides EX28DRAFT_1818 and EX28DRAFT_1819 are separated by 244 nucleotides EX28DRAFT_1819 and EX28DRAFT_1820 are separated by 59 nucleotides EX28DRAFT_1820 and EX28DRAFT_1821 are separated by 193 nucleotides
EX28DRAFT_1817: EX28DRAFT_1817 - Uncharacterized protein conserved in bacteria, at 1,836,609 to 1,837,574
_1817
EX28DRAFT_1818: EX28DRAFT_1818 - Protein of unknown function (DUF1471), at 1,837,650 to 1,837,907
_1818
EX28DRAFT_1819: EX28DRAFT_1819 - transcriptional regulator, TetR family, at 1,838,152 to 1,838,787
_1819
EX28DRAFT_1820: EX28DRAFT_1820 - Predicted outer membrane lipoprotein, at 1,838,847 to 1,839,386
_1820
EX28DRAFT_1821: EX28DRAFT_1821 - NADH dehydrogenase, FAD-containing subunit, at 1,839,580 to 1,840,884
_1821
Position (kb)
1838
1839 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1837.200 kb on + strand, within EX28DRAFT_1817 at 1837.259 kb on + strand, within EX28DRAFT_1817 at 1837.260 kb on - strand, within EX28DRAFT_1817 at 1837.260 kb on - strand, within EX28DRAFT_1817 at 1837.295 kb on + strand, within EX28DRAFT_1817 at 1837.326 kb on - strand, within EX28DRAFT_1817 at 1837.328 kb on + strand, within EX28DRAFT_1817 at 1837.334 kb on + strand, within EX28DRAFT_1817 at 1837.335 kb on - strand, within EX28DRAFT_1817 at 1837.457 kb on - strand, within EX28DRAFT_1817 at 1837.457 kb on - strand, within EX28DRAFT_1817 at 1837.616 kb on - strand at 1837.652 kb on - strand at 1837.657 kb on + strand at 1837.662 kb on - strand at 1837.686 kb on - strand, within EX28DRAFT_1818 at 1837.707 kb on - strand, within EX28DRAFT_1818 at 1837.772 kb on + strand, within EX28DRAFT_1818 at 1837.793 kb on + strand, within EX28DRAFT_1818 at 1837.794 kb on - strand, within EX28DRAFT_1818 at 1837.805 kb on + strand, within EX28DRAFT_1818 at 1837.805 kb on + strand, within EX28DRAFT_1818 at 1837.836 kb on + strand, within EX28DRAFT_1818 at 1837.836 kb on + strand, within EX28DRAFT_1818 at 1837.914 kb on + strand at 1837.914 kb on + strand at 1837.915 kb on - strand at 1837.915 kb on - strand at 1837.966 kb on - strand at 1838.058 kb on - strand at 1838.288 kb on + strand, within EX28DRAFT_1819 at 1838.372 kb on - strand, within EX28DRAFT_1819 at 1838.372 kb on - strand, within EX28DRAFT_1819 at 1838.372 kb on - strand, within EX28DRAFT_1819 at 1838.505 kb on + strand, within EX28DRAFT_1819 at 1838.668 kb on + strand, within EX28DRAFT_1819 at 1838.740 kb on - strand at 1838.827 kb on - strand at 1838.827 kb on - strand at 1838.827 kb on - strand at 1838.827 kb on - strand at 1838.858 kb on + strand at 1838.989 kb on + strand, within EX28DRAFT_1820 at 1839.022 kb on + strand, within EX28DRAFT_1820 at 1839.023 kb on - strand, within EX28DRAFT_1820 at 1839.026 kb on + strand, within EX28DRAFT_1820 at 1839.100 kb on - strand, within EX28DRAFT_1820 at 1839.286 kb on + strand, within EX28DRAFT_1820 at 1839.287 kb on - strand, within EX28DRAFT_1820 at 1839.303 kb on + strand, within EX28DRAFT_1820 at 1839.306 kb on + strand, within EX28DRAFT_1820 at 1839.361 kb on - strand at 1839.361 kb on - strand at 1839.484 kb on + strand at 1839.484 kb on + strand at 1839.485 kb on - strand at 1839.485 kb on - strand at 1839.486 kb on + strand at 1839.486 kb on + strand at 1839.492 kb on - strand at 1839.495 kb on + strand at 1839.495 kb on + strand at 1839.495 kb on + strand at 1839.507 kb on + strand at 1839.507 kb on + strand at 1839.654 kb on - strand at 1839.693 kb on + strand at 1839.695 kb on + strand at 1839.695 kb on + strand at 1839.695 kb on + strand at 1839.696 kb on - strand at 1839.696 kb on - strand at 1839.721 kb on + strand, within EX28DRAFT_1821 at 1839.722 kb on - strand, within EX28DRAFT_1821 at 1839.722 kb on - strand, within EX28DRAFT_1821
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,837,200 + EX28DRAFT_1817 0.61 -1.1 1,837,259 + EX28DRAFT_1817 0.67 -0.1 1,837,260 - EX28DRAFT_1817 0.67 -0.1 1,837,260 - EX28DRAFT_1817 0.67 +0.8 1,837,295 + EX28DRAFT_1817 0.71 -0.1 1,837,326 - EX28DRAFT_1817 0.74 +0.3 1,837,328 + EX28DRAFT_1817 0.74 -0.2 1,837,334 + EX28DRAFT_1817 0.75 +0.2 1,837,335 - EX28DRAFT_1817 0.75 +0.1 1,837,457 - EX28DRAFT_1817 0.88 -0.6 1,837,457 - EX28DRAFT_1817 0.88 +1.0 1,837,616 - +0.4 1,837,652 - +0.7 1,837,657 + +0.3 1,837,662 - +0.8 1,837,686 - EX28DRAFT_1818 0.14 -0.6 1,837,707 - EX28DRAFT_1818 0.22 -0.6 1,837,772 + EX28DRAFT_1818 0.47 +0.1 1,837,793 + EX28DRAFT_1818 0.55 +0.1 1,837,794 - EX28DRAFT_1818 0.56 -3.2 1,837,805 + EX28DRAFT_1818 0.60 +0.2 1,837,805 + EX28DRAFT_1818 0.60 +1.5 1,837,836 + EX28DRAFT_1818 0.72 +0.1 1,837,836 + EX28DRAFT_1818 0.72 +0.4 1,837,914 + +0.2 1,837,914 + +1.3 1,837,915 - +1.0 1,837,915 - +1.0 1,837,966 - +1.2 1,838,058 - +0.2 1,838,288 + EX28DRAFT_1819 0.21 +0.1 1,838,372 - EX28DRAFT_1819 0.35 +0.6 1,838,372 - EX28DRAFT_1819 0.35 +1.4 1,838,372 - EX28DRAFT_1819 0.35 +0.1 1,838,505 + EX28DRAFT_1819 0.56 -1.4 1,838,668 + EX28DRAFT_1819 0.81 +0.5 1,838,740 - -0.2 1,838,827 - -0.2 1,838,827 - +0.6 1,838,827 - +0.6 1,838,827 - -1.7 1,838,858 + -0.1 1,838,989 + EX28DRAFT_1820 0.26 +1.0 1,839,022 + EX28DRAFT_1820 0.32 +1.0 1,839,023 - EX28DRAFT_1820 0.33 -2.2 1,839,026 + EX28DRAFT_1820 0.33 +0.8 1,839,100 - EX28DRAFT_1820 0.47 +0.2 1,839,286 + EX28DRAFT_1820 0.81 -0.4 1,839,287 - EX28DRAFT_1820 0.81 -0.6 1,839,303 + EX28DRAFT_1820 0.84 +0.4 1,839,306 + EX28DRAFT_1820 0.85 -0.8 1,839,361 - +1.0 1,839,361 - -0.5 1,839,484 + -0.3 1,839,484 + -1.6 1,839,485 - +1.3 1,839,485 - +0.1 1,839,486 + -3.9 1,839,486 + +0.2 1,839,492 - +0.2 1,839,495 + -0.8 1,839,495 + -0.3 1,839,495 + -0.3 1,839,507 + +0.9 1,839,507 + +0.2 1,839,654 - +0.5 1,839,693 + -1.2 1,839,695 + +0.3 1,839,695 + +1.9 1,839,695 + +0.4 1,839,696 - -2.9 1,839,696 - -2.1 1,839,721 + EX28DRAFT_1821 0.11 +0.3 1,839,722 - EX28DRAFT_1821 0.11 -2.6 1,839,722 - EX28DRAFT_1821 0.11 -0.5
Or see this region's nucleotide sequence