Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1803 and EX28DRAFT_1804 are separated by 300 nucleotides EX28DRAFT_1804 and EX28DRAFT_1805 overlap by 17 nucleotides EX28DRAFT_1805 and EX28DRAFT_1806 overlap by 4 nucleotides
EX28DRAFT_1803: EX28DRAFT_1803 - peptidase T, at 1,817,402 to 1,818,625
_1803
EX28DRAFT_1804: EX28DRAFT_1804 - spermidine/putrescine ABC transporter ATP-binding subunit, at 1,818,926 to 1,820,011
_1804
EX28DRAFT_1805: EX28DRAFT_1805 - ABC-type spermidine/putrescine transport system, permease component I, at 1,819,995 to 1,820,852
_1805
EX28DRAFT_1806: EX28DRAFT_1806 - ABC-type spermidine/putrescine transport system, permease component II, at 1,820,849 to 1,821,640
_1806
Position (kb)
1818
1819
1820
1821 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1818.048 kb on + strand, within EX28DRAFT_1803 at 1818.365 kb on - strand, within EX28DRAFT_1803 at 1818.521 kb on + strand at 1818.572 kb on - strand at 1818.572 kb on - strand at 1818.572 kb on - strand at 1818.594 kb on + strand at 1818.615 kb on - strand at 1818.619 kb on + strand at 1818.620 kb on - strand at 1818.728 kb on + strand at 1818.729 kb on - strand at 1818.745 kb on - strand at 1818.750 kb on + strand at 1818.751 kb on - strand at 1818.756 kb on - strand at 1818.834 kb on + strand at 1818.834 kb on + strand at 1818.874 kb on - strand at 1819.150 kb on + strand, within EX28DRAFT_1804 at 1819.167 kb on - strand, within EX28DRAFT_1804 at 1819.445 kb on - strand, within EX28DRAFT_1804 at 1819.445 kb on - strand, within EX28DRAFT_1804 at 1819.581 kb on - strand, within EX28DRAFT_1804 at 1819.630 kb on + strand, within EX28DRAFT_1804 at 1819.631 kb on - strand, within EX28DRAFT_1804 at 1819.705 kb on + strand, within EX28DRAFT_1804 at 1819.839 kb on - strand, within EX28DRAFT_1804 at 1820.152 kb on - strand, within EX28DRAFT_1805 at 1820.152 kb on - strand, within EX28DRAFT_1805 at 1820.379 kb on + strand, within EX28DRAFT_1805 at 1820.417 kb on + strand, within EX28DRAFT_1805 at 1820.469 kb on + strand, within EX28DRAFT_1805 at 1820.469 kb on + strand, within EX28DRAFT_1805 at 1820.469 kb on + strand, within EX28DRAFT_1805 at 1820.470 kb on - strand, within EX28DRAFT_1805 at 1820.470 kb on - strand, within EX28DRAFT_1805 at 1820.470 kb on - strand, within EX28DRAFT_1805 at 1820.591 kb on + strand, within EX28DRAFT_1805 at 1820.637 kb on - strand, within EX28DRAFT_1805 at 1820.661 kb on + strand, within EX28DRAFT_1805 at 1820.831 kb on + strand at 1820.892 kb on - strand at 1820.897 kb on + strand at 1820.898 kb on - strand at 1820.898 kb on - strand at 1820.903 kb on + strand at 1820.903 kb on + strand at 1820.903 kb on + strand at 1820.903 kb on + strand at 1820.960 kb on - strand, within EX28DRAFT_1806 at 1820.975 kb on - strand, within EX28DRAFT_1806 at 1820.987 kb on + strand, within EX28DRAFT_1806
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,818,048 + EX28DRAFT_1803 0.53 -3.3 1,818,365 - EX28DRAFT_1803 0.79 +0.4 1,818,521 + +0.4 1,818,572 - +1.6 1,818,572 - +0.9 1,818,572 - +0.1 1,818,594 + -0.7 1,818,615 - -2.2 1,818,619 + +3.0 1,818,620 - +0.6 1,818,728 + -0.2 1,818,729 - -1.8 1,818,745 - -0.0 1,818,750 + +0.0 1,818,751 - +0.6 1,818,756 - +1.0 1,818,834 + -1.7 1,818,834 + +0.7 1,818,874 - +0.4 1,819,150 + EX28DRAFT_1804 0.21 +2.0 1,819,167 - EX28DRAFT_1804 0.22 -0.6 1,819,445 - EX28DRAFT_1804 0.48 +0.7 1,819,445 - EX28DRAFT_1804 0.48 +1.0 1,819,581 - EX28DRAFT_1804 0.60 +1.4 1,819,630 + EX28DRAFT_1804 0.65 -0.9 1,819,631 - EX28DRAFT_1804 0.65 +0.3 1,819,705 + EX28DRAFT_1804 0.72 +1.3 1,819,839 - EX28DRAFT_1804 0.84 +0.5 1,820,152 - EX28DRAFT_1805 0.18 +0.1 1,820,152 - EX28DRAFT_1805 0.18 -1.7 1,820,379 + EX28DRAFT_1805 0.45 +0.3 1,820,417 + EX28DRAFT_1805 0.49 -2.9 1,820,469 + EX28DRAFT_1805 0.55 -3.2 1,820,469 + EX28DRAFT_1805 0.55 +0.8 1,820,469 + EX28DRAFT_1805 0.55 +0.2 1,820,470 - EX28DRAFT_1805 0.55 +1.1 1,820,470 - EX28DRAFT_1805 0.55 +0.4 1,820,470 - EX28DRAFT_1805 0.55 -0.0 1,820,591 + EX28DRAFT_1805 0.69 -0.5 1,820,637 - EX28DRAFT_1805 0.75 -1.6 1,820,661 + EX28DRAFT_1805 0.78 +0.9 1,820,831 + -0.2 1,820,892 - +0.4 1,820,897 + +0.8 1,820,898 - -0.2 1,820,898 - +0.2 1,820,903 + -0.4 1,820,903 + +0.5 1,820,903 + +0.3 1,820,903 + +0.5 1,820,960 - EX28DRAFT_1806 0.14 -0.4 1,820,975 - EX28DRAFT_1806 0.16 +2.1 1,820,987 + EX28DRAFT_1806 0.17 +0.2
Or see this region's nucleotide sequence