Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1681 and EX28DRAFT_1682 are separated by 97 nucleotides EX28DRAFT_1682 and EX28DRAFT_1683 are separated by 14 nucleotides EX28DRAFT_1683 and EX28DRAFT_1684 overlap by 4 nucleotides
EX28DRAFT_1681: EX28DRAFT_1681 - Uncharacterized protein conserved in bacteria, at 1,693,680 to 1,694,693
_1681
EX28DRAFT_1682: EX28DRAFT_1682 - SufE protein probably involved in Fe-S center assembly, at 1,694,791 to 1,695,207
_1682
EX28DRAFT_1683: EX28DRAFT_1683 - cysteine desulfurases, SufSfamily, at 1,695,222 to 1,696,442
_1683
EX28DRAFT_1684: EX28DRAFT_1684 - FeS assembly protein SufD, at 1,696,439 to 1,697,710
_1684
Position (kb)
1695
1696
1697 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1694.365 kb on + strand, within EX28DRAFT_1681 at 1694.366 kb on - strand, within EX28DRAFT_1681 at 1694.366 kb on - strand, within EX28DRAFT_1681 at 1694.366 kb on - strand, within EX28DRAFT_1681 at 1694.391 kb on + strand, within EX28DRAFT_1681 at 1694.483 kb on + strand, within EX28DRAFT_1681 at 1694.503 kb on - strand, within EX28DRAFT_1681 at 1694.503 kb on - strand, within EX28DRAFT_1681 at 1694.546 kb on + strand, within EX28DRAFT_1681 at 1694.547 kb on - strand, within EX28DRAFT_1681 at 1694.569 kb on + strand, within EX28DRAFT_1681 at 1694.639 kb on + strand at 1694.667 kb on + strand at 1694.667 kb on + strand at 1694.668 kb on - strand at 1694.668 kb on - strand at 1694.673 kb on + strand at 1694.673 kb on + strand at 1694.673 kb on + strand at 1694.674 kb on - strand at 1694.674 kb on - strand at 1694.676 kb on + strand at 1694.676 kb on + strand at 1694.787 kb on - strand at 1694.792 kb on + strand at 1694.792 kb on + strand at 1694.793 kb on - strand at 1694.796 kb on + strand at 1694.950 kb on - strand, within EX28DRAFT_1682 at 1694.974 kb on + strand, within EX28DRAFT_1682 at 1694.974 kb on + strand, within EX28DRAFT_1682 at 1695.129 kb on + strand, within EX28DRAFT_1682 at 1695.130 kb on - strand, within EX28DRAFT_1682 at 1695.130 kb on - strand, within EX28DRAFT_1682 at 1695.130 kb on - strand, within EX28DRAFT_1682 at 1695.131 kb on + strand, within EX28DRAFT_1682 at 1695.132 kb on - strand, within EX28DRAFT_1682 at 1695.132 kb on - strand, within EX28DRAFT_1682 at 1695.132 kb on - strand, within EX28DRAFT_1682 at 1695.132 kb on - strand, within EX28DRAFT_1682 at 1695.137 kb on + strand, within EX28DRAFT_1682 at 1695.138 kb on - strand, within EX28DRAFT_1682 at 1695.138 kb on - strand, within EX28DRAFT_1682 at 1695.243 kb on - strand at 1695.289 kb on + strand at 1695.290 kb on - strand at 1695.328 kb on + strand at 1695.329 kb on - strand at 1695.383 kb on - strand, within EX28DRAFT_1683 at 1695.409 kb on + strand, within EX28DRAFT_1683 at 1695.409 kb on + strand, within EX28DRAFT_1683 at 1695.542 kb on + strand, within EX28DRAFT_1683 at 1695.542 kb on + strand, within EX28DRAFT_1683 at 1695.543 kb on - strand, within EX28DRAFT_1683 at 1695.565 kb on + strand, within EX28DRAFT_1683 at 1695.565 kb on + strand, within EX28DRAFT_1683 at 1695.565 kb on + strand, within EX28DRAFT_1683 at 1695.566 kb on - strand, within EX28DRAFT_1683 at 1695.566 kb on - strand, within EX28DRAFT_1683 at 1695.666 kb on + strand, within EX28DRAFT_1683 at 1696.136 kb on + strand, within EX28DRAFT_1683 at 1696.142 kb on + strand, within EX28DRAFT_1683 at 1696.143 kb on - strand, within EX28DRAFT_1683 at 1696.265 kb on + strand, within EX28DRAFT_1683 at 1696.272 kb on - strand, within EX28DRAFT_1683 at 1696.303 kb on + strand, within EX28DRAFT_1683 at 1696.366 kb on + strand at 1696.367 kb on - strand at 1696.539 kb on + strand at 1696.539 kb on + strand at 1696.539 kb on + strand at 1696.543 kb on + strand at 1696.544 kb on - strand at 1696.590 kb on + strand, within EX28DRAFT_1684 at 1696.590 kb on + strand, within EX28DRAFT_1684 at 1696.807 kb on + strand, within EX28DRAFT_1684 at 1696.986 kb on + strand, within EX28DRAFT_1684 at 1696.986 kb on + strand, within EX28DRAFT_1684 at 1696.987 kb on - strand, within EX28DRAFT_1684 at 1697.010 kb on - strand, within EX28DRAFT_1684
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,694,365 + EX28DRAFT_1681 0.68 -1.0 1,694,366 - EX28DRAFT_1681 0.68 +2.1 1,694,366 - EX28DRAFT_1681 0.68 -0.6 1,694,366 - EX28DRAFT_1681 0.68 -1.7 1,694,391 + EX28DRAFT_1681 0.70 -0.2 1,694,483 + EX28DRAFT_1681 0.79 +0.2 1,694,503 - EX28DRAFT_1681 0.81 -0.9 1,694,503 - EX28DRAFT_1681 0.81 +0.2 1,694,546 + EX28DRAFT_1681 0.85 +0.4 1,694,547 - EX28DRAFT_1681 0.86 +0.6 1,694,569 + EX28DRAFT_1681 0.88 -0.3 1,694,639 + +0.2 1,694,667 + -0.2 1,694,667 + -0.3 1,694,668 - +0.1 1,694,668 - +0.6 1,694,673 + -0.8 1,694,673 + +0.7 1,694,673 + +0.2 1,694,674 - +1.3 1,694,674 - +0.4 1,694,676 + +0.8 1,694,676 + -0.1 1,694,787 - +0.9 1,694,792 + +0.4 1,694,792 + -0.1 1,694,793 - +1.2 1,694,796 + +0.6 1,694,950 - EX28DRAFT_1682 0.38 +0.4 1,694,974 + EX28DRAFT_1682 0.44 -1.0 1,694,974 + EX28DRAFT_1682 0.44 +0.7 1,695,129 + EX28DRAFT_1682 0.81 -0.4 1,695,130 - EX28DRAFT_1682 0.81 -0.5 1,695,130 - EX28DRAFT_1682 0.81 +0.0 1,695,130 - EX28DRAFT_1682 0.81 -0.9 1,695,131 + EX28DRAFT_1682 0.82 +0.4 1,695,132 - EX28DRAFT_1682 0.82 +1.5 1,695,132 - EX28DRAFT_1682 0.82 +0.2 1,695,132 - EX28DRAFT_1682 0.82 +0.1 1,695,132 - EX28DRAFT_1682 0.82 +1.1 1,695,137 + EX28DRAFT_1682 0.83 +1.5 1,695,138 - EX28DRAFT_1682 0.83 -2.0 1,695,138 - EX28DRAFT_1682 0.83 +0.1 1,695,243 - -0.2 1,695,289 + +1.2 1,695,290 - +0.7 1,695,328 + -0.2 1,695,329 - +0.6 1,695,383 - EX28DRAFT_1683 0.13 +0.3 1,695,409 + EX28DRAFT_1683 0.15 +0.6 1,695,409 + EX28DRAFT_1683 0.15 +0.5 1,695,542 + EX28DRAFT_1683 0.26 -0.3 1,695,542 + EX28DRAFT_1683 0.26 -0.1 1,695,543 - EX28DRAFT_1683 0.26 +0.5 1,695,565 + EX28DRAFT_1683 0.28 +0.5 1,695,565 + EX28DRAFT_1683 0.28 +0.3 1,695,565 + EX28DRAFT_1683 0.28 +1.3 1,695,566 - EX28DRAFT_1683 0.28 +0.9 1,695,566 - EX28DRAFT_1683 0.28 +0.5 1,695,666 + EX28DRAFT_1683 0.36 -0.2 1,696,136 + EX28DRAFT_1683 0.75 -2.3 1,696,142 + EX28DRAFT_1683 0.75 +0.9 1,696,143 - EX28DRAFT_1683 0.75 -0.3 1,696,265 + EX28DRAFT_1683 0.85 -3.7 1,696,272 - EX28DRAFT_1683 0.86 +1.7 1,696,303 + EX28DRAFT_1683 0.89 +1.0 1,696,366 + -0.9 1,696,367 - +0.7 1,696,539 + -0.5 1,696,539 + -0.7 1,696,539 + -2.7 1,696,543 + +0.1 1,696,544 - +1.3 1,696,590 + EX28DRAFT_1684 0.12 +0.7 1,696,590 + EX28DRAFT_1684 0.12 +0.6 1,696,807 + EX28DRAFT_1684 0.29 +0.4 1,696,986 + EX28DRAFT_1684 0.43 -0.4 1,696,986 + EX28DRAFT_1684 0.43 +0.4 1,696,987 - EX28DRAFT_1684 0.43 +1.0 1,697,010 - EX28DRAFT_1684 0.45 +1.1
Or see this region's nucleotide sequence