Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1501 and EX28DRAFT_1502 overlap by 4 nucleotides EX28DRAFT_1502 and EX28DRAFT_1503 are separated by 24 nucleotides
EX28DRAFT_1501: EX28DRAFT_1501 - NAD-dependent aldehyde dehydrogenases, at 1,515,697 to 1,517,190
_1501
EX28DRAFT_1502: EX28DRAFT_1502 - Glycine/D-amino acid oxidases (deaminating), at 1,517,187 to 1,518,467
_1502
EX28DRAFT_1503: EX28DRAFT_1503 - 4-aminobutyrate aminotransferase, prokaryotic type, at 1,518,492 to 1,519,763
_1503
Position (kb)
1517
1518
1519 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1516.521 kb on + strand, within EX28DRAFT_1501 at 1516.521 kb on + strand, within EX28DRAFT_1501 at 1516.522 kb on - strand, within EX28DRAFT_1501 at 1516.522 kb on - strand, within EX28DRAFT_1501 at 1516.522 kb on - strand, within EX28DRAFT_1501 at 1517.052 kb on + strand at 1517.052 kb on + strand at 1517.056 kb on + strand at 1517.056 kb on + strand at 1517.056 kb on + strand at 1517.070 kb on + strand at 1517.070 kb on + strand at 1517.198 kb on + strand at 1517.232 kb on + strand at 1517.415 kb on + strand, within EX28DRAFT_1502 at 1517.416 kb on - strand, within EX28DRAFT_1502 at 1517.526 kb on + strand, within EX28DRAFT_1502 at 1517.895 kb on + strand, within EX28DRAFT_1502 at 1517.896 kb on - strand, within EX28DRAFT_1502 at 1517.907 kb on - strand, within EX28DRAFT_1502 at 1518.009 kb on - strand, within EX28DRAFT_1502 at 1518.012 kb on + strand, within EX28DRAFT_1502 at 1518.012 kb on + strand, within EX28DRAFT_1502 at 1518.013 kb on - strand, within EX28DRAFT_1502 at 1518.033 kb on + strand, within EX28DRAFT_1502 at 1518.033 kb on + strand, within EX28DRAFT_1502 at 1518.045 kb on + strand, within EX28DRAFT_1502 at 1518.045 kb on + strand, within EX28DRAFT_1502 at 1518.078 kb on + strand, within EX28DRAFT_1502 at 1518.097 kb on - strand, within EX28DRAFT_1502 at 1518.224 kb on + strand, within EX28DRAFT_1502 at 1518.258 kb on + strand, within EX28DRAFT_1502 at 1518.258 kb on + strand, within EX28DRAFT_1502 at 1518.258 kb on + strand, within EX28DRAFT_1502 at 1518.259 kb on - strand, within EX28DRAFT_1502 at 1518.261 kb on + strand, within EX28DRAFT_1502 at 1518.262 kb on - strand, within EX28DRAFT_1502 at 1518.262 kb on - strand, within EX28DRAFT_1502 at 1518.262 kb on - strand, within EX28DRAFT_1502 at 1518.616 kb on - strand at 1518.618 kb on - strand at 1518.744 kb on + strand, within EX28DRAFT_1503 at 1518.915 kb on + strand, within EX28DRAFT_1503 at 1518.915 kb on + strand, within EX28DRAFT_1503 at 1518.915 kb on + strand, within EX28DRAFT_1503 at 1518.915 kb on + strand, within EX28DRAFT_1503 at 1518.916 kb on - strand, within EX28DRAFT_1503 at 1518.916 kb on - strand, within EX28DRAFT_1503 at 1518.917 kb on + strand, within EX28DRAFT_1503 at 1518.918 kb on - strand, within EX28DRAFT_1503 at 1518.954 kb on - strand, within EX28DRAFT_1503 at 1518.988 kb on - strand, within EX28DRAFT_1503 at 1519.381 kb on - strand, within EX28DRAFT_1503 at 1519.427 kb on + strand, within EX28DRAFT_1503 at 1519.427 kb on + strand, within EX28DRAFT_1503 at 1519.428 kb on - strand, within EX28DRAFT_1503 at 1519.428 kb on - strand, within EX28DRAFT_1503
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,516,521 + EX28DRAFT_1501 0.55 -0.7 1,516,521 + EX28DRAFT_1501 0.55 -0.5 1,516,522 - EX28DRAFT_1501 0.55 -4.6 1,516,522 - EX28DRAFT_1501 0.55 -0.8 1,516,522 - EX28DRAFT_1501 0.55 +2.2 1,517,052 + -3.6 1,517,052 + +1.7 1,517,056 + -0.3 1,517,056 + +1.3 1,517,056 + +0.2 1,517,070 + +0.3 1,517,070 + -1.6 1,517,198 + +1.3 1,517,232 + -0.8 1,517,415 + EX28DRAFT_1502 0.18 -0.6 1,517,416 - EX28DRAFT_1502 0.18 -0.4 1,517,526 + EX28DRAFT_1502 0.26 +0.3 1,517,895 + EX28DRAFT_1502 0.55 -0.1 1,517,896 - EX28DRAFT_1502 0.55 -0.7 1,517,907 - EX28DRAFT_1502 0.56 -1.1 1,518,009 - EX28DRAFT_1502 0.64 +1.6 1,518,012 + EX28DRAFT_1502 0.64 +0.4 1,518,012 + EX28DRAFT_1502 0.64 -0.8 1,518,013 - EX28DRAFT_1502 0.64 +0.7 1,518,033 + EX28DRAFT_1502 0.66 +0.5 1,518,033 + EX28DRAFT_1502 0.66 -0.9 1,518,045 + EX28DRAFT_1502 0.67 -0.3 1,518,045 + EX28DRAFT_1502 0.67 -0.4 1,518,078 + EX28DRAFT_1502 0.70 -0.0 1,518,097 - EX28DRAFT_1502 0.71 -0.9 1,518,224 + EX28DRAFT_1502 0.81 +1.4 1,518,258 + EX28DRAFT_1502 0.84 -0.2 1,518,258 + EX28DRAFT_1502 0.84 +0.3 1,518,258 + EX28DRAFT_1502 0.84 -1.8 1,518,259 - EX28DRAFT_1502 0.84 -0.3 1,518,261 + EX28DRAFT_1502 0.84 +1.5 1,518,262 - EX28DRAFT_1502 0.84 +0.2 1,518,262 - EX28DRAFT_1502 0.84 +0.3 1,518,262 - EX28DRAFT_1502 0.84 -0.8 1,518,616 - +0.4 1,518,618 - -0.3 1,518,744 + EX28DRAFT_1503 0.20 -0.1 1,518,915 + EX28DRAFT_1503 0.33 -1.4 1,518,915 + EX28DRAFT_1503 0.33 -0.3 1,518,915 + EX28DRAFT_1503 0.33 -0.2 1,518,915 + EX28DRAFT_1503 0.33 -0.4 1,518,916 - EX28DRAFT_1503 0.33 +0.4 1,518,916 - EX28DRAFT_1503 0.33 -0.4 1,518,917 + EX28DRAFT_1503 0.33 +0.4 1,518,918 - EX28DRAFT_1503 0.33 -1.1 1,518,954 - EX28DRAFT_1503 0.36 +0.2 1,518,988 - EX28DRAFT_1503 0.39 +0.4 1,519,381 - EX28DRAFT_1503 0.70 -0.7 1,519,427 + EX28DRAFT_1503 0.74 -0.0 1,519,427 + EX28DRAFT_1503 0.74 -0.8 1,519,428 - EX28DRAFT_1503 0.74 -0.7 1,519,428 - EX28DRAFT_1503 0.74 +2.3
Or see this region's nucleotide sequence