Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1413 and EX28DRAFT_1414 are separated by 1 nucleotides EX28DRAFT_1414 and EX28DRAFT_1415 are separated by 2 nucleotides EX28DRAFT_1415 and EX28DRAFT_1416 overlap by 4 nucleotides
EX28DRAFT_1413: EX28DRAFT_1413 - 3-hydroxyacyl-CoA dehydrogenase PaaC, at 1,421,317 to 1,422,741
_1413
EX28DRAFT_1414: EX28DRAFT_1414 - phenylacetate degradation probable enoyl-CoA hydratase paaB, at 1,422,743 to 1,423,528
_1414
EX28DRAFT_1415: EX28DRAFT_1415 - Enoyl-CoA hydratase/carnithine racemase, at 1,423,531 to 1,424,298
_1415
EX28DRAFT_1416: EX28DRAFT_1416 - phenylacetate-CoA oxygenase/reductase, PaaK subunit, at 1,424,295 to 1,425,365
_1416
Position (kb) 1423
1424
1425 Strain fitness (log2 ratio) -2
-1
0
1 at 1422.644 kb on + strand at 1422.730 kb on + strand at 1422.731 kb on - strand at 1422.731 kb on - strand at 1422.744 kb on + strand at 1422.745 kb on - strand at 1422.956 kb on - strand, within EX28DRAFT_1414 at 1423.267 kb on + strand, within EX28DRAFT_1414 at 1423.615 kb on - strand, within EX28DRAFT_1415 at 1423.813 kb on - strand, within EX28DRAFT_1415 at 1423.896 kb on + strand, within EX28DRAFT_1415 at 1424.402 kb on - strand, within EX28DRAFT_1416 at 1424.445 kb on - strand, within EX28DRAFT_1416 at 1424.633 kb on + strand, within EX28DRAFT_1416 at 1424.863 kb on + strand, within EX28DRAFT_1416 at 1425.023 kb on - strand, within EX28DRAFT_1416 at 1425.078 kb on + strand, within EX28DRAFT_1416 at 1425.079 kb on - strand, within EX28DRAFT_1416 at 1425.196 kb on + strand, within EX28DRAFT_1416 at 1425.196 kb on + strand, within EX28DRAFT_1416 at 1425.197 kb on - strand, within EX28DRAFT_1416
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,422,644 + -1.9 1,422,730 + -0.7 1,422,731 - +0.4 1,422,731 - -0.1 1,422,744 + -0.3 1,422,745 - -1.4 1,422,956 - EX28DRAFT_1414 0.27 -2.3 1,423,267 + EX28DRAFT_1414 0.67 -0.4 1,423,615 - EX28DRAFT_1415 0.11 +0.4 1,423,813 - EX28DRAFT_1415 0.37 +0.9 1,423,896 + EX28DRAFT_1415 0.48 +0.2 1,424,402 - EX28DRAFT_1416 0.10 +0.6 1,424,445 - EX28DRAFT_1416 0.14 +0.4 1,424,633 + EX28DRAFT_1416 0.32 +0.7 1,424,863 + EX28DRAFT_1416 0.53 +0.2 1,425,023 - EX28DRAFT_1416 0.68 +0.0 1,425,078 + EX28DRAFT_1416 0.73 -1.9 1,425,079 - EX28DRAFT_1416 0.73 -1.1 1,425,196 + EX28DRAFT_1416 0.84 +0.3 1,425,196 + EX28DRAFT_1416 0.84 -0.9 1,425,197 - EX28DRAFT_1416 0.84 -0.3
Or see this region's nucleotide sequence