Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1064 and EX28DRAFT_1065 overlap by 8 nucleotides EX28DRAFT_1065 and EX28DRAFT_1066 are separated by 160 nucleotides EX28DRAFT_1066 and EX28DRAFT_1067 are separated by 35 nucleotides EX28DRAFT_1067 and EX28DRAFT_1068 are separated by 108 nucleotides
EX28DRAFT_1064: EX28DRAFT_1064 - Helix-turn-helix domain, at 1,075,862 to 1,076,503
_1064
EX28DRAFT_1065: EX28DRAFT_1065 - Predicted transcriptional regulators, at 1,076,496 to 1,076,795
_1065
EX28DRAFT_1066: EX28DRAFT_1066 - translation elongation factor EF-G, at 1,076,956 to 1,079,055
_1066
EX28DRAFT_1067: EX28DRAFT_1067 - Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family, at 1,079,091 to 1,079,663
_1067
EX28DRAFT_1068: EX28DRAFT_1068 - Arylamine N-acetyltransferase, at 1,079,772 to 1,080,617
_1068
Position (kb)
1076
1077
1078
1079
1080 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1076.021 kb on + strand, within EX28DRAFT_1064 at 1076.080 kb on - strand, within EX28DRAFT_1064 at 1076.153 kb on + strand, within EX28DRAFT_1064 at 1076.154 kb on - strand, within EX28DRAFT_1064 at 1076.194 kb on + strand, within EX28DRAFT_1064 at 1076.198 kb on + strand, within EX28DRAFT_1064 at 1076.198 kb on + strand, within EX28DRAFT_1064 at 1076.199 kb on - strand, within EX28DRAFT_1064 at 1076.199 kb on - strand, within EX28DRAFT_1064 at 1076.242 kb on + strand, within EX28DRAFT_1064 at 1076.243 kb on - strand, within EX28DRAFT_1064 at 1076.243 kb on - strand, within EX28DRAFT_1064 at 1076.255 kb on + strand, within EX28DRAFT_1064 at 1076.514 kb on + strand at 1076.514 kb on + strand at 1076.653 kb on - strand, within EX28DRAFT_1065 at 1076.653 kb on - strand, within EX28DRAFT_1065 at 1076.763 kb on + strand, within EX28DRAFT_1065 at 1076.827 kb on - strand at 1076.857 kb on - strand at 1076.952 kb on + strand at 1077.053 kb on - strand at 1077.083 kb on + strand at 1077.084 kb on - strand at 1077.190 kb on + strand, within EX28DRAFT_1066 at 1077.292 kb on + strand, within EX28DRAFT_1066 at 1077.293 kb on - strand, within EX28DRAFT_1066 at 1077.293 kb on - strand, within EX28DRAFT_1066 at 1077.293 kb on - strand, within EX28DRAFT_1066 at 1077.293 kb on - strand, within EX28DRAFT_1066 at 1077.293 kb on - strand, within EX28DRAFT_1066 at 1077.348 kb on + strand, within EX28DRAFT_1066 at 1077.349 kb on - strand, within EX28DRAFT_1066 at 1077.664 kb on + strand, within EX28DRAFT_1066 at 1077.665 kb on - strand, within EX28DRAFT_1066 at 1077.771 kb on + strand, within EX28DRAFT_1066 at 1077.998 kb on - strand, within EX28DRAFT_1066 at 1078.293 kb on + strand, within EX28DRAFT_1066 at 1078.320 kb on + strand, within EX28DRAFT_1066 at 1078.435 kb on - strand, within EX28DRAFT_1066 at 1078.586 kb on - strand, within EX28DRAFT_1066 at 1078.797 kb on - strand, within EX28DRAFT_1066 at 1078.833 kb on + strand, within EX28DRAFT_1066 at 1078.937 kb on - strand at 1078.978 kb on + strand at 1078.979 kb on - strand at 1078.979 kb on - strand at 1078.981 kb on - strand at 1078.981 kb on - strand at 1079.034 kb on + strand at 1079.053 kb on + strand at 1079.056 kb on + strand at 1079.157 kb on - strand, within EX28DRAFT_1067 at 1079.407 kb on + strand, within EX28DRAFT_1067 at 1079.407 kb on + strand, within EX28DRAFT_1067 at 1079.637 kb on + strand at 1079.638 kb on - strand at 1079.638 kb on - strand at 1079.663 kb on + strand at 1079.678 kb on + strand at 1079.690 kb on + strand at 1079.707 kb on + strand at 1079.736 kb on + strand at 1079.739 kb on - strand at 1079.771 kb on + strand at 1079.771 kb on + strand at 1079.771 kb on + strand at 1079.771 kb on + strand at 1079.771 kb on + strand at 1079.771 kb on + strand at 1079.771 kb on + strand at 1079.771 kb on + strand at 1079.772 kb on - strand at 1079.772 kb on - strand at 1079.772 kb on - strand at 1079.772 kb on - strand at 1079.860 kb on + strand, within EX28DRAFT_1068 at 1079.860 kb on + strand, within EX28DRAFT_1068 at 1079.860 kb on + strand, within EX28DRAFT_1068 at 1079.861 kb on - strand, within EX28DRAFT_1068 at 1079.861 kb on - strand, within EX28DRAFT_1068 at 1079.891 kb on + strand, within EX28DRAFT_1068 at 1079.891 kb on + strand, within EX28DRAFT_1068 at 1079.892 kb on - strand, within EX28DRAFT_1068 at 1079.974 kb on - strand, within EX28DRAFT_1068
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,076,021 + EX28DRAFT_1064 0.25 -0.4 1,076,080 - EX28DRAFT_1064 0.34 -1.2 1,076,153 + EX28DRAFT_1064 0.45 -1.7 1,076,154 - EX28DRAFT_1064 0.45 -0.0 1,076,194 + EX28DRAFT_1064 0.52 +0.8 1,076,198 + EX28DRAFT_1064 0.52 +0.1 1,076,198 + EX28DRAFT_1064 0.52 +0.2 1,076,199 - EX28DRAFT_1064 0.52 -0.4 1,076,199 - EX28DRAFT_1064 0.52 +0.1 1,076,242 + EX28DRAFT_1064 0.59 +0.7 1,076,243 - EX28DRAFT_1064 0.59 +0.9 1,076,243 - EX28DRAFT_1064 0.59 +0.3 1,076,255 + EX28DRAFT_1064 0.61 +0.1 1,076,514 + -0.6 1,076,514 + +1.5 1,076,653 - EX28DRAFT_1065 0.52 +0.2 1,076,653 - EX28DRAFT_1065 0.52 +0.0 1,076,763 + EX28DRAFT_1065 0.89 +0.7 1,076,827 - -1.2 1,076,857 - -1.6 1,076,952 + +2.0 1,077,053 - +0.1 1,077,083 + +0.5 1,077,084 - +1.2 1,077,190 + EX28DRAFT_1066 0.11 +0.7 1,077,292 + EX28DRAFT_1066 0.16 -0.3 1,077,293 - EX28DRAFT_1066 0.16 -0.5 1,077,293 - EX28DRAFT_1066 0.16 +0.8 1,077,293 - EX28DRAFT_1066 0.16 +0.7 1,077,293 - EX28DRAFT_1066 0.16 -0.4 1,077,293 - EX28DRAFT_1066 0.16 -0.8 1,077,348 + EX28DRAFT_1066 0.19 +0.0 1,077,349 - EX28DRAFT_1066 0.19 +0.2 1,077,664 + EX28DRAFT_1066 0.34 -0.6 1,077,665 - EX28DRAFT_1066 0.34 +0.7 1,077,771 + EX28DRAFT_1066 0.39 +0.4 1,077,998 - EX28DRAFT_1066 0.50 +0.6 1,078,293 + EX28DRAFT_1066 0.64 -0.5 1,078,320 + EX28DRAFT_1066 0.65 +0.2 1,078,435 - EX28DRAFT_1066 0.70 +1.6 1,078,586 - EX28DRAFT_1066 0.78 -0.0 1,078,797 - EX28DRAFT_1066 0.88 +0.2 1,078,833 + EX28DRAFT_1066 0.89 +0.7 1,078,937 - +0.9 1,078,978 + +0.6 1,078,979 - +0.8 1,078,979 - +0.1 1,078,981 - -3.1 1,078,981 - +0.9 1,079,034 + -0.3 1,079,053 + -1.7 1,079,056 + +1.0 1,079,157 - EX28DRAFT_1067 0.12 +2.2 1,079,407 + EX28DRAFT_1067 0.55 -0.3 1,079,407 + EX28DRAFT_1067 0.55 +1.1 1,079,637 + +0.4 1,079,638 - +0.7 1,079,638 - +0.0 1,079,663 + -0.6 1,079,678 + -0.3 1,079,690 + -1.5 1,079,707 + -0.2 1,079,736 + -0.2 1,079,739 - -0.6 1,079,771 + -0.0 1,079,771 + +0.0 1,079,771 + +0.4 1,079,771 + -0.4 1,079,771 + -0.6 1,079,771 + +1.5 1,079,771 + +0.3 1,079,771 + +1.0 1,079,772 - +0.0 1,079,772 - -0.0 1,079,772 - +0.1 1,079,772 - +0.1 1,079,860 + EX28DRAFT_1068 0.10 +2.7 1,079,860 + EX28DRAFT_1068 0.10 -1.9 1,079,860 + EX28DRAFT_1068 0.10 -0.4 1,079,861 - EX28DRAFT_1068 0.11 -0.1 1,079,861 - EX28DRAFT_1068 0.11 -1.7 1,079,891 + EX28DRAFT_1068 0.14 -0.2 1,079,891 + EX28DRAFT_1068 0.14 +0.0 1,079,892 - EX28DRAFT_1068 0.14 +0.2 1,079,974 - EX28DRAFT_1068 0.24 +0.2
Or see this region's nucleotide sequence