Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0919 and EX28DRAFT_0920 are separated by 610 nucleotides EX28DRAFT_0920 and EX28DRAFT_0921 are separated by 41 nucleotides EX28DRAFT_0921 and EX28DRAFT_0922 are separated by 205 nucleotides
EX28DRAFT_0919: EX28DRAFT_0919 - hypothetical protein, at 920,646 to 921,272
_0919
EX28DRAFT_0920: EX28DRAFT_0920 - Transcriptional regulators, at 921,883 to 922,836
_0920
EX28DRAFT_0921: EX28DRAFT_0921 - Uncharacterized protein conserved in bacteria, at 922,878 to 923,174
_0921
EX28DRAFT_0922: EX28DRAFT_0922 - TonB family C-terminal domain, at 923,380 to 924,117
_0922
Position (kb) 921
922
923 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 920.945 kb on + strand, within EX28DRAFT_0919 at 920.964 kb on + strand, within EX28DRAFT_0919 at 920.965 kb on - strand, within EX28DRAFT_0919 at 920.965 kb on - strand, within EX28DRAFT_0919 at 920.997 kb on - strand, within EX28DRAFT_0919 at 921.010 kb on - strand, within EX28DRAFT_0919 at 921.064 kb on + strand, within EX28DRAFT_0919 at 921.086 kb on + strand, within EX28DRAFT_0919 at 921.104 kb on + strand, within EX28DRAFT_0919 at 921.129 kb on - strand, within EX28DRAFT_0919 at 921.129 kb on - strand, within EX28DRAFT_0919 at 921.151 kb on + strand, within EX28DRAFT_0919 at 921.151 kb on + strand, within EX28DRAFT_0919 at 921.152 kb on - strand, within EX28DRAFT_0919 at 921.152 kb on - strand, within EX28DRAFT_0919 at 921.152 kb on - strand, within EX28DRAFT_0919 at 921.161 kb on - strand, within EX28DRAFT_0919 at 921.161 kb on - strand, within EX28DRAFT_0919 at 921.161 kb on - strand, within EX28DRAFT_0919 at 921.166 kb on + strand, within EX28DRAFT_0919 at 921.196 kb on + strand, within EX28DRAFT_0919 at 921.234 kb on + strand at 921.235 kb on - strand at 921.304 kb on + strand at 921.305 kb on - strand at 921.305 kb on - strand at 921.437 kb on + strand at 921.437 kb on + strand at 921.452 kb on + strand at 921.452 kb on + strand at 921.499 kb on - strand at 921.499 kb on - strand at 921.501 kb on - strand at 921.534 kb on - strand at 921.573 kb on + strand at 921.594 kb on + strand at 921.595 kb on - strand at 921.595 kb on - strand at 921.642 kb on + strand at 921.661 kb on + strand at 921.661 kb on + strand at 921.681 kb on - strand at 921.681 kb on - strand at 921.821 kb on - strand at 921.823 kb on + strand at 921.823 kb on + strand at 921.882 kb on + strand at 921.882 kb on + strand at 921.883 kb on - strand at 921.983 kb on - strand, within EX28DRAFT_0920 at 922.016 kb on + strand, within EX28DRAFT_0920 at 922.022 kb on + strand, within EX28DRAFT_0920 at 922.131 kb on + strand, within EX28DRAFT_0920 at 922.355 kb on + strand, within EX28DRAFT_0920 at 922.356 kb on - strand, within EX28DRAFT_0920 at 922.356 kb on - strand, within EX28DRAFT_0920 at 922.359 kb on + strand, within EX28DRAFT_0920 at 922.360 kb on - strand, within EX28DRAFT_0920 at 922.366 kb on + strand, within EX28DRAFT_0920 at 922.366 kb on + strand, within EX28DRAFT_0920 at 922.367 kb on - strand, within EX28DRAFT_0920 at 922.369 kb on - strand, within EX28DRAFT_0920 at 922.369 kb on - strand, within EX28DRAFT_0920 at 922.369 kb on - strand, within EX28DRAFT_0920 at 922.412 kb on - strand, within EX28DRAFT_0920 at 922.479 kb on + strand, within EX28DRAFT_0920 at 922.602 kb on + strand, within EX28DRAFT_0920 at 922.714 kb on - strand, within EX28DRAFT_0920 at 922.834 kb on + strand at 922.841 kb on + strand at 922.893 kb on - strand at 922.894 kb on + strand at 923.065 kb on - strand, within EX28DRAFT_0921 at 923.207 kb on + strand at 923.207 kb on + strand at 923.208 kb on - strand at 923.213 kb on - strand at 923.240 kb on - strand at 923.260 kb on - strand at 923.260 kb on - strand at 923.386 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 920,945 + EX28DRAFT_0919 0.48 -0.8 920,964 + EX28DRAFT_0919 0.51 +0.1 920,965 - EX28DRAFT_0919 0.51 +0.6 920,965 - EX28DRAFT_0919 0.51 -0.8 920,997 - EX28DRAFT_0919 0.56 +0.5 921,010 - EX28DRAFT_0919 0.58 +0.1 921,064 + EX28DRAFT_0919 0.67 +0.6 921,086 + EX28DRAFT_0919 0.70 +1.2 921,104 + EX28DRAFT_0919 0.73 +0.1 921,129 - EX28DRAFT_0919 0.77 -1.5 921,129 - EX28DRAFT_0919 0.77 +1.6 921,151 + EX28DRAFT_0919 0.81 +0.4 921,151 + EX28DRAFT_0919 0.81 +0.6 921,152 - EX28DRAFT_0919 0.81 +2.8 921,152 - EX28DRAFT_0919 0.81 +1.8 921,152 - EX28DRAFT_0919 0.81 +0.4 921,161 - EX28DRAFT_0919 0.82 +0.0 921,161 - EX28DRAFT_0919 0.82 -1.2 921,161 - EX28DRAFT_0919 0.82 -2.7 921,166 + EX28DRAFT_0919 0.83 -0.7 921,196 + EX28DRAFT_0919 0.88 -1.0 921,234 + +0.3 921,235 - +1.6 921,304 + +0.5 921,305 - +0.5 921,305 - -0.8 921,437 + -0.8 921,437 + -0.1 921,452 + +0.6 921,452 + +0.9 921,499 - +0.6 921,499 - +1.0 921,501 - -0.0 921,534 - +0.7 921,573 + -2.3 921,594 + -0.3 921,595 - +0.5 921,595 - -0.8 921,642 + +2.2 921,661 + +0.2 921,661 + -0.5 921,681 - +0.1 921,681 - +0.7 921,821 - -0.1 921,823 + +0.2 921,823 + -3.8 921,882 + -0.2 921,882 + -0.3 921,883 - +0.5 921,983 - EX28DRAFT_0920 0.10 +2.0 922,016 + EX28DRAFT_0920 0.14 -0.2 922,022 + EX28DRAFT_0920 0.15 -3.3 922,131 + EX28DRAFT_0920 0.26 +0.0 922,355 + EX28DRAFT_0920 0.49 +0.5 922,356 - EX28DRAFT_0920 0.50 +1.9 922,356 - EX28DRAFT_0920 0.50 -0.8 922,359 + EX28DRAFT_0920 0.50 -0.8 922,360 - EX28DRAFT_0920 0.50 +0.7 922,366 + EX28DRAFT_0920 0.51 +0.2 922,366 + EX28DRAFT_0920 0.51 +0.5 922,367 - EX28DRAFT_0920 0.51 +0.2 922,369 - EX28DRAFT_0920 0.51 +1.1 922,369 - EX28DRAFT_0920 0.51 -0.9 922,369 - EX28DRAFT_0920 0.51 +0.1 922,412 - EX28DRAFT_0920 0.55 +1.8 922,479 + EX28DRAFT_0920 0.62 -0.4 922,602 + EX28DRAFT_0920 0.75 +0.3 922,714 - EX28DRAFT_0920 0.87 +1.4 922,834 + -1.2 922,841 + -1.8 922,893 - +0.2 922,894 + -0.3 923,065 - EX28DRAFT_0921 0.63 -1.2 923,207 + -0.1 923,207 + +0.9 923,208 - +1.0 923,213 - -0.5 923,240 - +0.6 923,260 - +0.3 923,260 - -0.9 923,386 - +1.2
Or see this region's nucleotide sequence