Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0856 and EX28DRAFT_0857 are separated by 652 nucleotides EX28DRAFT_0857 and EX28DRAFT_0858 are separated by 157 nucleotides EX28DRAFT_0858 and EX28DRAFT_0859 are separated by 46 nucleotides EX28DRAFT_0859 and EX28DRAFT_0860 are separated by 114 nucleotides
EX28DRAFT_0856: EX28DRAFT_0856 - Transcriptional regulators, at 868,464 to 868,907
_0856
EX28DRAFT_0857: EX28DRAFT_0857 - tRNA, at 869,560 to 869,641
_0857
EX28DRAFT_0858: EX28DRAFT_0858 - formyltetrahydrofolate deformylase, at 869,799 to 870,641
_0858
EX28DRAFT_0859: EX28DRAFT_0859 - Uncharacterized protein conserved in bacteria, at 870,688 to 871,146
_0859
EX28DRAFT_0860: EX28DRAFT_0860 - Predicted esterase of the alpha-beta hydrolase superfamily, at 871,261 to 872,163
_0860
Position (kb) 869
870
871 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 868.856 kb on + strand, within EX28DRAFT_0856 at 869.606 kb on - strand, within EX28DRAFT_0857 at 869.715 kb on - strand at 869.801 kb on - strand at 869.920 kb on + strand, within EX28DRAFT_0858 at 869.920 kb on + strand, within EX28DRAFT_0858 at 869.921 kb on - strand, within EX28DRAFT_0858 at 869.921 kb on - strand, within EX28DRAFT_0858 at 869.986 kb on + strand, within EX28DRAFT_0858 at 870.092 kb on + strand, within EX28DRAFT_0858 at 870.154 kb on + strand, within EX28DRAFT_0858 at 870.154 kb on + strand, within EX28DRAFT_0858 at 870.154 kb on + strand, within EX28DRAFT_0858 at 870.155 kb on - strand, within EX28DRAFT_0858 at 870.623 kb on + strand at 870.630 kb on + strand at 870.661 kb on + strand at 870.661 kb on + strand at 870.689 kb on + strand at 870.774 kb on + strand, within EX28DRAFT_0859 at 870.775 kb on - strand, within EX28DRAFT_0859 at 870.775 kb on - strand, within EX28DRAFT_0859 at 870.775 kb on - strand, within EX28DRAFT_0859 at 870.775 kb on - strand, within EX28DRAFT_0859 at 870.776 kb on + strand, within EX28DRAFT_0859 at 870.776 kb on + strand, within EX28DRAFT_0859 at 870.776 kb on + strand, within EX28DRAFT_0859 at 870.777 kb on - strand, within EX28DRAFT_0859 at 870.779 kb on + strand, within EX28DRAFT_0859 at 870.779 kb on + strand, within EX28DRAFT_0859 at 870.780 kb on - strand, within EX28DRAFT_0859 at 870.799 kb on + strand, within EX28DRAFT_0859 at 870.800 kb on - strand, within EX28DRAFT_0859 at 870.834 kb on + strand, within EX28DRAFT_0859 at 870.834 kb on + strand, within EX28DRAFT_0859 at 870.835 kb on - strand, within EX28DRAFT_0859 at 870.870 kb on - strand, within EX28DRAFT_0859 at 870.905 kb on + strand, within EX28DRAFT_0859 at 870.905 kb on + strand, within EX28DRAFT_0859 at 871.004 kb on + strand, within EX28DRAFT_0859 at 871.034 kb on + strand, within EX28DRAFT_0859 at 871.102 kb on + strand at 871.102 kb on + strand at 871.103 kb on - strand at 871.103 kb on - strand at 871.103 kb on - strand at 871.103 kb on - strand at 871.122 kb on + strand at 871.167 kb on + strand at 871.170 kb on + strand at 871.170 kb on + strand at 871.170 kb on + strand at 871.198 kb on + strand at 871.199 kb on - strand at 871.202 kb on + strand at 871.227 kb on - strand at 871.248 kb on + strand at 871.333 kb on - strand at 871.361 kb on + strand, within EX28DRAFT_0860 at 871.362 kb on - strand, within EX28DRAFT_0860 at 871.385 kb on + strand, within EX28DRAFT_0860 at 871.385 kb on + strand, within EX28DRAFT_0860 at 871.386 kb on - strand, within EX28DRAFT_0860
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 868,856 + EX28DRAFT_0856 0.88 +0.0 869,606 - EX28DRAFT_0857 0.56 +0.7 869,715 - -0.5 869,801 - +0.3 869,920 + EX28DRAFT_0858 0.14 -0.8 869,920 + EX28DRAFT_0858 0.14 -0.3 869,921 - EX28DRAFT_0858 0.14 -1.1 869,921 - EX28DRAFT_0858 0.14 +0.2 869,986 + EX28DRAFT_0858 0.22 -1.4 870,092 + EX28DRAFT_0858 0.35 -0.9 870,154 + EX28DRAFT_0858 0.42 +0.6 870,154 + EX28DRAFT_0858 0.42 -1.7 870,154 + EX28DRAFT_0858 0.42 -1.4 870,155 - EX28DRAFT_0858 0.42 -1.1 870,623 + -1.7 870,630 + -1.0 870,661 + -2.3 870,661 + -0.7 870,689 + +0.7 870,774 + EX28DRAFT_0859 0.19 -0.4 870,775 - EX28DRAFT_0859 0.19 -0.7 870,775 - EX28DRAFT_0859 0.19 +2.9 870,775 - EX28DRAFT_0859 0.19 +1.4 870,775 - EX28DRAFT_0859 0.19 -0.2 870,776 + EX28DRAFT_0859 0.19 -1.8 870,776 + EX28DRAFT_0859 0.19 -0.9 870,776 + EX28DRAFT_0859 0.19 -2.4 870,777 - EX28DRAFT_0859 0.19 -2.0 870,779 + EX28DRAFT_0859 0.20 -4.3 870,779 + EX28DRAFT_0859 0.20 -1.0 870,780 - EX28DRAFT_0859 0.20 +0.2 870,799 + EX28DRAFT_0859 0.24 +1.2 870,800 - EX28DRAFT_0859 0.24 +0.0 870,834 + EX28DRAFT_0859 0.32 -3.9 870,834 + EX28DRAFT_0859 0.32 -1.1 870,835 - EX28DRAFT_0859 0.32 -0.2 870,870 - EX28DRAFT_0859 0.40 -0.5 870,905 + EX28DRAFT_0859 0.47 +1.0 870,905 + EX28DRAFT_0859 0.47 -0.3 871,004 + EX28DRAFT_0859 0.69 -1.2 871,034 + EX28DRAFT_0859 0.75 -0.3 871,102 + +0.1 871,102 + +0.4 871,103 - -2.6 871,103 - -0.5 871,103 - -0.2 871,103 - +0.7 871,122 + -0.4 871,167 + -1.7 871,170 + +0.8 871,170 + +0.2 871,170 + -0.0 871,198 + +0.5 871,199 - +0.1 871,202 + +2.3 871,227 - -0.0 871,248 + -0.5 871,333 - -0.1 871,361 + EX28DRAFT_0860 0.11 -0.2 871,362 - EX28DRAFT_0860 0.11 -0.6 871,385 + EX28DRAFT_0860 0.14 -0.1 871,385 + EX28DRAFT_0860 0.14 -0.1 871,386 - EX28DRAFT_0860 0.14 -1.3
Or see this region's nucleotide sequence