Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0683 and EX28DRAFT_0684 are separated by 239 nucleotides EX28DRAFT_0684 and EX28DRAFT_0685 are separated by 78 nucleotides EX28DRAFT_0685 and EX28DRAFT_0686 are separated by 189 nucleotides
EX28DRAFT_0683: EX28DRAFT_0683 - arginyl-tRNA synthetase (EC 6.1.1.19), at 681,352 to 683,085
_0683
EX28DRAFT_0684: EX28DRAFT_0684 - Uncharacterized protein conserved in bacteria, at 683,325 to 683,885
_0684
EX28DRAFT_0685: EX28DRAFT_0685 - Uncharacterized protein involved in copper resistance, at 683,964 to 684,707
_0685
EX28DRAFT_0686: EX28DRAFT_0686 - tRNA (mo5U34)-methyltransferase, at 684,897 to 685,868
_0686
Position (kb)
683
684
685 Strain fitness (log2 ratio)
-1
0
1
2 at 683.224 kb on + strand at 683.224 kb on + strand at 683.225 kb on - strand at 683.225 kb on - strand at 683.230 kb on + strand at 683.245 kb on - strand at 683.287 kb on + strand at 683.360 kb on + strand at 683.737 kb on + strand, within EX28DRAFT_0684 at 683.883 kb on + strand at 683.962 kb on - strand at 683.989 kb on - strand at 684.082 kb on - strand, within EX28DRAFT_0685 at 684.162 kb on + strand, within EX28DRAFT_0685 at 684.338 kb on + strand, within EX28DRAFT_0685 at 684.339 kb on - strand, within EX28DRAFT_0685 at 684.479 kb on - strand, within EX28DRAFT_0685 at 684.572 kb on - strand, within EX28DRAFT_0685 at 684.713 kb on + strand at 684.746 kb on - strand at 684.809 kb on + strand at 685.271 kb on - strand, within EX28DRAFT_0686 at 685.493 kb on + strand, within EX28DRAFT_0686 at 685.494 kb on - strand, within EX28DRAFT_0686 at 685.494 kb on - strand, within EX28DRAFT_0686
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 683,224 + -0.3 683,224 + -0.4 683,225 - +1.9 683,225 - -0.4 683,230 + +0.4 683,245 - +0.3 683,287 + -0.4 683,360 + -0.0 683,737 + EX28DRAFT_0684 0.73 +0.3 683,883 + +0.1 683,962 - +0.1 683,989 - +0.6 684,082 - EX28DRAFT_0685 0.16 +0.3 684,162 + EX28DRAFT_0685 0.27 +0.2 684,338 + EX28DRAFT_0685 0.50 +0.4 684,339 - EX28DRAFT_0685 0.50 +1.0 684,479 - EX28DRAFT_0685 0.69 +1.3 684,572 - EX28DRAFT_0685 0.82 +0.1 684,713 + +0.4 684,746 - -0.5 684,809 + -0.3 685,271 - EX28DRAFT_0686 0.38 +1.0 685,493 + EX28DRAFT_0686 0.61 -0.1 685,494 - EX28DRAFT_0686 0.61 -0.6 685,494 - EX28DRAFT_0686 0.61 -0.8
Or see this region's nucleotide sequence