Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0624 and EX28DRAFT_0625 are separated by 129 nucleotides EX28DRAFT_0625 and EX28DRAFT_0626 are separated by 54 nucleotides EX28DRAFT_0626 and EX28DRAFT_0627 are separated by 86 nucleotides
EX28DRAFT_0624: EX28DRAFT_0624 - hypothetical protein, at 621,623 to 622,828
_0624
EX28DRAFT_0625: EX28DRAFT_0625 - RNA polymerase sigma factor, FliA/WhiG family, at 622,958 to 623,677
_0625
EX28DRAFT_0626: EX28DRAFT_0626 - flagellar regulatory protein FliZ, at 623,732 to 624,283
_0626
EX28DRAFT_0627: EX28DRAFT_0627 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 624,370 to 625,170
_0627
Position (kb)
622
623
624 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 622.039 kb on + strand, within EX28DRAFT_0624 at 622.039 kb on + strand, within EX28DRAFT_0624 at 622.039 kb on + strand, within EX28DRAFT_0624 at 622.039 kb on + strand, within EX28DRAFT_0624 at 622.040 kb on - strand, within EX28DRAFT_0624 at 622.175 kb on + strand, within EX28DRAFT_0624 at 622.253 kb on + strand, within EX28DRAFT_0624 at 622.322 kb on + strand, within EX28DRAFT_0624 at 622.515 kb on - strand, within EX28DRAFT_0624 at 622.556 kb on + strand, within EX28DRAFT_0624 at 622.557 kb on - strand, within EX28DRAFT_0624 at 622.609 kb on + strand, within EX28DRAFT_0624 at 622.620 kb on + strand, within EX28DRAFT_0624 at 622.638 kb on - strand, within EX28DRAFT_0624 at 622.638 kb on - strand, within EX28DRAFT_0624 at 622.645 kb on + strand, within EX28DRAFT_0624 at 622.696 kb on + strand, within EX28DRAFT_0624 at 622.697 kb on - strand, within EX28DRAFT_0624 at 622.699 kb on + strand, within EX28DRAFT_0624 at 622.741 kb on + strand at 622.774 kb on + strand at 622.775 kb on - strand at 622.776 kb on + strand at 622.776 kb on + strand at 622.776 kb on + strand at 622.776 kb on + strand at 622.777 kb on - strand at 622.777 kb on - strand at 622.777 kb on - strand at 622.939 kb on + strand at 622.939 kb on + strand at 622.940 kb on - strand at 622.954 kb on + strand at 622.970 kb on + strand at 622.971 kb on - strand at 622.986 kb on + strand at 622.986 kb on + strand at 622.987 kb on - strand at 623.015 kb on + strand at 623.015 kb on + strand at 623.016 kb on - strand at 623.115 kb on + strand, within EX28DRAFT_0625 at 623.115 kb on + strand, within EX28DRAFT_0625 at 623.116 kb on - strand, within EX28DRAFT_0625 at 623.159 kb on - strand, within EX28DRAFT_0625 at 623.166 kb on - strand, within EX28DRAFT_0625 at 623.348 kb on + strand, within EX28DRAFT_0625 at 623.374 kb on + strand, within EX28DRAFT_0625 at 623.375 kb on - strand, within EX28DRAFT_0625 at 623.375 kb on - strand, within EX28DRAFT_0625 at 623.377 kb on + strand, within EX28DRAFT_0625 at 623.393 kb on + strand, within EX28DRAFT_0625 at 623.475 kb on + strand, within EX28DRAFT_0625 at 623.475 kb on + strand, within EX28DRAFT_0625 at 623.475 kb on + strand, within EX28DRAFT_0625 at 623.512 kb on + strand, within EX28DRAFT_0625 at 623.544 kb on + strand, within EX28DRAFT_0625 at 623.544 kb on + strand, within EX28DRAFT_0625 at 623.545 kb on - strand, within EX28DRAFT_0625 at 623.555 kb on + strand, within EX28DRAFT_0625 at 623.556 kb on - strand, within EX28DRAFT_0625 at 623.556 kb on - strand, within EX28DRAFT_0625 at 623.676 kb on - strand at 623.729 kb on - strand at 623.750 kb on - strand at 623.785 kb on + strand at 623.816 kb on - strand, within EX28DRAFT_0626 at 624.059 kb on + strand, within EX28DRAFT_0626 at 624.060 kb on - strand, within EX28DRAFT_0626 at 624.107 kb on - strand, within EX28DRAFT_0626 at 624.206 kb on + strand, within EX28DRAFT_0626 at 624.207 kb on - strand, within EX28DRAFT_0626 at 624.216 kb on - strand, within EX28DRAFT_0626 at 624.216 kb on - strand, within EX28DRAFT_0626 at 624.240 kb on + strand at 624.275 kb on - strand at 624.276 kb on + strand at 624.276 kb on + strand at 624.277 kb on - strand at 624.277 kb on - strand at 624.278 kb on + strand at 624.278 kb on + strand at 624.279 kb on - strand at 624.340 kb on - strand at 624.355 kb on + strand at 624.356 kb on - strand at 624.356 kb on - strand at 624.356 kb on - strand at 624.369 kb on + strand at 624.480 kb on - strand, within EX28DRAFT_0627 at 624.643 kb on - strand, within EX28DRAFT_0627 at 624.670 kb on - strand, within EX28DRAFT_0627
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 622,039 + EX28DRAFT_0624 0.34 +0.6 622,039 + EX28DRAFT_0624 0.34 +0.7 622,039 + EX28DRAFT_0624 0.34 +1.7 622,039 + EX28DRAFT_0624 0.34 -0.6 622,040 - EX28DRAFT_0624 0.35 +0.2 622,175 + EX28DRAFT_0624 0.46 +1.1 622,253 + EX28DRAFT_0624 0.52 +0.1 622,322 + EX28DRAFT_0624 0.58 +1.2 622,515 - EX28DRAFT_0624 0.74 +2.1 622,556 + EX28DRAFT_0624 0.77 -0.4 622,557 - EX28DRAFT_0624 0.77 -1.1 622,609 + EX28DRAFT_0624 0.82 +1.3 622,620 + EX28DRAFT_0624 0.83 -0.7 622,638 - EX28DRAFT_0624 0.84 +3.1 622,638 - EX28DRAFT_0624 0.84 +0.5 622,645 + EX28DRAFT_0624 0.85 +0.2 622,696 + EX28DRAFT_0624 0.89 -0.4 622,697 - EX28DRAFT_0624 0.89 -0.9 622,699 + EX28DRAFT_0624 0.89 +0.7 622,741 + -0.6 622,774 + +0.3 622,775 - +0.2 622,776 + +0.1 622,776 + +0.3 622,776 + +0.1 622,776 + +1.0 622,777 - -0.6 622,777 - -0.4 622,777 - -0.1 622,939 + +0.7 622,939 + -0.8 622,940 - -0.8 622,954 + +0.8 622,970 + -0.3 622,971 - +0.2 622,986 + +0.3 622,986 + +0.9 622,987 - -1.3 623,015 + +0.4 623,015 + +0.1 623,016 - +0.2 623,115 + EX28DRAFT_0625 0.22 +1.1 623,115 + EX28DRAFT_0625 0.22 +0.3 623,116 - EX28DRAFT_0625 0.22 +0.4 623,159 - EX28DRAFT_0625 0.28 +0.5 623,166 - EX28DRAFT_0625 0.29 +1.2 623,348 + EX28DRAFT_0625 0.54 +0.2 623,374 + EX28DRAFT_0625 0.58 +0.8 623,375 - EX28DRAFT_0625 0.58 -0.3 623,375 - EX28DRAFT_0625 0.58 -0.3 623,377 + EX28DRAFT_0625 0.58 +0.8 623,393 + EX28DRAFT_0625 0.60 +0.8 623,475 + EX28DRAFT_0625 0.72 +1.4 623,475 + EX28DRAFT_0625 0.72 -1.1 623,475 + EX28DRAFT_0625 0.72 +0.8 623,512 + EX28DRAFT_0625 0.77 +0.6 623,544 + EX28DRAFT_0625 0.81 -0.2 623,544 + EX28DRAFT_0625 0.81 -0.3 623,545 - EX28DRAFT_0625 0.82 +0.3 623,555 + EX28DRAFT_0625 0.83 +0.8 623,556 - EX28DRAFT_0625 0.83 +0.4 623,556 - EX28DRAFT_0625 0.83 -0.2 623,676 - +1.8 623,729 - -0.2 623,750 - +0.5 623,785 + -0.1 623,816 - EX28DRAFT_0626 0.15 +0.2 624,059 + EX28DRAFT_0626 0.59 -0.3 624,060 - EX28DRAFT_0626 0.59 -0.0 624,107 - EX28DRAFT_0626 0.68 -0.0 624,206 + EX28DRAFT_0626 0.86 +1.7 624,207 - EX28DRAFT_0626 0.86 +0.7 624,216 - EX28DRAFT_0626 0.88 -0.9 624,216 - EX28DRAFT_0626 0.88 +0.9 624,240 + +0.6 624,275 - -1.6 624,276 + -0.2 624,276 + +0.3 624,277 - -0.1 624,277 - -1.6 624,278 + +0.8 624,278 + +0.6 624,279 - -0.2 624,340 - +0.2 624,355 + +0.3 624,356 - +0.6 624,356 - -1.6 624,356 - -0.0 624,369 + +0.1 624,480 - EX28DRAFT_0627 0.14 -3.4 624,643 - EX28DRAFT_0627 0.34 +0.1 624,670 - EX28DRAFT_0627 0.37 -1.5
Or see this region's nucleotide sequence