Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0529 and EX28DRAFT_0530 are separated by 226 nucleotides EX28DRAFT_0530 and EX28DRAFT_0531 are separated by 52 nucleotides
EX28DRAFT_0529: EX28DRAFT_0529 - 6-phosphogluconate dehydrogenase (decarboxylating), at 523,019 to 524,425
_0529
EX28DRAFT_0530: EX28DRAFT_0530 - nucleotide sugar dehydrogenase, at 524,652 to 525,818
_0530
EX28DRAFT_0531: EX28DRAFT_0531 - Nucleoside-diphosphate-sugar epimerases, at 525,871 to 526,875
_0531
Position (kb) 524
525
526 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 523.880 kb on + strand, within EX28DRAFT_0529 at 524.192 kb on + strand, within EX28DRAFT_0529 at 524.193 kb on - strand, within EX28DRAFT_0529 at 524.371 kb on + strand at 524.375 kb on + strand at 524.408 kb on - strand at 524.423 kb on + strand at 524.428 kb on + strand at 524.507 kb on - strand at 524.507 kb on - strand at 524.536 kb on + strand at 524.591 kb on + strand at 524.591 kb on + strand at 524.591 kb on + strand at 524.591 kb on + strand at 524.591 kb on + strand at 524.592 kb on - strand at 525.960 kb on - strand at 526.103 kb on + strand, within EX28DRAFT_0531 at 526.176 kb on + strand, within EX28DRAFT_0531 at 526.177 kb on - strand, within EX28DRAFT_0531 at 526.195 kb on - strand, within EX28DRAFT_0531 at 526.220 kb on + strand, within EX28DRAFT_0531 at 526.221 kb on - strand, within EX28DRAFT_0531 at 526.248 kb on - strand, within EX28DRAFT_0531 at 526.251 kb on + strand, within EX28DRAFT_0531 at 526.253 kb on + strand, within EX28DRAFT_0531 at 526.290 kb on + strand, within EX28DRAFT_0531 at 526.291 kb on - strand, within EX28DRAFT_0531 at 526.296 kb on + strand, within EX28DRAFT_0531 at 526.331 kb on + strand, within EX28DRAFT_0531 at 526.371 kb on - strand, within EX28DRAFT_0531 at 526.371 kb on - strand, within EX28DRAFT_0531 at 526.383 kb on - strand, within EX28DRAFT_0531 at 526.489 kb on + strand, within EX28DRAFT_0531 at 526.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 523,880 + EX28DRAFT_0529 0.61 +0.5 524,192 + EX28DRAFT_0529 0.83 -1.4 524,193 - EX28DRAFT_0529 0.83 +0.2 524,371 + +1.1 524,375 + -4.1 524,408 - -0.1 524,423 + +2.2 524,428 + -0.3 524,507 - -2.5 524,507 - +1.0 524,536 + +0.1 524,591 + -3.5 524,591 + +0.5 524,591 + -0.1 524,591 + -1.5 524,591 + -0.2 524,592 - -1.2 525,960 - -1.4 526,103 + EX28DRAFT_0531 0.23 -0.1 526,176 + EX28DRAFT_0531 0.30 -1.9 526,177 - EX28DRAFT_0531 0.30 -1.2 526,195 - EX28DRAFT_0531 0.32 -0.2 526,220 + EX28DRAFT_0531 0.35 -0.0 526,221 - EX28DRAFT_0531 0.35 -0.6 526,248 - EX28DRAFT_0531 0.38 -2.1 526,251 + EX28DRAFT_0531 0.38 -2.2 526,253 + EX28DRAFT_0531 0.38 -0.5 526,290 + EX28DRAFT_0531 0.42 -2.0 526,291 - EX28DRAFT_0531 0.42 -0.5 526,296 + EX28DRAFT_0531 0.42 -1.4 526,331 + EX28DRAFT_0531 0.46 -0.9 526,371 - EX28DRAFT_0531 0.50 +0.0 526,371 - EX28DRAFT_0531 0.50 -2.1 526,383 - EX28DRAFT_0531 0.51 +1.0 526,489 + EX28DRAFT_0531 0.61 -2.1 526,793 - -1.0
Or see this region's nucleotide sequence