Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0515 and EX28DRAFT_0516 are separated by 53 nucleotides EX28DRAFT_0516 and EX28DRAFT_0517 overlap by 1 nucleotides
EX28DRAFT_0515: EX28DRAFT_0515 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 508,489 to 509,571
_0515
EX28DRAFT_0516: EX28DRAFT_0516 - Nucleoside-diphosphate-sugar epimerases, at 509,625 to 510,569
_0516
EX28DRAFT_0517: EX28DRAFT_0517 - UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase, at 510,569 to 511,603
_0517
Position (kb) 509
510
511 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 509.192 kb on - strand, within EX28DRAFT_0515 at 509.533 kb on + strand at 509.586 kb on + strand at 509.599 kb on - strand at 509.704 kb on + strand at 509.762 kb on + strand, within EX28DRAFT_0516 at 509.807 kb on + strand, within EX28DRAFT_0516 at 509.838 kb on - strand, within EX28DRAFT_0516 at 509.962 kb on + strand, within EX28DRAFT_0516 at 509.963 kb on - strand, within EX28DRAFT_0516 at 510.033 kb on + strand, within EX28DRAFT_0516 at 510.038 kb on + strand, within EX28DRAFT_0516 at 510.039 kb on - strand, within EX28DRAFT_0516 at 510.039 kb on - strand, within EX28DRAFT_0516 at 510.106 kb on + strand, within EX28DRAFT_0516 at 510.381 kb on + strand, within EX28DRAFT_0516 at 510.993 kb on - strand, within EX28DRAFT_0517 at 511.034 kb on - strand, within EX28DRAFT_0517 at 511.093 kb on - strand, within EX28DRAFT_0517 at 511.093 kb on - strand, within EX28DRAFT_0517 at 511.334 kb on - strand, within EX28DRAFT_0517 at 511.337 kb on - strand, within EX28DRAFT_0517
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 509,192 - EX28DRAFT_0515 0.65 -0.5 509,533 + +0.2 509,586 + -0.5 509,599 - +0.9 509,704 + -1.9 509,762 + EX28DRAFT_0516 0.14 +1.1 509,807 + EX28DRAFT_0516 0.19 +0.9 509,838 - EX28DRAFT_0516 0.23 +0.8 509,962 + EX28DRAFT_0516 0.36 +1.0 509,963 - EX28DRAFT_0516 0.36 -0.3 510,033 + EX28DRAFT_0516 0.43 +1.2 510,038 + EX28DRAFT_0516 0.44 +0.8 510,039 - EX28DRAFT_0516 0.44 -0.0 510,039 - EX28DRAFT_0516 0.44 -4.4 510,106 + EX28DRAFT_0516 0.51 +0.1 510,381 + EX28DRAFT_0516 0.80 +0.5 510,993 - EX28DRAFT_0517 0.41 +0.5 511,034 - EX28DRAFT_0517 0.45 -0.3 511,093 - EX28DRAFT_0517 0.51 +0.5 511,093 - EX28DRAFT_0517 0.51 -0.5 511,334 - EX28DRAFT_0517 0.74 +2.7 511,337 - EX28DRAFT_0517 0.74 +0.4
Or see this region's nucleotide sequence