Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0440 and EX28DRAFT_0441 are separated by 94 nucleotides EX28DRAFT_0441 and EX28DRAFT_0442 overlap by 26 nucleotides EX28DRAFT_0442 and EX28DRAFT_0443 are separated by 487 nucleotides
EX28DRAFT_0440: EX28DRAFT_0440 - ATPases involved in chromosome partitioning, at 421,549 to 422,658
_0440
EX28DRAFT_0441: EX28DRAFT_0441 - Acetyltransferases, at 422,753 to 423,208
_0441
EX28DRAFT_0442: EX28DRAFT_0442 - Predicted integral membrane protein, at 423,183 to 423,515
_0442
EX28DRAFT_0443: EX28DRAFT_0443 - Periplasmic protein involved in polysaccharide export, at 424,003 to 425,143
_0443
Position (kb)
422
423
424 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 421.918 kb on - strand, within EX28DRAFT_0440 at 421.987 kb on + strand, within EX28DRAFT_0440 at 421.987 kb on + strand, within EX28DRAFT_0440 at 421.987 kb on + strand, within EX28DRAFT_0440 at 421.988 kb on - strand, within EX28DRAFT_0440 at 421.988 kb on - strand, within EX28DRAFT_0440 at 422.201 kb on - strand, within EX28DRAFT_0440 at 422.201 kb on - strand, within EX28DRAFT_0440 at 422.290 kb on - strand, within EX28DRAFT_0440 at 422.421 kb on + strand, within EX28DRAFT_0440 at 422.422 kb on - strand, within EX28DRAFT_0440 at 422.461 kb on + strand, within EX28DRAFT_0440 at 422.462 kb on - strand, within EX28DRAFT_0440 at 422.462 kb on - strand, within EX28DRAFT_0440 at 422.462 kb on - strand, within EX28DRAFT_0440 at 422.462 kb on - strand, within EX28DRAFT_0440 at 422.778 kb on + strand at 422.778 kb on + strand at 422.779 kb on - strand at 422.779 kb on - strand at 422.779 kb on - strand at 422.779 kb on - strand at 422.782 kb on - strand at 422.782 kb on - strand at 422.872 kb on + strand, within EX28DRAFT_0441 at 423.200 kb on + strand at 423.200 kb on + strand at 423.200 kb on + strand at 423.212 kb on - strand at 423.253 kb on + strand, within EX28DRAFT_0442 at 423.253 kb on + strand, within EX28DRAFT_0442 at 423.253 kb on + strand, within EX28DRAFT_0442 at 423.253 kb on + strand, within EX28DRAFT_0442 at 423.254 kb on - strand, within EX28DRAFT_0442 at 423.254 kb on - strand, within EX28DRAFT_0442 at 423.268 kb on + strand, within EX28DRAFT_0442 at 423.268 kb on + strand, within EX28DRAFT_0442 at 423.269 kb on - strand, within EX28DRAFT_0442 at 423.269 kb on - strand, within EX28DRAFT_0442 at 423.269 kb on - strand, within EX28DRAFT_0442 at 423.269 kb on - strand, within EX28DRAFT_0442 at 423.309 kb on + strand, within EX28DRAFT_0442 at 423.310 kb on - strand, within EX28DRAFT_0442 at 423.310 kb on - strand, within EX28DRAFT_0442 at 423.316 kb on + strand, within EX28DRAFT_0442 at 423.331 kb on + strand, within EX28DRAFT_0442 at 423.412 kb on + strand, within EX28DRAFT_0442 at 423.413 kb on - strand, within EX28DRAFT_0442 at 423.480 kb on + strand, within EX28DRAFT_0442 at 423.481 kb on - strand, within EX28DRAFT_0442 at 423.483 kb on + strand at 423.484 kb on - strand at 423.484 kb on - strand at 423.491 kb on + strand at 423.593 kb on - strand at 423.606 kb on - strand at 423.800 kb on + strand at 423.821 kb on + strand at 423.822 kb on - strand at 423.887 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 421,918 - EX28DRAFT_0440 0.33 -0.2 421,987 + EX28DRAFT_0440 0.39 +0.6 421,987 + EX28DRAFT_0440 0.39 -1.0 421,987 + EX28DRAFT_0440 0.39 +0.7 421,988 - EX28DRAFT_0440 0.40 +0.3 421,988 - EX28DRAFT_0440 0.40 +0.3 422,201 - EX28DRAFT_0440 0.59 +0.1 422,201 - EX28DRAFT_0440 0.59 -1.4 422,290 - EX28DRAFT_0440 0.67 +0.0 422,421 + EX28DRAFT_0440 0.79 -0.2 422,422 - EX28DRAFT_0440 0.79 +0.9 422,461 + EX28DRAFT_0440 0.82 +0.1 422,462 - EX28DRAFT_0440 0.82 -3.4 422,462 - EX28DRAFT_0440 0.82 -0.1 422,462 - EX28DRAFT_0440 0.82 -0.2 422,462 - EX28DRAFT_0440 0.82 -1.0 422,778 + +1.1 422,778 + -1.1 422,779 - +0.8 422,779 - -0.5 422,779 - +1.2 422,779 - -0.3 422,782 - +0.8 422,782 - -1.0 422,872 + EX28DRAFT_0441 0.26 +0.7 423,200 + -0.1 423,200 + +0.0 423,200 + -0.6 423,212 - -3.3 423,253 + EX28DRAFT_0442 0.21 -1.5 423,253 + EX28DRAFT_0442 0.21 -0.3 423,253 + EX28DRAFT_0442 0.21 +1.1 423,253 + EX28DRAFT_0442 0.21 -0.6 423,254 - EX28DRAFT_0442 0.21 +0.2 423,254 - EX28DRAFT_0442 0.21 -0.1 423,268 + EX28DRAFT_0442 0.26 -0.1 423,268 + EX28DRAFT_0442 0.26 +1.0 423,269 - EX28DRAFT_0442 0.26 +0.0 423,269 - EX28DRAFT_0442 0.26 -0.1 423,269 - EX28DRAFT_0442 0.26 -0.2 423,269 - EX28DRAFT_0442 0.26 +0.0 423,309 + EX28DRAFT_0442 0.38 +0.3 423,310 - EX28DRAFT_0442 0.38 -1.3 423,310 - EX28DRAFT_0442 0.38 +0.5 423,316 + EX28DRAFT_0442 0.40 -0.8 423,331 + EX28DRAFT_0442 0.44 -0.3 423,412 + EX28DRAFT_0442 0.69 +0.3 423,413 - EX28DRAFT_0442 0.69 -0.5 423,480 + EX28DRAFT_0442 0.89 -2.6 423,481 - EX28DRAFT_0442 0.89 +0.2 423,483 + -0.6 423,484 - +0.7 423,484 - +0.3 423,491 + +1.3 423,593 - +0.4 423,606 - -0.2 423,800 + -0.2 423,821 + +0.0 423,822 - +0.0 423,887 + -0.1
Or see this region's nucleotide sequence